Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of quantum dot-based multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system

A carrier system and quantum dot technology, which is applied in the field of preparation of composite nano-gene carriers, can solve problems such as protein inactivation, cell damage, and reduced transfection efficiency, and achieve good biocompatibility, poor safety performance, and transfection problems. high efficiency effect

Active Publication Date: 2014-06-04
澎立生物医药技术(上海)股份有限公司
View PDF6 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The materials for preparing quantum dots usually contain toxic heavy metals, which can inactivate proteins and cause cell damage after heavy metals bind to sulfhydryl groups in mitochondrial proteins;
[0006] (2) As a gene carrier, the positively charged quantum dots on the surface can non-specifically bind to various cells in the body after entering the body, thereby reducing the transmission efficiency of siRNA and causing certain toxic and side effects to normal tissues;
[0007] (3) The existing gene carriers cannot take into account both biocompatibility and transfection efficiency at the same time, often improving the biocompatibility of quantum dots through surface modification also greatly reduces their transfection efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of quantum dot-based multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system
  • Preparation and application of quantum dot-based multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system
  • Preparation and application of quantum dot-based multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of CdTe quantum dot nanoparticles

[0030] (1) Preparation of sodium tellurium hydride: Weigh 50 mg of tellurium powder (Te) and 80 mg of sodium borohydride (molar ratio of tellurium powder and sodium borohydride = 1:5), dissolve them in 3.5 mL of ethanol, and pass through nitrogen protection. Then, 1.5 mL of deionized water was added to the solution, and reacted at 60°C for 30 min while stirring; 5.5 mL of 0.5 mol / L sulfuric acid was added to the above solution to generate hydrogen telluride gas. Absorb hydrogen telluride with 0.75mL of 0.2mol / L NaOH solution to make NaHTe solution with specific concentration.

[0031] (2) Weigh 102.3 mg of mercaptoethylamine hydrochloride (CA) and dissolve in 33 mL of deionized water, adjust the pH of the solution to 6-8 with 0.2 mol / L NaOH, then add 3 mL of 0.1 mol / L cadmium chloride solution, and stir. Under nitrogen protection, add the newly prepared NaHTe solution to the previously prepared cadmium solution (Cd), r...

Embodiment 2

[0039] 1. Preparation of CdTe quantum dot nanoparticles

[0040] (1) Preparation of sodium tellurium hydride: Weigh 50 mg of tellurium powder and 80 mg of sodium borohydride (molar ratio of tellurium powder to sodium borohydride = 1:5), dissolve them in 3.5 mL of ethanol, and pass through nitrogen protection. Then, 1.5 mL of deionized water was added to the solution, and reacted at 60°C for 30 min while stirring; 5.5 mL of 0.5 mol / L sulfuric acid was added to the above solution to generate hydrogen telluride gas. Absorb hydrogen telluride with 0.75mL of 0.2mol / L NaOH solution to make NaHTe solution with specific concentration.

[0041] (2) Weigh 147 mg of N-acetyl-L-cysteine ​​(NAC) and dissolve it in 33 mL of deionized water, adjust the pH of the solution to 8-9 with 0.2 mol / L NaOH, then add 3 mL of 0.1 mol / L cadmium chloride solution , stir. Under nitrogen protection, add the newly prepared NaHTe solution to the previously prepared cadmium solution, react for 40 minutes to...

Embodiment 3

[0049] 1. Preparation of CdTe quantum dot nanoparticles

[0050] (1) Preparation of sodium tellurium hydride: Weigh 50 mg of tellurium powder and 80 mg of sodium borohydride (molar ratio of tellurium powder to sodium borohydride = 1:5), dissolve them in 3.5 mL of ethanol, and pass through nitrogen protection. Then, 1.5 mL of deionized water was added to the solution, and reacted at 60°C for 30 min while stirring; 5.5 mL of 0.5 mol / L sulfuric acid was added to the above solution to generate hydrogen telluride gas. Absorb hydrogen telluride with 0.75mL of 0.2mol / L NaOH solution to make NaHTe solution with specific concentration.

[0051] (2) Weigh 127.503 mg of 2-dimethylaminoethanethiol hydrochloride (DMAE) and dissolve it in 33 mL of deionized water, adjust the pH of the solution to 6-8 with 0.2 mol / L NaOH, then add 3 mL of 0.1 mol / L cadmium chloride solution , stir. Under nitrogen protection, add the newly prepared NaHTe solution to the previously prepared cadmium solution,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method and application of a multifunctional nano siRNA (Small Interfering Ribonucleic Acid) carrier system which integrates tumor targeting, pH sensitivity, visualization and the like. The core of the siRNA nano carrier system consists of cadmium telluride quantum dots. A nano carrier shell consists of a tumor targeted gene, a 9 poly-l-arginine peptide chain and PEG (Polyethylene Glycol). The siRNA nano carrier is good in biocompatibility, strong in tumor targeting and high in cell transfection efficiency, and has the characteristics of high quantum yield, strong optical stability, lossless in-position real-time monitoring and the like, so that the system is suitable for being used as a nano transmission system for in vivo gene therapy of tumors.

Description

technical field [0001] The invention relates to an siRNA carrier used in gene therapy, in particular to a preparation method and application of a composite nanometer gene carrier formed of a tumor-targeting, good biocompatibility, visible and dynamic monitoring polymer biomaterial and inorganic quantum dots. Background technique [0002] RNA interference refers to post-transcriptional gene silencing due to target mRNA degradation or translational repression. Single-stranded or double-stranded RNA containing 21 to 23 bases, that is, small-molecule interfering RNA (Small-interfering RNA, siRNA), siRNA can efficiently and specifically block the expression of homologous genes in vivo through RNA interference, and promote the expression of homologous mRNAs. Degradation induces cells to exhibit the phenotype of specific gene deletion. However, naked siRNA is easily degraded by ribozyme (RNase) in vivo, has a short half-life, and low transfection efficiency. These shortcomings hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K48/00A61K47/48
Inventor 朱红艳谢爱梅杨池张贝贝王逸飞
Owner 澎立生物医药技术(上海)股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com