Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof
A technology for photobioreactor and microalgae cultivation, which is applied in the field of cylindrical air-lift high-efficiency photobioreactors, and can solve the complex operation of commercial reactor technology, the inability to adjust light intensity and quality, and the need to ignore light, etc. problems, to achieve the effect of improving photosynthesis efficiency, simple installation and operation, and preventing biological pollution
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Embodiment 1
[0049] A cylindrical air-lift photobioreactor for microalgae cultivation, comprising a light-transmitting culture tank 1, characterized in that: it also includes an illumination control device 2 arranged outside the light-transmitting culture tank 1, arranged on the light-transmitting culture The temperature control device 3 inside the tank 1 and the gas supply device 4 arranged at the bottom of the light-transmitting culture tank 1, the top of the light-transmitting culture tank 1 is provided with an inoculation sample inlet 6 and a gas outlet 7, and the gas outlet is provided with a second filter device 26, the bottom of the light-transmitting tank body 1 is provided with an algae liquid collection port 11, and the algae liquid collection port 11 is provided with a valve 27, and the temperature control device 3 includes a temperature probe 8, a quartz glass temperature control rod 12, a temperature controller 13 and Jacket 9, the jacket 9 extends from the top of the light-tra...
Embodiment 2
[0061] Using the cylindrical airlift photobioreactor described in Example 1 to apply to the photosynthetic high-density autotrophic cultivation of microalgae, taking the model cyanobacterium Synechocystis sp.PCC6803 as an example, the details are as follows:
[0062] 1. Sterilization of medium and light-transmitting culture tanks. The autotrophic medium adopts the conventional BG11 medium, and the BG11 medium is added into the light-transmitting culture tank. The height of the medium is 80% of the height of the tank. Remove from the jacket.
[0063] 2. Installation of cylindrical airlift photobioreactor. Install light-emitting diode groups on the inner wall of the lighting control device, the light quality is white light, and the light intensities of the six groups of light-emitting diode groups are 15, 15, 15, 15, 30 and 30 μmol photons m -2 the s -1 , through the combination of LED groups can provide a light intensity range of 30-120μmol photons m -2 the s -1 4 levels o...
Embodiment 3
[0070] Using the cylindrical airlift photobioreactor described in Example 1 to apply to the photosynthetic high-density autotrophic culture of microalgae, Chlorella vulgaris (from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, No. FACHB-1068) was used as For example, the details are as follows:
[0071] 1. Sterilization of medium and light-transmitting culture tanks. The autotrophic medium adopts the conventional BG11 medium, and the BG11 medium is added into the light-transmitting culture tank. The height of the medium is 70% of the height of the tank. Remove from the jacket.
[0072] 2. Installation of cylindrical airlift photobioreactor. Install light-emitting diode groups on the inner wall of the housing of the lighting control device, the light quality is red light, and the light intensity of the six groups of light-emitting diode groups is 40 μmol photons m -2 the s -1 , through the combination of LED groups can provide a light intensity range ...
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