A kind of composite bacterial flora for degrading papermaking wastewater and its preparation method

A technology for compound flora and papermaking wastewater, applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2016-06-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, and it At a disadvantage in interspecies competition, traditional biological treatment technologies face great challenges

Method used

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  • A kind of composite bacterial flora for degrading papermaking wastewater and its preparation method
  • A kind of composite bacterial flora for degrading papermaking wastewater and its preparation method
  • A kind of composite bacterial flora for degrading papermaking wastewater and its preparation method

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Effect test

Embodiment 1

[0023]Pick Agrobacterium sp., Bacillus sp., Enterobacter.cloacae., Pseudomonasputida., Pseudomonasstutzeri. Separated from the sludge of the drainage outlet of the factory) five kinds of bacteria 1 ring: transfer them to 20mL nutrient solution (the composition is 1.5g / L beef extract, 1.0g / L glucose, 5.5g / L tryptone , 3.0g / L yeast powder, pH 6.5, and the rest is water), each kind of bacteria was cultured at 27°C for 2 days, and then the five cultured bacteria were cultured at a volume ratio of 1:9 (bacteria Cells and proliferation medium) were inoculated into a container containing 300mL of proliferation medium, cultured at 27°C for 2 days, and finally centrifuged at 6000rpm for 15min to obtain the logarithmic growth phase cells of the above five kinds of bacteria respectively. After washing with normal saline, dry and refrigerate for later use. Among them, five different bacteria use different proliferation media. The nutrient media of Agrobacterium, rod-shaped bacteria and E...

Embodiment 2

[0032] Pick two rings of five kinds of bacteria respectively: Agrobacteriumsp., Bacillussp., Enterobacter.Cloacae., Pseudomonasputida., Pseudomonas stutzeri ( Pseudomonasstutzeri.) (separated from the sludge at the outlet of the paper mill) were transferred to 30 mL of nutrient solution (the composition of which was 2.0 g / L beef extract, 1.5 g / L glucose, 6.5 g / L tryptone , 3.5g / L yeast powder, pH7.5, and the rest is water), each kind of bacteria was cultured at 30°C for 2 days, and then the cultured five kinds of bacteria were respectively mixed in a volume ratio of 1:10 ( Bacteria and proliferation medium) were inoculated into a container containing 500mL of proliferation medium, cultured at 35°C for 1 day, and finally centrifuged at 7000rpm for 10min to obtain the logarithmic growth phase cells of the above five kinds of bacteria , rinsed with saline, dried and refrigerated for later use. Among them, five different bacteria use different proliferation media. The nutrient me...

Embodiment 3

[0041] Pick two rings of five kinds of bacteria respectively: Agrobacteriumsp., Bacillussp., Enterobacter.Cloacae., Pseudomonasputida., Pseudomonas stutzeri ( Pseudomonasstutzeri.) (separated from the sludge at the outlet of the paper mill) were transferred to 25 mL of nutrient solution (the composition of which was 1.7 g / L beef extract, 2.0 g / L glucose, 6.0 g / L tryptone , 4.0g / L yeast powder, pH 7.0, and the rest is water), each kind of bacteria was cultured at 30°C for 2 days, and then the cultured five kinds of bacteria were respectively mixed in a volume ratio of 1:12 ( Bacteria and proliferation medium) were inoculated into a container containing 400mL of proliferation medium, cultured at 35°C for 2 days, and finally centrifuged at 7000rpm for 10min to obtain the logarithmic growth phase cells of the above five bacteria , rinsed with saline, dried and refrigerated for later use. Among them, five different bacteria adopt different proliferation media, among which the nutr...

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Abstract

The invention discloses a composite flora for degrading pulping and papermaking wastewater and a preparation method thereof. In the preparation method, Agrobacterium sp., Bacillus sp., Enterobacter cloacae (Enterobacterium sp.) cloacae.), Pseudomonas putida., and Pseudomonas stutzeri. were inoculated and cultured; by volume percentage, 5-8% of Agrobacterium sp. 5% Bacillus sp., 10-15% Enterobacter cloacae, 26-41% Pseudomonas putida and 34-56% Pseudomonas stutzeri .) After activation, mix and put into waste water. The invention can effectively improve the efficiency of microorganisms in degrading pulping and papermaking wastewater through the coordinated action among bacteria species.

Description

technical field [0001] The invention relates to the field of papermaking wastewater treatment, in particular to a composite bacterial group for degrading papermaking wastewater and a preparation method thereof. Background technique [0002] Biological treatment is a commonly used wastewater treatment method at present. This method decomposes and absorbs the pollutants in wastewater through the metabolism of microorganisms, so as to achieve the purpose of pollution control. Compared with other methods, biological treatment has low cost, high efficiency, easy operation, and most importantly, no secondary pollution. Therefore, it has been widely used in wastewater treatment. With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/40C12R1/38C12R1/01C02F103/28
Inventor 陈元彩窦容妮胡勇有
Owner SOUTH CHINA UNIV OF TECH
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