Preparation method for fibrinolytic fermented bean curd blanks
A technology for fermented bean curd and blanks, which is applied in the field of preparation of thrombolytic fermented fermented bean curd blanks, and achieves the effects of saving production costs and growing well
- Application Information
AI Technical Summary
Problems solved by technology
 Example 1:
 The preparation of tofu slab: After soaking the selected soybeans in warm water (22℃) for 4h, refining, filtering, boiling, dotting, squatting, squeezing until the water content of the tofu slab is 70%, and after cooling treatment, cut into pieces (2.8×2.8×1.7cm) small pieces.
 Strain activation: inoculate the slant preserved Mucor strain into the PDA slant medium under aseptic operation, cultivate in a constant temperature incubator at 20°C for 72 hours, and store in the refrigerator for later use. Draw 100ul of the preserved natto bacteria into 10ml LB medium, cultivate at 37℃, 200r / min for 12h, and store in the refrigerator for later use.
 Preparation of Mucor seed solution: Add the activated Mucor slant strain to a suitable amount of sterile saline water, use a pipette to suck up the appropriate amount of the bacterial solution, inoculate it in the bran medium, incubate at 20°C for 96 hours, and put it in the Erlenmeyer flask After the...
 Example 2: Determination of enzyme activity
 Crude enzyme solution preparation: weigh the fermented bean curd in a beaker and mince it, add 2ml buffer solution to each 1g fermented bean curd in an Erlenmeyer flask, place it on a shaker at 4°C, extract for 1 hour, and take it out in a high-speed refrigerated centrifuge at 10000r / min for 10min , Take the supernatant.
 Enzyme activity determination: The fibrinolytic enzyme activity determination is based on the method of the People's Republic of China Pharmacopoeia (Part 2) and Shao Rongjun, after comprehensive improvement, the preparation of fibrin plate method. The crude enzyme solution was added to the fibrin plate, incubated in a constant temperature incubator at 37°C for 18 hours, and the diameter of the dissolved circle was measured. Take the urokinase standard curve (see attached figure 2 ) Calculate the relative enzyme activity of the sample; the protease activity is determined in accordance with GB / T2...
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