A method for determining the content of kudzu root in a traditional Chinese medicine preparation for treating diabetes
A technology of traditional Chinese medicine preparations and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ineffective control of the quality of traditional Chinese medicine preparations for treating diabetes, affecting product production and quality assurance, and achieves high recovery rate, precision and High sensitivity, easy and quick operation
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Embodiment 1
[0026] Embodiment 1: prepare Yuquan pill
[0027] Prescription: Rehmannia: 20 parts, Ophiopogon japonicus: 15 parts, Schisandra: 5 parts, Pueraria: 21 parts, TCS: 17 parts, licorice: 6 parts.
[0028] The preparation method of Yuquan pill is as follows: the above six herbs, kudzu root, trichosanthemi and licorice are crushed into coarse powder; rehmannia glutinosa, Ophiopogon japonicus and Schisandra chinensis are decocted in water three times, the decoction is combined, filtered, the filtrate is concentrated into a thick paste, and the above coarse powder is added , stirred evenly, dried, crushed into fine powder, sieved, mixed evenly, made into pellets with water, dried, coated with medicinal charcoal, polished, and obtained.
Embodiment 2
[0029] Embodiment 2: the method for determining the content of kudzu root in the above-mentioned traditional Chinese medicine preparation for treating diabetes is determined by high performance liquid chromatography, comprising the following steps:
[0030] S1. Chromatographic conditions and system suitability experiment: octadecylsilane bonded silica gel is used as filler; methanol-water is used as mobile phase, the volume ratio of methanol to water is 14:75, and the detection wavelength is 250nm; the number of theoretical plates Calculated according to the peak of puerarin, it should not be less than 5000;
[0031] S2. Preparation of reference substance solution: accurately weigh an appropriate amount of puerarin reference substance, add 65% methanol to make a solution containing 18 μg of puerarin per 1 ml, which is the reference substance solution;
[0032] S3. Preparation of the test solution: take the product under the weight item, grind it finely, weigh 0.8g, accurately ...
Embodiment 3
[0034] Embodiment 3: The method for determining the content of kudzu root in the above-mentioned traditional Chinese medicine preparation for treating diabetes is determined by high performance liquid chromatography, comprising the following steps:
[0035] S1. Chromatographic conditions and system suitability experiment: octadecylsilane bonded silica gel is used as filler; methanol-water is used as mobile phase, the volume ratio of methanol to water is 25:85, and the detection wavelength is 250nm; the number of theoretical plates Calculated according to the peak of puerarin, it should not be less than 5000;
[0036] S2. Preparation of reference substance solution: accurately weigh an appropriate amount of puerarin reference substance, add 75% methanol to make a solution containing 22 μg of puerarin per 1 ml, which is the reference substance solution;
[0037] S3. Preparation of the test solution: take the product under the weight item, grind it finely, weigh 1.2g, accurately ...
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