Extraction process of shark chondroitin

A shark chondroitin and extraction process technology, applied in the field of natural product extraction, can solve the problems of low extraction yield, low product purity, high labor intensity, etc., and achieve the effects of high yield and good product purity

Active Publication Date: 2014-04-16
南通市通州区五接农业机电管理站有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction method usually uses shark cartilage powder as raw material, and it is obtained through alcohol precipitation after water extraction. The extraction yield of the traditional method is low, the labor intensity is high, and the product purity is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1. Take fresh shark cartilage, wash it, crush it into fine powder, and extract it in a NaOH solution with a mass concentration of 1% to 3% at a temperature of 30°C to 40°C to obtain an extract;

[0025] Step 2: Use dilute sulfuric acid to adjust the pH of the extract to 7.5, then add trypsin in an amount of 0.5% of the weight of the extract, heat to 35°C, and perform enzymolysis to obtain an enzymolysis solution;

[0026] Step 3, after deactivating the enzyme from the enzymatic hydrolysis solution, filter it with an ultrafiltration membrane to obtain an ultrafiltration permeate, and then filter it with a nanofiltration membrane to obtain a concentrated solution;

[0027] Step 4: Spray-dry the concentrated solution into powder, send the powder into the extraction kettle, raise the temperature to 40°C, and pass the entrainer and CO 2 The mixed fluid after the gas is mixed, the entrainer is a mixed liquid prepared by water and ethanol at a volume ratio of 2:1, the wei...

Embodiment 2

[0032] Step 1. Take fresh shark cartilage, wash it, crush it into fine powder, and extract it in a NaOH solution with a mass concentration of 1% to 3% at a temperature of 30°C to 40°C to obtain an extract;

[0033] Step 2: Use dilute sulfuric acid to adjust the pH of the extract to 8.5, then add trypsin in an amount of 1% of the weight of the extract, heat to 45°C, and perform enzymolysis to obtain an enzymolysis solution;

[0034] Step 3, after deactivating the enzyme from the enzymatic hydrolysis solution, filter it with an ultrafiltration membrane to obtain an ultrafiltration permeate, and then filter it with a nanofiltration membrane to obtain a concentrated solution;

[0035] Step 4: Spray-dry the concentrated solution into powder, send the powder into the extraction kettle, raise the temperature to 50°C, and pass the entrainer and CO 2 The mixed fluid after the gas is mixed, the entrainer is a mixed liquid prepared by water and ethanol at a volume ratio of 3:1, the weigh...

Embodiment 3

[0040] Step 1. Take fresh shark cartilage, wash it, crush it into fine powder, and extract it in a NaOH solution with a mass concentration of 1% to 3% at a temperature of 30°C to 40°C to obtain an extract;

[0041] Step 2: Use dilute sulfuric acid to adjust the pH of the extract to 8.0, then add trypsin in an amount of 0.6% of the weight of the extract, heat to 40°C, and perform enzymolysis to obtain an enzymolysis solution;

[0042] Step 3, after deactivating the enzyme from the enzymatic hydrolysis solution, filter it with an ultrafiltration membrane to obtain an ultrafiltration permeate, and then filter it with a nanofiltration membrane to obtain a concentrated solution;

[0043] Step 4: Spray-dry the concentrated solution into powder, send the powder into the extraction kettle, raise the temperature to 46°C, and pass the entrainer and CO 2 The mixed fluid after the gas is mixed, the entrainer is a mixed liquid prepared by water and ethanol at a volume ratio of 2:1, the wei...

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PUM

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Abstract

The invention relates to an extraction method of chondroitin, particularly to an extraction process of shark chondroitin, and belongs to the technical field of natural product extraction. According to the extraction process, an enzymic method, membrane separation and carbon dioxide supercritical extraction are integrated. The extraction process comprises the steps as follows: taking fresh shark cartilage, washing the shark cartilage clean, crushing the shark cartilage into fine powder, and extracting the fine powder in a NaOH solution to obtain an extract liquid; adjusting the pH of the extract liquid, and then adding trypsin for enzymolysis to obtain enzymatic hydrolysate; performing enzyme deactivation on the enzymatic hydrolysate, filtering the enzymatic hydrolysate with an ultra-filtration membrane to obtain an ultra-filtration permeate, and filtering the permeate with a nanofiltration membrane to obtain a concentrated solution; and spray-drying the concentrated solution into powder, feeding the powder into an extraction kettle for carbon dioxide supercritical extraction to obtain chondroitin solids. An integrated extraction process of the shark chondroitin is adopted, and the extraction process has the characteristics of high yield and high product purity.

Description

[0001] technical field [0002] The invention relates to a method for extracting chondroitin, in particular to an extraction process of shark chondroitin which integrates enzyme method, membrane separation and carbon dioxide supercritical extraction, and belongs to the technical field of natural product extraction. [0003] Background technique [0004] Chondroitin (chondroitin) generally has a molecular weight of 20,000 to 50,000. It is a white powder with strong water absorption and is easily soluble in water and becomes a viscous solution. Its chemical composition is D-glucuronic acid and acetylhexosamine (glucose and semi Lactose) is a high polymer linked by 1,3-b-glycosidic bonds. Its main functions are: 1. Activate the production of antibodies in the body, activate lymphatic macrophages, enhance the body's immunity, and delay aging. 2. Reduce joint inflammation and relieve pain, such as osteoarthritis, rheumatoid arthritis, hyperosteogeny, lumbar disc herniation, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCY02P20/54
Inventor 袁宏亮
Owner 南通市通州区五接农业机电管理站有限公司
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