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Phosphate-dissolving fungus and application of the same in biological phosphorus fertilizer preparation and mushroom cultivation

A technology of phosphorus-solubilizing fungi and microbial strains, applied in biochemical equipment and methods, preparation of organic fertilizers, and methods based on microorganisms, can solve the problems of large nutrient loss and long time consumption, so as to accelerate straw maturity and promote growth , the effect of huge development potential

Inactive Publication Date: 2014-04-30
山东泰宝生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the natural fermentation method of composting is the main method for decomposing dung and grass, which takes a long time and causes a large loss of nutrients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment one: the cultivation of Aspergillus niger MZ bacterial strain

[0032] Cultivate and compare in five groups, inoculate the thalli of Aspergillus niger MZ strain into PDA medium (200g of peeled potatoes and chop, boil in water for 30 minutes, filter and get the filtrate, 20g of sucrose, 20g of agar, replenish water to 1000 ml, pH natural) slant, cultured at 20°C, 22°C, 24°C, 26°C, 28°C, 30°C respectively, and obtained slant seeds after 4-10 days successively.

[0033] By comparison, the most suitable culture condition was cultured at 28°C for 4 days.

Embodiment 2

[0034] Embodiment two: the preparation of MZ decomposing agent

[0035] The Aspergillus niger MZ strain was cultured on PDA solid medium at 28°C for 4 days, the hyphae and spores on the surface of the plate were scraped off with a sterile toothpick and inoculated into cottonseed hull medium, and cultured at 28°C, the experiment showed that After 5-8 days, MZ decomposing agent can be obtained.

[0036] Through experimental comparison, the most suitable culture condition is 28°C for 4 days.

[0037] The cottonseed hull culture medium is prepared by adding PDA liquid culture medium to cottonseed hulls and mixing, and the water content is 35-45%.

Embodiment 3

[0038] Example 3: Using MZ decomposing agent to ferment crop straw to prepare biological phosphate fertilizer

[0039] Crop stalks such as rice, wheat, corn, etc. are naturally air-dried, crushed into fragments smaller than 3 cm, and then mixed with grass carbon at a weight ratio of 7:3, and then mixed at a ratio of 0.1-5% of the dry weight of the pile. Inoculate MZ decomposing agent, mix well and adjust the moisture content of the stack to 30%-45%, stack the stack indoors in a trapezoidal shape, the bottom of the stack is 1-1.5 meters long, and the top bottom is 0.5-1 meters long. The height is 0.5-1 meter, the pile is turned every 3 days, the fermentation is terminated after 18-21 days of fermentation, the fermentation product is dried below 65°C or at room temperature until the moisture content is less than 5%, crushed and sieved to obtain biological Phosphate fertilizer.

[0040] After testing, the biological phosphate fertilizer prepared by this method generally has a ...

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Abstract

The invention relates to phosphate-dissolving fungus and preparation of a decomposition agent by using the phosphate-dissolving fungus, and belongs to the technical field of agricultural waste resource comprehensive utilization, wherein the prepared decomposition agent can be used for biological phosphorus fertilizer prodution and mushroom cultivation. According to the present invention, the microorganism is a AspergillusnigerMZ strain, the strain is preserved in the China general microbiological culture collection center on April 12, 2013 and has the preservation number of CGMCC No.7464, and the ITS4 gene sequence (the sequence number of the ITS4 gene sequence in the GeneBank database is HQ850370) of the MZ strain is the main feature basis for identifying the strain; with the decomposition agent prepared by using the MZ strain, straws of corn, rice, wheat and other crops can be subjected to complete decomposition within 21 days through a composting fermentation method so as to convert into the biological phosphorus fertilizer; and the decomposition agent prepared by using the MZ strain can further be used for rapid decomposition of agaricus bisporus, volvariella volvacea and other cultivation materials and increase of mushroom yield.

Description

technical field [0001] The invention relates to a method for preparing a straw decomposing agent by using a phosphorus-dissolving fungus, and using the decomposing agent to ferment straw-like agricultural waste to prepare biological phosphate fertilizer and cultivated mushrooms, belonging to the technical field of microbial bacterial agents. Background technique [0002] Straw returning to the field is beneficial to improving soil physical, chemical and biological properties, fertilizing the soil, and promoting crop growth and yield. Crop straw is mainly composed of cellulose, hemicellulose, lignin, waxy substances and a small amount of ash. The crystal structure of cellulose is filled with hemicellulose and lignin macromolecules with a three-dimensional network structure. Combined together in a specific way to form a stable complex structure. Although cellulose and hemicellulose polysaccharide organic substances are easily decomposed by microorganisms or enzymes, lignin a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C05F17/00C12R1/685
CPCY02W30/40
Inventor 周峰莫明和杨舒惠
Owner 山东泰宝生物科技股份有限公司
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