Method for preparing water-soluble chitosan oligosaccharide by adopting ultrafiltration and nanofiltration

A technology of water-soluble chitosan oligosaccharides and chitosan oligosaccharides, applied in chemical instruments and methods, preparation of sugar derivatives, amino sugars, etc., can solve the problems of complex hydration process and high cost, and achieve simple degradation method, low cost, and improved The effect of degradation efficiency

Active Publication Date: 2014-03-19
上海长欧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its hydration process is complicated, requiring additional use of acid and alkali, resulting in high cost

Method used

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  • Method for preparing water-soluble chitosan oligosaccharide by adopting ultrafiltration and nanofiltration
  • Method for preparing water-soluble chitosan oligosaccharide by adopting ultrafiltration and nanofiltration
  • Method for preparing water-soluble chitosan oligosaccharide by adopting ultrafiltration and nanofiltration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. After rinsing and drying, 10 kg of chitosan raw materials are put into a special microwave reactor for microwave radiation treatment, the microwave power is 1000W, the temperature is controlled at 60°C, and the reaction time is 1 hour.

[0056] 2. Add 35 liters of water to the reaction vessel and heat the water to 40°C; then add 10 kg of chitosan treated with microwave radiation into the vessel, stir evenly, and make the raw materials evenly infiltrated.

[0057] 3. Add 15,000 ml of hydrogen peroxide (35% by mass) solution to the chitosan infiltration solution in 4-5 times, stir while adding, and mix well. The degradation reaction of chitosan begins, and the material slowly rises accordingly. During the degradation process, the temperature is controlled within 80°C. After the reaction has proceeded for 4 hours, the degradation is complete, and it is cooled to normal temperature. Use qualitative filter paper to filter under negative pressure to separate the undegraded chi...

Embodiment 2

[0067] 1. After rinsing and drying, 10 kg of chitosan raw materials are put into a special microwave reactor for microwave radiation treatment, the microwave power is 1000W, the temperature is controlled at 60°C, and the reaction time is 1 hour.

[0068] 2. Add 35 liters of water to the reaction vessel and heat the water to 40°C; then add 10 kg of chitosan treated with microwave radiation into the vessel, stir evenly, and make the raw materials evenly infiltrated.

[0069] 3. Add 15,000 ml of hydrogen peroxide (50% by mass) solution to the chitosan infiltration solution in 4-5 times, stir while adding, and mix well. The degradation reaction of chitosan begins, and the material slowly rises accordingly. During the degradation process, the temperature is controlled within 80°C. After the reaction was carried out for 3 hours, the degradation was over, and it was cooled to normal temperature. Use qualitative filter paper to filter under negative pressure to separate the undegraded chi...

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Abstract

The invention relates to a method for preparing water-soluble chitosan oligosaccharide by adopting ultrafiltration and nanofiltration. The method comprises the following steps: (1) preparing a chitosan oligosaccharide solution by using microwave hydrogen peroxide to perform compound degradation on chitosan; (2) separating out an active oligosaccharide concentrated solution by adopting an ultrafiltration and nanofiltration separation technology; (3) performing spray drying to obtain a chitosan oligosaccharide powder finished product. According to the method provided by the invention, the integrated production of reaction and separation of the finished product is realized, and the secondary degradation of active oligosaccharide is prevented. As the process realizes continuous operation, no other reaction byproducts are generated in the production process, and no pollutants are produced; compared with the preparation method in the prior art, the method has the advantages of advanced production process, simple generation process steps, low investment in production facilities, low cost, environmental friendliness, energy saving, and capability of rapidly realizing industrial production.

Description

Technical field [0001] The invention belongs to the field of water-soluble chito-oligosaccharides, and particularly relates to a method for preparing water-soluble chito-oligosaccharides by ultrafiltration and nanofiltration. Background technique [0002] Chitosan oligosaccharide, also known as oligochitosan, is a natural and highly active animal cellulose product made from chitosan obtained by deacetylation of chitin as a raw material through high-tech degradation and separation methods. Because of its unique pharmacological effects and the resulting broad market application prospects, it has attracted increasing attention. [0003] Chitin is a pure natural polymer compound, which is widely present in crustaceans such as shrimps and crabs, insects, and algae. Its annual biosynthetic resources are as high as 10 billion tons, and the amount of marine organisms is about 10 Over 100 million tons, it is the second inexhaustible natural biological resource on the earth after plant fibe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C07H5/06C07H1/00
Inventor 吴彪
Owner 上海长欧生物科技有限公司
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