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Application of Tibetan medicine duhat fruit tannin monomer

A kind of technology of tannin and use, applied in the field of tannin monomer

Inactive Publication Date: 2014-05-28
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no use of the tannin monomer gallic acid, methyl gallate, 3,4,8,9,10-pentahydroxydibenzo[b,d]pyran-6-one in the preparation of monoamine oxidase inhibitors

Method used

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  • Application of Tibetan medicine duhat fruit tannin monomer
  • Application of Tibetan medicine duhat fruit tannin monomer
  • Application of Tibetan medicine duhat fruit tannin monomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of gallic acid, methyl gallate, 3,4,8,9,10-pentahydroxydibenzo[b,d]pyran-6-one

[0029] Grind dried sage (5kg), extract with 95% industrial ethanol, reflux extraction twice, each time for 2 hours, suction filter, concentrate under reduced pressure to the extract, recover ethanol, then suspend the extract in 5L distilled water, and use 4 mol / L NaOH

[0030] Adjust the suspended aqueous solution to pH = 10-11, let stand overnight; filter with suction; then use 2 mol / L HCl solution to adjust pH = 2-3, extract with ethyl acetate in equal volume, concentrate under reduced pressure, and recover ethyl acetate to obtain Sa Zhe total organic acids. Perform polyamide (100-200 mesh) column chromatography on the total organic acids of Zazhe, elution with water, 30%, 50%, 70%, 90% methanol gradient in turn, discard the water-eluted part, 30%-90% The eluted fractions were tracked and merged by TLC to obtain 6 fractions (Fr.1-6). The monoamine oxidase inhibito...

Embodiment 2

[0031] Example 2 Determination of the monoamine oxidase inhibitory activity of the tannin monomer components by enzyme labeling method

[0032] Determination of Sazitannin Monomer Components by Enzyme Labeling Method: Compounds 1 - 3(concentration is 50 μ g / ml), the inhibition rate of iproniazid phosphate is the positive control drug. The specific experimental process is as follows:

[0033] (1) Preparation of monoamine oxidase:

[0034] One female wistar rat was killed by cervical dislocation, the liver was taken out, cut into pieces, washed several times with phosphate buffer (pH=7.6), after washing, 20ml of 0.3M sucrose buffer was added for homogenization After fully homogenizing, take 10ml each and pour into two 50ml centrifuge tubes. After washing the homogenizer with 20ml of 0.3M sucrose buffer, 10ml of each was poured into the aforementioned centrifuge tubes. After balancing with a tray balance, centrifuge at 1000×g for 10 min at -4°C. Aspirate the supernatant, ba...

Embodiment 3

[0041] Example 3 Forced swimming test (depressed mouse model)

[0042] Put male SD rats individually into a glass cylinder with a water depth of 15cm (height: 40cm and diameter: 18cm), the water temperature is (24±2)°C, and the water depth can be slightly adjusted so that the rear feet of the rats can just touch the bottom of the cylinder but are not enough to support Body is appropriate. When first put in, the rats were forced to swim and struggled to climb up or dive into the bottom of the cylinder. After 2-3 minutes, the activity gradually weakened, and the intermittent immobility state appeared, and the time became longer and longer. About 80% of the total time, the rats were dried after 15 minutes of the pre-test. Experimental grouping: excluding the unqualified ones in the preliminary test, 50 qualified rats were randomly divided into 5 groups, 10 rats in each group. The blank control group and the fluoxetine positive control group were intragastrically given normal sal...

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PUM

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Abstract

The invention relates to application of a Tibetan medicine duhat fruit tannin monomer, and in particular relates to application of a duhat fruit tannin monomer consisting of gallic acid, methyl gallate and 3,4,8,9,10-pentahydroxydibenzo[b,d]pyran-6-one in preparing a monoamine oxidase inhibitor. The application is characterized in that gallic acid, methyl gallate and 3,4,8,9,10-pentahydroxydibenzo[b,d]pyran-6-one are applied to preparation of medicines for treating depression and Parkinson disease.

Description

technical field [0001] The present invention relates to the use of tannin monomers. Background technique [0002] With the rapid development of the global economy and the aggravation of population aging, modern people's life pressure is increasing, and there are more and more patients with depression and Parkinson's disease. Therefore, the study of drugs for the treatment of depression and Parkinson's disease (monoamine oxidase inhibitor drugs) has aroused widespread interest. [0003] Sazhe is a commonly used Tibetan medicine. It is mainly used for the treatment of kidney disease and three disasters in Tibetan areas of my country. Its base plant is Hainan rose fruit Syzygium cumini (l.) Skeels fruit. This plant is sunny, likes warm and humid climate, has strong growth suitability, and is widely distributed. It is produced in Yunnan, Fujian, Guangdong, and Guangxi; it is born in low-altitude sparse forests or wilderness (Yang Yongchang. Tibetan Medicine [M]. Xining: Qingh...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61K31/235A61K31/366A61P25/24A61P25/16
Inventor 杨中铎智康康
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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