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Preparation method of folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material

A rare earth doping, nanomaterial technology, applied in luminescent materials, material excitation analysis, chemical instruments and methods, etc., can solve the problems of lack of targeting, poor solubility, strong toxicity, etc., achieving low cost, low synthesis temperature, Simple process effect

Inactive Publication Date: 2014-05-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Before, we published the patent "Preparation method of water-soluble red and green light tunable rare earth doped up-conversion nanomaterials" (patent application number 201310403495.8, publication number CN103436263A) adopts ethylene glycol-high-purity water system, and the synthesized particles have uniform size, adjustable color, and good water solubility, but show certain defects in the later stage of cell imaging, such as poor solubility (up to 10 mg / mL) , weak fluorescence, strong toxicity and no targeting

Method used

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  • Preparation method of folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material
  • Preparation method of folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material
  • Preparation method of folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First, the yttrium oxide Y 2 o 3 4mmol, ytterbium oxide Yb 2 o 3 0.9mmol, erbium oxide Er 2 o 3 0.1mmol (Y / Yb / Er=80 / 18 / 2), weigh the mass, put it into a 50mL small beaker, add 10mL concentrated nitric acid, 5mL high-purity water, nitrate in a fume hood at 100oC, evaporate to dryness, add about 20mL high-purity water , re-dissolve the rare earth nitrate salt, transfer it to a 50mL volumetric flask, shake it to a constant volume, and take 5mL stock solution each time for experiments.

Embodiment 2

[0032]Weigh 16mmol of NaF, put it in a 50mL beaker, add 8mL diethylene glycol and 2mL high-purity water, 0.15gPEI, heat and stir at 20oC, pipette 5mL stock solution with a 5mL pipette, add dropwise to the above solution, and transfer to a three-necked flask In the middle, pass nitrogen gas, and react in an oil bath at 200oC for 0.5h. Transfer to a 25mL reaction kettle, react at 200oC for 24h, cool naturally, measure the pH to 8, transfer to a 5mL centrifuge tube, centrifuge at 10000rpm, 10oC for 5min, pour off the above liquid, add 3mL95% (w / w) Ethanol, sonicate for 15min, continue to centrifuge, then wash with 3mL 95% (w / w) ethanol and 3mL high-purity water three times, transfer to a watch glass, and dry under vacuum at 50oC.

Embodiment 3

[0034] Disperse the centrifuged and washed particles (PEI-UCNPs) in Example 2 directly in high-purity water, weigh 0.5g of folic acid (FA), add 0.01M sodium hydroxide (NaOH) to dissolve it, and shake for 15 minutes, according to Example 3 After centrifugal washing and drying, folic acid self-assembled upconverting nanoparticles (FA-UCNPs) were obtained.

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Abstract

The invention discloses a preparation method of a folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material. The preparation method mainly comprises the following steps: taking 9mL of diglycol and 6mL of high-purity water as solvents, adding 1mmol of Ln(Y / Yb / Er=80 / 18 / 2), 16mmol of NaF and 0.15g of polyethyleneimine (PEI), measuring the pH which is 9, stirring and reacting for 0.5 hour in the presence of nitrogen at 200 DEG C, transferring to a reaction kettle, reacting for 24 hours at 200 DEG C, naturally cooling and centrifugally washing to obtain polyethyleneimine modified up-converted nanometer particles (PEI-UCNPs), dispersing 1g of the PEI-UCNPs in the high-purity water, adding 0.1g of folic acid, oscillating for 15 minutes, centrifugally washing, and then dispersing in the high-purity water to obtain folic acid self-assembled up-converted nanometer particles (FA-UCNPs). As the folic acid and a folic acid receptor can be specifically bound with each other, the folic acid self-assembled rare-earth doped up-converted nanometer material can be used for identifying cancer cells and applied to targeting imaging of the cancer cells.

Description

[0001] This patent is supported by the General Project 21375095 of the National Natural Science Foundation of China, the National Excellent Doctoral Dissertation Author Special Fund FANEDD-201023, the Key Project of Tianjin Applied Basic Research Program 12JCZDJC21700, the "Young and Middle-aged Backbone Innovative Talents Training Program" ZX110GG015 of Tianjin Universities and Tianjin Supported by the "Bohai Scholars" Project of Talent Introduction 5RL098 of Normal University. technical field [0002] The invention belongs to the field of preparation of fluorescent nanomaterials, in particular to a preparation method of folic acid self-assembled water-soluble rare earth doped up-conversion nanomaterials, which is applied to cell imaging. Background technique [0003] Rare earth-doped upconversion nanomaterials have many advantages that organic dyes and semiconductor quantum dots do not have, including long fluorescence lifetime without flickering, low toxicity, high fluores...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/06G01N21/64
Inventor 李妍胡悦立孙玉绣陈晓芳张庆鑫
Owner TIANJIN NORMAL UNIVERSITY
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