Detection kit for human herpes viruses EBV and VZV

Abstract

The invention provides a detection kit for human herpes viruses EBV and VZV. The detection kit comprises quantitative PCR reaction solution, an EBV standard, a VZV standard, an EBV positive control, a VZV positive control, a negative control, instructions and the kit body, the quantitative PCR reaction solution comprises PCR buffer, MgCl2, dNTPs, heat-resistant DNA polymerase, an EBV forward amplification primer, an EBV reverse amplification primer, a VZV forward amplification primer, a VZV reverse amplification primer, a EBV fluorescent probe and a VZV fluorescence probe. The human herpes viruses EBV and VZV can be detected in one step by using real-time quantitative PCR and two-color fluorescent probes according to the detection kit, synchronous diagnosis of EBV and VZV infections is realized, positive virus can be accurately quantified in real time, urgent need for accurate diagnosis of EBV and VZV infections in the early clinical diagnosis is met and a basis is provided for timely definitive therapy of EBV and VZV infections.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative PCR detection kit, in particular to a dual-probe real-time fluorescent quantitative PCR for simultaneously detecting Epstein-Barr virus (Epstein-Barr virus, EBV) and varicella-zoster virus (varicella-zoster virus, VZV) diagnostic kits. Background technique [0002] EBV and VZV are common human herpesviruses that cause viral encephalitis in children. After EBV invades the central nervous system, it can lead to cerebral edema, hemorrhage, deposition of immune complexes, and demyelination-like changes in brain tissue. The clinical manifestations can be acute onset or chronic active damage. Acute EBV encephalitis is mostly caused by the virus directly invading the nervous system, such as meninges, brain tissue, spinal cord, and nerve axons in multiple parts of peripheral nerves; chronic EBV encephalitis is related to autoimmune reactions, which is the de...

AI Technical Summary

Problems solved by technology

Traditional EBV and VZV detection methods mainly include virus isolation and culture and serological detection. The former was once the "gold standard" for the diagnosis of herpes virus, but it takes a long time (3-30 days) and has low sensitivity (especially in the treatment of antiviral drugs). The latter is divided into direct antigen detection method and antibody indirect detection method. The direct antigen detection method has low sensitivity because some viruses do not produce detectable antigens, and the indirect antibody detection method is due to herpes It takes about 1 week after virus infection to produce an effective concentration of antibodies, making early diagnosis impossible, and there are cross-reactions between different herpes viruses, and the specificity of antibodies is not high

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