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Method for high density culturing of Bacillus subtilis with high content of living bacteria

A technology for high-density cultivation of Bacillus subtilis, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as unsatisfactory biomass, extend product retention period, increase fermentation productivity, and improve product quality. efficient effect

Inactive Publication Date: 2014-06-18
重庆科迪生物有机肥有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are fewer reports about the number of viable bacteria, and the number of viable bacteria in the existing culture methods can generally only reach tens of millions (10 million). 7 individuals / mL), its biomass is not ideal, and people have been working hard to improve the number of viable bacteria.

Method used

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Examples

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Effect test

Embodiment 1

[0017] The invention relates to a method for high-density cultivation of bacillus subtilis with a high number of living bacteria. The method uses 5 L of bacillus subtilis as a strain, which is activated and inoculated into a fermentation medium for cultivation.

[0018] (1) Activation culture:

[0019] The medium used for activation is composed of the following raw materials: 250g of beef extract, 600g of peptone, 400g of glucose, 350g of sodium chloride, 550g of agar, and 47850g of distilled water.

[0020] The conditions for the activation culture are: adjust the pH value to 7.0, the temperature is 36.5° C., and activate for 20 hours.

[0021] (2) Fermentation culture:

[0022] Inoculate the activated Bacillus subtilis into the fermentation medium and cultivate:

[0023] The fermentation medium is composed of the following raw materials: 700 g of glucose, 125 g of starch, 400 g of soybean meal, 75 g of manganese sulfate, 125 g of dipotassium hydrogen phosphate, 100 g of po...

Embodiment 2

[0027] The invention relates to a method for high-density cultivation of bacillus subtilis with a high number of living bacteria. The method uses 5 L of bacillus subtilis as a strain, which is activated and inoculated into a fermentation medium for cultivation.

[0028] (1) Activation culture:

[0029] The medium used for activation is composed of the following raw materials: beef extract 150g, peptone 500g, glucose 500g, sodium chloride 250g, agar 1000g, distilled water 46600g.

[0030] The conditions of the activation culture are: adjust the pH value to 7.2, the temperature is 37° C., and activate for 24 hours.

[0031] (2) Fermentation culture:

[0032] Inoculate the activated Bacillus subtilis into the fermentation medium and cultivate:

[0033] The fermentation medium is composed of the following raw materials: glucose 800g, starch 110g, soybean meal 1500g, manganese sulfate 50g, dipotassium hydrogen phosphate 150g, potassium dihydrogen phosphate 75g, magnesium sulfate ...

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PUM

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Abstract

The invention provides a method for the high density culturing of Bacillus subtilis with a high content of living bacteria. The method comprises the following steps: activating Bacillus subtilis as a strain, inoculating to a fermentation culturing medium, and culturing. The method provides necessary conditions for the improvement of the industrialized fermentation productivity of Bacillus subtilis, improvement of the effective rate of a product and prolongation of the retention period of the product by researching the formula of the culturing medium and the culturing conditions in order to make the cell biomass of the Bacillus subtilis reach 9.2*10<8> / mL, and the cell biomass is one order of magnitude, that is, 10 times, higher than the culturing result of present common methods.

Description

technical field [0001] The invention relates to a method for cultivating bacillus subtilis with high viable count in high density. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a spore-forming G+ bacterium. The active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced during the growth of the bacterium are effective against pathogenic bacteria or endogenous bacteria. The opportunistic pathogenic bacteria of primary infection have obvious inhibitory effect; rapidly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids such as lactic acid, reduce intestinal pH, inhibit Growth of other pathogenic bacteria; stimulate the growth and development of animal immune organs, activate T and B lymphocytes, increase the level of immunoglobulin and antibody, enhance cellular immunity and humoral immunity, and improve group immunity; can synthesize vit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 李淘洪周来薪
Owner 重庆科迪生物有机肥有限公司
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