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Embedding microbial agent for in-situ purification of water body and preparation method thereof

A microbial agent, in-situ technology, applied in chemical instruments and methods, biological water/sewage treatment, immobilized on or in inorganic carriers, etc., can solve the problem of slow reaction start, low effective concentration of degrading bacteria, easy bacteria No loss and other problems, to achieve the effect of not easy to lose, overcome the low efficiency of sewage treatment, and simple and convenient operation

Inactive Publication Date: 2014-04-02
湖南新雨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional in-situ restoration technology of water body is to purify the water body by adding bacteria liquid or powder preparation to the water body, but there are disadvantages such as low effective concentration of degrading bacteria, easy loss of bacteria, slow reaction start, etc.

Method used

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  • Embedding microbial agent for in-situ purification of water body and preparation method thereof
  • Embedding microbial agent for in-situ purification of water body and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of microbial nutrient salt solution

[0040] Weighing mass ratio is NaCl0.4% respectively, (NH 4 ) 2 SO 4 0.2%, NaNO 3 0.1%, KH 2 PO 4 0.3% of the sample, add distilled water to 500mL, adjust the pH of the solution to 7.4.

[0041] (2) Preparation of microbial growth factor solution

[0042]Accurately weigh 50mg of vitamin B1, vitamin B6, vitamin K, nicotinic acid and thiamine samples respectively, dissolve them in 25% ethanol solution and set the volume to 500mL, and mix well to obtain vitamin B1, vitamin B6 and vitamin K, four solutions of nicotinic acid and thiamine, the above four solutions are placed in a refrigerator at 4° C. for preservation.

[0043] When in use, absorb 1.00mL of the above-mentioned four kinds of solutions, distill water to a 10mL volumetric flask, and mix well to obtain the microbial growth factor solution. .

[0044] (3) Preparation of microbial liquid for sewage treatment and addition of enzyme preparations

[0045] ...

Embodiment 2

[0051] (1) Preparation of microbial nutrient salt solution

[0052] Weighing mass ratio is NaCl0.3% respectively, (NH 4 ) 2 SO 4 0.2%, NaNO 3 0.2%, KH 2 PO 4 0.3% of the sample, add distilled water to 500ML, adjust the pH of the solution to 7.2.

[0053] (2) Preparation of microbial growth factor solution

[0054] Accurately weigh 40mg of vitamin B1, vitamin B6, vitamin K, nicotinic acid and thiamine samples respectively, dissolve them in 25% ethanol solution and set the volume to 500mL, and mix well to obtain vitamin B1, vitamin B6 and vitamin K, four solutions of nicotinic acid and thiamine, the above four solutions are placed in a refrigerator at 4° C. for preservation.

[0055] Immediately before use, draw 1.00mL of the above-mentioned four solutions respectively, and distilled water to a 10mL volumetric flask, and mix well to obtain the microbial growth factor solution.

[0056] (3) Preparation of microbial liquid for sewage treatment and addition of enzyme prepar...

Embodiment 3

[0063] (1) Preparation of microbial nutrient salt solution

[0064] Weighing mass ratio is NaCl0.4% respectively, (NH 4 ) 2 SO 4 0.2%, NaNO 3 0.1%, KH 2 PO 4 0.3% of the sample, add distilled water to 500mL, adjust the pH of the solution to 7.3.

[0065] (2) Preparation of microbial growth factor solution

[0066] Accurately weigh 45mg of vitamin B1, vitamin B6, vitamin K, nicotinic acid and thiamine samples respectively, dissolve them in 25% ethanol solution and set the volume to 500mL, and mix well to obtain vitamin B1, vitamin B6 and vitamin K, four solutions of nicotinic acid and thiamine, the above four solutions are placed in a refrigerator at 4° C. for preservation.

[0067] Before use, draw 1.00mL of the above-mentioned four kinds of solutions respectively, distilled water to volume and place in a 10mL volumetric flask, and then mix well to obtain the microbial growth factor solution.

[0068] (3) Preparation of microbial liquid for sewage treatment and additio...

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Abstract

The invention discloses a preparation method of an embedding microbial agent for in-situ purification of a water body, which compounds a plurality of microbial strains suitable for the sewage treatment with enzyme preparation, and adds nutritive salt and growth factors needed by the microbial growth in an embedding carrier so as to be more suitable for the microbial growth, so that the defects of deficient nutrition of the water body and low sewage treatment efficiency are overcome. Active carbons with strong adsorption capacity and diatomite with greater specific gravity are added in the embedding carrier; polyglutamic acid is added in the embedding carrier; the polyglutamic acid reinforces the strength of embedding particles; meanwhile, glutamic acid obtained by degrading the polyglutamic acid can be used as nutritional ingredients for the strain growth, so that preparation can be preferably colonized in a river channel without easily losing. After the embedding microbial agent is used for treating sewage for three weeks, the ammonium-nitrogen removal rate reaches 85-90%, and the COD (Chemical Oxygen Demand) removal rate reaches 90-95%.

Description

technical field [0001] The invention belongs to the field of ecological and environmental engineering, and particularly relates to an embedded microbial agent for in-situ purification of water bodies and a preparation method thereof. Background technique [0002] With the continuous development of my country's economy and society, more and more domestic sewage and industrial sewage are discharged into rivers, resulting in a decline in the water quality of rivers. According to the 2009 China Environmental Status Bulletin, among the 408 surface water monitoring sections of 203 rivers across the country, 57.3%, 24.3% and 18.4 of the sections had water quality of Grade I, III, Grade IV, and Grade V, respectively. The traditional in-situ remediation technology of water body is to purify the water body by adding bacteria liquid or powder preparation to the water body, but there are disadvantages such as low effective concentration of degrading bacteria, easy loss of bacteria, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/08C02F3/34C02F3/00
Inventor 乔长晟彭巧王羿超李雪
Owner 湖南新雨生物科技有限公司
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