A method, system and application of ultrasound-assisted photothermal agent-based tumor cell killing
A tumor cell, ultrasound-assisted technology, applied in the field of tumor therapy and biomedicine, can solve the problems of photothermal therapy being difficult to achieve an ideal temperature, photothermal therapy is concentration-dependent, and near-infrared light penetration is limited. Compatibility, lethality enhancement, strong penetration effect
Active Publication Date: 2018-02-27
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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Problems solved by technology
[0004] However, the penetration ability of near-infrared light in the body is limited, which makes it difficult for photothermal therapy to reach the ideal temperature in the deeper subcutaneous layer, and the therapeutic effect is not good, and photothermal reagents are concentration-dependent on photothermal therapy.
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Embodiment 1
[0135] A preparation method containing indocyanine green medicinal carrier, comprising the following steps:
[0136] 1) Dissolve PLGA in acetonitrile at a concentration of 2 mg / mL to obtain the first solution;
[0137] 2) Add 180 μg of lecithin, 120 μg of DSPE-PEG, and 750 μg of indocyanine green into 3 mL of 4% ethanol aqueous solution, in which the green indocyanine green appears insoluble and sinks to obtain the second solution;
[0138] 3) Take 1 mL of the first solution obtained in step 1) and add it dropwise to the first solution obtained in step 2). During this period, use an ultrasonic cell disruptor to sonicate for 5 minutes at a frequency of 20 kHz and a power of 130 W; the precipitate disappears and a dark green color is obtained. solution;
[0139] 4) Centrifuge and ultrafilter twice with a 10kDa ultrafiltration tube to obtain the indocyanine green-containing pharmaceutical carrier.
[0140] The particle diameter of the obtained pharmaceutical carrier containing ...
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The present invention provides an ultrasound-assisted photothermal agent-based tumor cell killing method, comprising: 1) providing a target sample, injecting a medicinal carrier containing a photothermal agent into the target sample, wherein the target sample is a tissue or Cell suspension; 2) Take the target sample treated in step 1), use an ultrasonic device to sonicate the target sample, and at the same time, use a near-infrared laser to irradiate the tumor cells until the temperature of the tumor cells exceeds 43°C, and control the output power of the ultrasound With a frequency such that the normal cell temperature does not exceed 43 °C. The combination therapy provided by the present invention combines the high efficiency of photothermal therapy with the characteristics of continuous heating and strong penetrating power of ultrasonic hyperthermia. Toxic side effects produced by normal tissues of the body. The invention also provides an ultrasound-assisted photothermal agent-based tumor cell killing system and application.
Description
technical field [0001] The invention relates to the fields of biomedicine and tumor treatment, in particular to an ultrasound-assisted photothermal agent-based tumor cell killing method, system and application. Background technique [0002] At present, surgery, radiotherapy and chemotherapy are mainly used to treat tumors clinically. These methods have relatively large toxic and side effects, and often bring great pain to patients. For example, chemotherapy drugs can cause bone marrow suppression, gastrointestinal reactions, myocardial damage, liver and kidney dysfunction, and nervous system damage. Among them, anthracyclines, alkylating agents (such as cyclophosphamide, cisplatin, etc.), antimetabolites (such as fluorouracil, capecitabine, etc.), paclitaxel can cause heart damage; bleomycin can cause pulmonary adverse reactions ; Platinum (such as oxaliplatin, cisplatin, etc.), vinblastine and paclitaxel drugs can cause adverse reactions to the nervous system. Although t...
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IPC IPC(8): A61K41/00A61P35/00
Inventor 蔡林涛罗震宇郑明彬赵鹏飞龚萍
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI