Recombination protein for detection of virus of porcine reproductive and respiratory syndrome and preparation method and application thereof

Abstract

The invention discloses a recombination protein for detection of virus of porcine reproductive and respiratory syndrome, which is a fusion gene code spliced by a single-chain antibody gene resisting surface H antigen of human red blood cell and a single-chain antibody gene resisting the virus of porcine reproductive and respiratory syndrome. The recombination protein has dual functions of being capable of both combining with human red blood cell without agglutinating and combining with the virus of porcine reproductive and respiratory syndrome, and has observable agglutination phenomenon under action of virus bridging. The recombination protein prepared by the method can be used for detecting virus of porcine reproductive and respiratory syndrome, is simple to operate, has high sensitivity and strong specificity, and meets the requirement of base site on rapidness and simpleness and convenience.

Description

technical field [0001] The invention belongs to the technical field of recombinant proteins, and in particular relates to a recombinant protein for detection of porcine reproductive and respiratory syndrome virus and its preparation method and application. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as "pig blue ear disease", is an acute, highly contagious infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). Clinically, it is mainly manifested as late abortion, premature birth, stillbirth, mummified fetus and other reproductive disorders in pregnant sows and severe respiratory diseases in pigs of various ages, especially piglets. The disease first occurred in the United States in 1987, and has since occurred in major pig-raising countries. It is now distributed worldwide, causing serious harm to the pig industry. PRRSV was isolated for the first time in my country in 1996, and it...

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Problems solved by technology

Current PRRSV detection techniques include virus isolation and identification, virus neutralization test, immunoperoxidase monolayer test, indirect immunofluorescence antibody test, immunohistochemistry, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, Real-time fluorescent quantitative reverse transcription-polymerase chain reaction, in situ hybridization technology, gene chip technology, reverse transcription-loop-mediated isothermal amplification technology, etc., but these detection methods have high requirements and require special instruments and professional skills personnel, the cost of detection is expensive, and it takes a relatively long time, which greatly limits its application in grassroots units

Our country is still dominated by small and medium-sized pig farms in rural areas. Due to the limitations of economic and technical conditions, these pig farms cannot establish their own testing laboratories. PRRSV detection reagents and methods for grassroots and field use

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