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Method for extraction of Auricularia auricula polysaccharide from Auricularia auricula

A fungus polysaccharide and fungus technology, applied in the field of extracting fungus polysaccharide, can solve the problems of reduced bioavailability, coarseness, easy to destroy polysaccharide structure and the like

Active Publication Date: 2014-03-19
威海市桢昊生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fungus cells are thick and the wall is thick, so it is difficult to dissolve the polysaccharides from the water extraction; and the acid-base extraction method is easy to destroy the polysaccharide structure and reduce its bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting fungus polysaccharides from fungus, through the following process steps:

[0019] a. Selection of raw materials: 100kg of mature, disease-free dry fungus is selected as raw materials for use;

[0020] b. Raw material processing: Add the fungus raw materials selected in step (a) to 8 times the quality of the fungus, soak for 3.5 hours, then rinse with clean water, and then send it to the tissue masher and add water 3 times the quality of the fungus to mash into a slurry Shape, get fungus slurry;

[0021] c. Wall breakage by enzymatic hydrolysis. Add the fungus slurry obtained in step (b) to 4.5 times the quality of the fungus, stir evenly, control the temperature to 48°C, adjust the pH to 6.8, and add cellulase with a liquid solid content of 0.3%. Enzymatic hydrolysis, enzymatic hydrolysis time is 2.5h; after enzymatic hydrolysis is completed, the temperature is raised to 95℃, kept for 25min, and the enzyme is inactivated; the fungus solid-liquid mixtur...

Embodiment 2

[0028] A method for extracting fungus polysaccharides from fungus, through the following process steps:

[0029] a. Selection of raw materials: 200kg of mature, disease-free, dry fungus is selected as raw materials for use;

[0030] b. Raw material processing: Add the fungus material selected in step (a) to water 10 times the mass of the fungus, soak for 3 hours, then rinse with clean water, and then send it to the tissue masher and add water twice the mass of the fungus to mash into a slurry , Get fungus slurry;

[0031] c. Enzymatic hydrolysis to break the wall. Add the fungus slurry obtained in step (b) to 5 times the quality of the fungus in water, stir evenly, control the temperature at 50°C, adjust the pH to 6.5, and add cellulase with 0.5% liquid solid content to proceed. Enzymatic hydrolysis, enzymatic hydrolysis time is 2h; after enzymatic hydrolysis is completed, the temperature is raised to 100℃, kept for 20min, and the enzyme is inactivated; the fungus solid-liquid mixtu...

Embodiment 3

[0038] A method for extracting fungus polysaccharides from fungus, through the following process steps:

[0039] a. Selection of raw materials: 100kg of mature, disease-free dry fungus is selected as raw materials for use;

[0040] b. Raw material processing: Add the fungus raw material selected in step (a) to 5 times the quality of the fungus, soak it for 4 hours, then rinse it with clean water, and then send it to the tissue masher and add water 4 times the quality of the fungus to mash it into a slurry , Get fungus slurry;

[0041] c. Wall breakage by enzymatic hydrolysis. Add the fungus slurry obtained in step (b) to 4 times the quality of the fungus in water, stir evenly, control the temperature at 45°C, adjust the pH to 7.0, and add cellulase with a liquid solid content of 0.2%. Enzymolysis, the enzymolysis time is 3h; after enzymolysis is completed, the temperature is raised to 90℃, kept for 30min, and the enzyme is inactivated; the agaric solid-liquid mixture after enzymolys...

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PUM

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Abstract

The invention relates to a method for extraction of Auricularia auricula polysaccharide from Auricularia auricula. The method includes: selecting dry Auricularia auricula as raw materials, conducting soaking, washing and mashing, then adding cellulase, controlling enzymolysis conditions and performing enzymolysis to break cell walls; subjecting the enzymolysis generated Auricularia auricula residue to hot water extraction; combining an Auricularia auricula enzymolysis liquid and an Auricularia auricula extract liquid, conducting filtering, then adding a tannic acid solution to remove protein from the filtered liquid, subjecting a polysaccharide solution collected by centrifugation to alcohol precipitation by an ethanol solution; and finally, carrying out vacuum drying to obtain a polysaccharide product. The extraction method provided by the invention has the advantages of reasonable process, simple operation and comprehensive component retaining, the polysaccharide yield and purity are high, and the product safety is good.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a method for extracting polysaccharides from fungus. Background technique [0002] We know that fungus belongs to Basidiomycetes, Auricularia and Agaricaceae in mycological classification. The color is dark brown, the texture is soft, and it can prevent iron deficiency anemia and other medicinal effects. Mainly distributed in Heilongjiang, Jilin, Fujian, Taiwan, Hubei, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan and other places. The fruiting body of fungus contains acidic mucopolysaccharides, which are composed of monosaccharides such as L-fucose, L-arabinose, D-xylose, D-mannose, D-glucose and glucuronic acid. The fungus polysaccharide extracted from the fungus has the effects of lowering blood sugar, blood fat, lowering cholesterol, inhibiting platelet aggregation, improving the immune function of the body, anti-thrombosis, anti-aging, anti-ulcer, and anti-radiation. ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 倪海平唐茂林杨众唐严芳
Owner 威海市桢昊生物技术有限公司
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