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Screw capable of absorbing compound interface and manufacturing method of screw

A technology of interface screw and screw, applied in internal bone synthesis, medical science, internal fixator, etc., can solve the problems of PLLA without osteoinductive function, unstable ligament fixation, poor osteogenic effect, etc., and achieve good osteoinductivity , good biocompatibility, and enhance the effect of promoting calcification of fibrocartilage

Inactive Publication Date: 2014-07-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]However, pure PLLA has no osteoinductive function, and the tunnel may expand due to the mismatch between the bone formation speed in the tunnel and the degree of PLLA absorption, resulting in unstable ligament fixation
Clinically, absorbable screws using composite hydroxyapatite (HAP) have improved to a certain extent in the induction of osteogenesis. However, due to the size difference between artificially synthesized HAP and HAP in human bones, the osteogenesis effect is still not good.

Method used

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  • Screw capable of absorbing compound interface and manufacturing method of screw
  • Screw capable of absorbing compound interface and manufacturing method of screw
  • Screw capable of absorbing compound interface and manufacturing method of screw

Examples

Experimental program
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Effect test

Embodiment 1

[0034] see figure 1 , figure 2 , a screw with an absorbable composite interface according to the present invention is composed of a screw cap 1, a screw rod 2, a screw head 3, a connector 4, and a gene thin layer 5, and the screw cap 1 and the screw rod 2 are fixedly connected by a connector 4, The screw head 3 is arranged on the head end of the screw rod 2, the upper end of the screw cap 1 is provided with a groove 6 corresponding to the installation screw torque wrench, the outer edge of the screw rod 2 is provided with a self-tapping thread 7, and the gene thin layer 5 covers the screw thread surface.

Embodiment 2

[0035] Example 2 Fabrication and Characterization of Absorbable Composite Interface Screws Assembled by COMP Gene TLC

[0036] (1) PLLA in 0.2g ml -1 The concentration is dissolved in 1,4-epoxyhexacycline solution, and then 20HAP powder is added, and the mass ratio of the above two is 2:8 for mixing;

[0037] (2) Add NaCl particles with a diameter of 280-450um into a screw model with a diameter of 4mm to fill it tightly, place it in an environment saturated with water vapor at 70°C for 1.5h, and after cooling at room temperature, put the PLLA / 20HAP obtained in step (1) The mixture is slowly added therein, and the air bubbles are removed by vacuuming under the condition of 0.07-0.08Mpa. Pre-freeze in -40°C refrigerator for 3 hours and then freeze for 24 hours;

[0038] (3) Soak the sample prepared in step (2) in distilled water for 24 hours, remove NaCl, and freeze-dry again to obtain PLLA / 20HAP absorbable composite interface screws;

[0039] (4) Soak the PLLA / 20HAP absorb...

Embodiment 3

[0052] Example 3 Exploration of the transfection efficiency of mesenchymal stem cells with different layers of gene thin layer.

[0053] Using the manufacturing method of Example 1, absorbable composite interface screws assembled with 0, 4, 8 and 12 layers of COMP gene thin layers were manufactured respectively. SD rat mesenchymal stem cells were cultured by density gradient centrifugation and time-difference adherence, and the mesenchymal stem cells were inoculated into a six-well plate and co-cultured with interface screws of different layers. The cell inoculation density was 40,000 cells / cm 2 . Cultured in DMEM medium plus 10% fetal bovine serum. After 48 hours, the GFP fluorescence in each group with different layers was observed with a fluorescence microscope to detect the cell transfection, and the GFP fluorescence intensity in each group was calculated by IPP6.0 software.

[0054] see Figure 6 , 7 , the results showed that the fluorescence intensity of GFP increa...

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Abstract

The invention provides a screw capable of absorbing a compound interface. The screw comprises a screw cap, a screw rod, a screw head connector, and a gene thin layer, wherein a self-taping thread is arranged on the outer edge of the screw rod; the gene thin layer covers the surface of the thread, and is an activation layer formed by a base layer of chitosan, and a hyaluronic acid layer and a pEGFP-COMP (Plasmid Enhanced Green Florescence Protein-Cartilage Oligomeric Matrix Protein) plasmid layer wrapped by lipidosome-2000, which are sequentially covered from inside to outside at an interval. The plasmids pEGFP-COMP are gradually deposited on the surface of the interface screw by using the electrostatic interaction of the chitosan, the hyaluronic acid and the lipidosome. The screw has good biocompatibility and bone inductivity, release of a COMP gene can be effectively regulated and controlled to enhance and promote the calcification fiber cartilage effect, the host cell transfection rate is increased, the bone-ligament interface is good in coalescence after the ligament is induced to be implanted, and intra-articular ligament reconstruction surgery can be combined with gene therapy. The screw is reasonable in design, simple and convenient to operate, low in cost, and applicable to industrial application, and has extremely strong feasibility.

Description

technical field [0001] The invention belongs to the manufacture of interface screws in intra-articular ligament reconstruction, in particular to an absorbable composite interface screw assembled with COMP gene thin layer and a preparation method thereof. Background technique [0002] Arthroscopic ligament reconstruction is currently an effective surgical treatment for intra-articular ligament injuries and restoration of joint stability and function. According to statistics from the American Orthopedic Sports Medicine Society (AOSSM), at least 600,000 people worldwide undergo intra-articular ligament reconstruction every year. Accounted for 21.6% of joint surgeries. Although this technique has achieved great clinical efficacy, the problem of bone-ligament interface healing still plagues the majority of clinicians. Once the ligament-bone interface is poorly healed, it is likely to lead to the failure of the entire operation. Therefore, how to improve ligament-bone healing is ...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/14A61L31/08A61L31/10A61B17/68
Inventor 严世贵吴浩波师钟丽刘安
Owner ZHEJIANG UNIV
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