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Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

A technology for photobioreactor and microalgae cultivation, which is applied in the field of cylindrical air-lift high-efficiency photobioreactors, and can solve the complex operation of commercial reactor technology, the inability to adjust light intensity and quality, and the need to ignore light, etc. problems, to achieve the effect of improving photosynthesis efficiency, simple installation and operation, and preventing biological pollution

Active Publication Date: 2014-05-07
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there may still be some deficiencies in the closed reactors developed and applied at present. For example, some commercial reactors have complicated operation and high investment costs, and some commercial reactors and self-developed reactors may ignore the cultivation of microalgae. The demand for light in the process, such as the light transmission supply problem when the algae cell density increases, cannot adjust the appropriate light intensity and light quality for specific microalgae species, etc.

Method used

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  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof
  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof
  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A cylindrical air-lift photobioreactor for microalgae cultivation, comprising a light-transmitting culture tank 1, characterized in that: it also includes an illumination control device 2 arranged outside the light-transmitting culture tank 1, arranged on the light-transmitting culture The temperature control device 3 inside the tank 1 and the gas supply device 4 arranged at the bottom of the light-transmitting culture tank 1, the top of the light-transmitting culture tank 1 is provided with an inoculation sample inlet 6 and a gas outlet 7, and the gas outlet is provided with a second filter device 26, the bottom of the light-transmitting tank body 1 is provided with an algae liquid collection port 11, and the algae liquid collection port 11 is provided with a valve 27, and the temperature control device 3 includes a temperature probe 8, a quartz glass temperature control rod 12, a temperature controller 13 and Jacket 9, the jacket 9 extends from the top of the light-tra...

Embodiment 2

[0061] Using the cylindrical airlift photobioreactor described in Example 1 to apply to the photosynthetic high-density autotrophic cultivation of microalgae, taking the model cyanobacterium Synechocystis sp.PCC6803 as an example, the details are as follows:

[0062] 1. Sterilization of medium and light-transmitting culture tanks. The autotrophic medium adopts the conventional BG11 medium, and the BG11 medium is added into the light-transmitting culture tank. The height of the medium is 80% of the height of the tank. Remove from the jacket.

[0063] 2. Installation of cylindrical airlift photobioreactor. Install light-emitting diode groups on the inner wall of the lighting control device, the light quality is white light, and the light intensities of the six groups of light-emitting diode groups are 15, 15, 15, 15, 30 and 30 μmol photons m -2 the s -1 , through the combination of LED groups can provide a light intensity range of 30-120μmol photons m -2 the s -1 4 levels o...

Embodiment 3

[0070] Using the cylindrical airlift photobioreactor described in Example 1 to apply to the photosynthetic high-density autotrophic culture of microalgae, Chlorella vulgaris (from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, No. FACHB-1068) was used as For example, the details are as follows:

[0071] 1. Sterilization of medium and light-transmitting culture tanks. The autotrophic medium adopts the conventional BG11 medium, and the BG11 medium is added into the light-transmitting culture tank. The height of the medium is 70% of the height of the tank. Remove from the jacket.

[0072] 2. Installation of cylindrical airlift photobioreactor. Install light-emitting diode groups on the inner wall of the housing of the lighting control device, the light quality is red light, and the light intensity of the six groups of light-emitting diode groups is 40 μmol photons m -2 the s -1 , through the combination of LED groups can provide a light intensity range ...

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Abstract

The invention discloses a cylindrical air-lift type high-efficient photobioreactor for microalgae culture and an application thereof. The cylindrical air-lift type high-efficient photobioreactor comprises a light transmission culture tank, an illumination control device, a temperature control device and a gas supply device, wherein the illumination control device is arranged outside the light transmission culture tank, the temperature control device is arranged inside the light transmission culture tank, the gas supply device is arranged at the bottom of the light transmission culture tank, an inoculation sample injection hole and an air outlet are formed in the top of the light transmission culture tank, a second filter is arranged at the air outlet, an algae liquid collecting hole is formed in the bottom of a light transmission culture tank body, and a valve is arranged at the algae liquid collecting hole. The cylindrical air-lift type high-efficient photobioreactor has the advantages that the cylindrical tank body with a large proportion of the height to the diameter is adopted, the illumination control device with replaceable adjustable light intensity and light quality is arranged, the conditions of the self shielding phenomenon of algae cells generated after the increase of microalgae culture density and the requirements of specific algae varieties on different light quality are solved, the sufficient luminous energy supply is ensured, the photosynthetic efficiency of microalgae is improved, and the high-density culture is easy to realize.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a cylindrical air-lift high-efficiency photobioreactor for microalgae cultivation and its application. The reactor is used for targeted microalgae photosynthetic high-density autotrophic cultivation. Background technique [0002] Microalgae are prokaryotic or eukaryotic single-celled photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO 2 Accumulated biomass can convert light energy into chemical energy and store it in the cell in the form of organic matter such as oil or starch. As the oldest low-level photosynthetic organisms, microalgae can directly use sunlight, CO 2 And simple nutrients such as N and P grow rapidly and synthesize various high-value active substances such as oil (mainly triglyceride), protein, polysaccharide and carotenoid in the cell. The...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/04C12N1/12C12R1/89
CPCC12M21/02C12M23/08C12M29/04C12M31/10C12M41/06C12M41/12C12N1/12
Inventor 王强陈辉何晨柳
Owner INST OF AQUATIC LIFE ACAD SINICA
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