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Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method

An artificial and artificial technology, applied in the field of biological control of forest pests, can solve problems such as shortage of seasonal host feeding and feed, and achieve the effects of low price, remarkable benefit and high survival rate.

Inactive Publication Date: 2015-02-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a semi-artificial feed and artificial feeding method for the trough-orbital elephant, so that it can not only continuously obtain a large number of well-developed insects with consistent physiological conditions, but also solve the problem of seasonal host feeding. shortage problem

Method used

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  • Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method
  • Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method
  • Semi-artificial feed for eucryptorrhynchus chinenis and artificial breeding method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Table 1: The weight ratio of each substance in different formulas of the semi-artificial diet for the larvae

[0047]

[0048] 4 kinds of formulas shown in table 1 are prepared into feed medium according to the following steps,

[0049] (1) Fully mix Ailanthus sinensis root powder, soybean flour, corn flour and sucrose to make a mixed powder, and at the same time dissolve sucrose, Webster's salt, ascorbic acid, paraben, potassium sorbate, soybean oil and multivitamin In water, stir and mix to make a mixed solution;

[0050] (2) Add water to the container, heat to boil, add agar, after the agar is completely melted, add the mixture, stir to dissolve;

[0051](3) Slowly add the mixed powder and stir well to mix evenly;

[0052] (4) Stop heating, divide into petri dishes while hot, compact and dry the surface moisture naturally, store at 5°C after cooling for later use, and prepare feed medium.

Embodiment 2

[0054] Select 40 chrysalis pupae, and 37 eclosion adults under the conditions of temperature 25-28°C and humidity 70-80%. Pair the adult moths with a male-to-female ratio of 1:1, put them into a plastic box with a diameter of 12 cm, and feed them on the branches and leaves of Ailanthus ailanthii for 10 days, and then put in sliced ​​apples with a thickness of 1 cm. A pair of adults per box, 3 ~ 7 days after laying eggs, 380 eggs are laid, and the egg period is 10 days. After routine disinfection, they were placed at 25°C, and 345 larvae were hatched.

[0055] Configure the semi-artificial feed according to formula 1, pack it into plastic petri dishes, insert the newly hatched larvae (each container inserts 1 head), put them into a constant temperature incubator, and place them in the dark for rearing. Depending on the feeding situation, the feed was changed at regular intervals. After 2 weeks, 200 larvae survived, and 144 larvae pupated after 103 days. The pupae were transfe...

Embodiment 3

[0057] Select 60 chrysalis pupae, and 45 eclosion adults under the conditions of temperature 25-28°C and humidity 65-85%. Put the adult moths into a plastic box with a diameter of 12 cm according to the ratio of male to female in a ratio of 1:1, and feed them on the branches and leaves of Ailanthus ailanthii for 10 days, and then put in sliced ​​apples with a thickness of 1 cm, a pair of adults per box, After 3 to 7 days, eggs were laid, 460 eggs were laid, and the egg period was 11 days. Routine disinfection (first sterilized with 75% alcohol for 10 seconds, and then rinsed twice with sterile water) was placed at 28°C, and 403 larvae were hatched. .

[0058] Configure the semi-artificial feed according to formula 2, pack it into plastic petri dishes, insert the newly hatched larvae (each container inserts 1 head), put them into a constant temperature incubator, and place them in the dark for rearing. Depending on the feeding situation, the feed was changed at regular interva...

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Abstract

The invention provides a semi-artificial feed for eucryptorrhynchus chinenis and an artificial breeding method and belongs to the technical field of biological prevention and treatment on forestry pests. The artificial breeding method comprises the steps of (1) performing female and male pairing on adult insects, respectively putting the paired pests into a container for mating oviposition, feeding the adult insects with ailanthus leaves, and putting apple slices into the container, wherein the apple slices are used for feeding the adult insects, and a place is supplied to oviposition of the adult insects; (2) incubating young insects; and (3) mixing the semi-artificial feed to prepare a feed culture medium provided with orifices, inoculating the young insects obtained in the step (2) into the orifices, and breeding the insects under a dark condition until the young insects are pupated. The semi-artificial feed comprises ailanthus root powder, soybean powder, corn powder, saccharose, agar, Webster salt, nipagin, potassium sorbate, soybean oil and water. According to the semi-artificial feed and the artificial breeding method, a large number of insects which grow consistently and have consistent physiological statuses can be continuously obtained; furthermore, the problem of shortage of seasonal host breeding feeds can be solved.

Description

[0001] This invention is carried out under the support of the 12th Five-Year Science and Technology Support Program Project (2012BAD19B07) and the National Forestry Public Welfare Industry Research Project (201204501). technical field [0002] The invention belongs to the technical field of biological control of forestry pests, and in particular relates to a semi-artificial feed and an artificial feeding method for sulcus-orbital elephants. Background technique [0003] Eucryptorrhynchus chinensis (Olivier), commonly known as stink elephant, belongs to the genus Eucryptorrhynchus chinensis of Coleoptera. Ailanthus altissima (Mill.) Swingle and its variant Ailanthus altissima var. Qiantouchun are the main pests, distributed in Shaanxi, Beijing, Henan, Hebei, Shandong, Shanxi, Liaoning, Heilongjiang, Shanghai, Jiangsu , Sichuan, Ningxia and other provinces. In 2003, the Orbital Elephant was included in the list of "dangerous forestry pests" by the State Forestry Administratio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K1/18A23K1/14
Inventor 温俊宝杨鹏焦建锋张艳刘振凯
Owner BEIJING FORESTRY UNIVERSITY
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