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Plant cell differentiation promoter

A differentiation accelerator and plant cell technology, applied in plant cells, plant growth regulators, plant growth regulators, etc., can solve the problem that the effect is difficult to say is sufficient, and achieve the effect of stable efficiency

Inactive Publication Date: 2014-03-12
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these methods are limited to species and / or physiological factors, and their effects are hardly sufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Induction of callus tissue from cultured seedlings of the genus Bractia

[0063] As cultured seedlings of the genus Brachyphyllum (species Double Take), MS medium (medium of Murashige and Skoog) added to a planting box (inner volume 300 ml) manufactured by Asahi Technoglass Co., Ltd. with 3% sucrose, Agar (Wako Pure Chemical Industries, Tokyo, Japan) 0.8% and the solid medium adjusted to pH 5.8, in the bright place (photosynthetic photon beam density 5.7μmole / m 2 / sec, 16 hours of day length), the cultured seedlings were subcultured and maintained under the conditions of 25°C.

[0064] Sucrose (3%), 2,4-dichlorophenoxyacetic acid (4ppm), benzyl adenine (BA) (0.2ppm), acid hydrolyzate of casein were added to MS medium solidified with 0.8% agar (100ppm), 5mM 2-morpholineethanesulfonic acid monohydrate (MES) to prepare a medium for callus induction and proliferation (hereinafter referred to as "subculture medium") (pH 5.8). From the cultured seedlings cultur...

Embodiment 2

[0066] Example 2: Effect of KODA on Adventitious Embryo Differentiation Induction from Callus of the genus Brachyphyllum ring

[0067] The embryogenic callus maintained in the subculture medium was subcultured in a new subculture medium, and after 1 week (7 days), 2 weeks (14 days), and 3 weeks (20 days) after the callus 3ppm aqueous solution of KODA was added dropwise to the wounded tissue. After 3 weeks from the start of subculture, the cultured callus was placed in a liquid redifferentiation medium, and differentiated adventitious embryos were recovered after 6 weeks ( figure 1 ), carry out dehydration, measure the adventitious embryo weight after dehydration. The adventitious embryos were placed on the germination medium, and the number of germination bodies obtained was counted. The results are shown in Table 1. Calculate the germination body number corresponding to the adventitious embryo weight after every 1g dehydration ( figure 2 ). Compared with the control...

Embodiment 3

[0070] Example 3: KODA Concentration in Induction of Adventitious Embryo Differentiation from Callus of Colocasia genus Impact

[0071] The embryogenic callus maintained in the subculture medium was placed in a new redifferentiation medium, and after 2 weeks, 0.03ppm, 0.3ppm, and 3ppm of KODA aqueous solution were added dropwise, and the number of normally regenerated plants was recorded after 8 weeks. . Although plant bodies were regenerated in all test plots, a large number of plant bodies with severe stress symptoms (thin leaves) were found ( image 3). Those plants showing severe stress symptoms were not counted in the number of normally regenerated plants. In the control area, the number of plants showing stress symptoms was large, and as a result, the number of normally regenerated plants was small. The results are shown in Figure 4 .

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Abstract

The present invention addresses the problem of providing a plant cell differentiation promoter with which it is possible to promote differentiation from a callus to a normal adventitious embryo, or promote differentiation of an adventitious root or adventitious bud from a plant cutting, and as a result, obtain a regenerated plant with stability. The present invention provides a plant differentiation promoter comprising as the active ingredient a specific ketole fatty acid or derivative thereof.

Description

technical field [0001] The present invention relates to a plant cell differentiation promoter, which comprises a ketol fatty acid with 4 to 24 carbon atoms, in which the carbonyl group is The carbon atom and the carbon atom bound to the hydroxyl group are in the alpha position. The present invention further relates to a method for preparing regenerated plant bodies from callus tissue or plant slices, which includes the step of adding specific ketol fatty acids. Background technique [0002] Tissue culture techniques utilizing the totipotency possessed by plants have become indispensable in breeding for the purpose of increasing the yield of homogeneous superior clones, reproducing virus-free plants, and producing new varieties. By using plant tissue culture techniques, the callus is proliferated, and then the concentration ratio of auxin (auxin) and cytokines in the medium is changed for the proliferated tissue, so that it can undergo organ differentiation into adventitious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01H4/00A01N37/42A01P21/00
CPCC12N5/04A01H4/00A01N37/42
Inventor 横山峰幸高木一辉大西升绳田由纪子
Owner SHISEIDO CO LTD
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