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A preparation method of solid active Cordyceps mycelium separated from culture medium

A technology for Cordyceps mycelium and Cordyceps mycelium, applied in the field of health food production, can solve problems such as difficulty in separating Cordyceps mycelium and culture medium, affecting the purity and taste of Cordyceps mycelium, and increasing the production cost of Cordyceps mycelium. , to improve the efficacy of medicinal health care, to avoid low and unstable content, and to facilitate the effect of later culture.

Active Publication Date: 2016-08-31
广西华夏医药科技有限公司
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Problems solved by technology

[0005] However, currently Cordyceps mycelium is difficult to industrialized large-scale production. On the one hand, the currently disclosed methods for preparing artificial Cordyceps mycelium generally have the disadvantage of low mycelium content, and the mass ratio of mycelium to culture medium is about 1. : 61, and the content is unstable; on the other hand, it is difficult to completely separate the Cordyceps mycelium from the culture medium, and the separation process is very complicated. On the one hand, it affects the biological activity of the Cordyceps mycelium, and on the other hand, it affects the The purity and mouthfeel of body, and greatly increase the production cost of Cordyceps mycelium

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  • A preparation method of solid active Cordyceps mycelium separated from culture medium

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preparation example Construction

[0032] like figure 1 Shown, a kind of preparation method of the solid active Cordyceps mycelium that separates with substratum comprises the following steps:

[0033] Step 1, screening of Cordyceps strains. Select excellent fresh Cordyceps sinensis strains, cut out the ascus tissue of the Cordyceps strain, after external disinfection, add it to a tissue culture bottle with a small amount of sterile 0.5-1% glucose culture solution at the bottom, and conduct tissue at 22-24 degrees After 3-7 days of isolation and cultivation, the Cordyceps hyphae are obtained by separating by centrifugation and other separation methods. Inoculate Cordyceps hyphae into silkworm chrysalis, set certain culture conditions in a biological incubator, and cultivate through natural cultivation methods. After a certain temperature difference and light-stimulated cultivation, select excellent Cordyceps strains as Cordyceps strains, and put The Cordyceps strains continue to be cultivated until the spores...

Embodiment 1

[0041] Select excellent fresh Cordyceps sinensis strains, cut out the ascus tissue of the Cordyceps strain, sterilize it externally, add it to a tissue culture bottle with a small amount of sterile 1% glucose culture solution at the bottom, and conduct tissue separation and culture at 22-24 degrees After 3-7 days, the mycelium of Cordyceps sinensis is obtained by separating by means of centrifugation and the like. Select high-quality silkworm chrysalis, inoculate the mycelium of Cordyceps sinensis into the silkworm chrysalis, set certain cultivation conditions in the biological incubator, and cultivate through natural cultivation method. The cultivation temperature is 13 degrees for 2 days, 14 degrees for 1 day, and 19 degrees for 1 day. Cultivate at 20 degrees until the silkworm chrysalis is rigid and mycelia grow out of the silkworm chrysalis, and the silkworm chrysalis that grow mycelia are subjected to a gradient temperature change stimulation culture with a total temperatu...

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Abstract

The invention discloses a preparation method of solid active cordyceps sinensis mycelia isolated from culture media. Cordyceps sinensis strains with stable characters are obtained by screening and domesticating cordyceps sinensis strains. The solid active cordyceps sinensis mycelia dispensing with purification and deep processing are obtained by carrying out enlarge culture, fermentation culture and isolation culture on liquid step by step, adopting certain fermentation culture media formulas, isolation culture media formulas and the like and undergoing certain temperature difference and the processes such as light stimulation, isolation culture method and the like in the culture process. The content of the solid active cordyceps sinensis mycelia is far higher than the content of existing cordyceps sinensis mycelia prepared manually, thereby being more suitable for large-scale industrial production. The prepared cordyceps sinensis mycelia can be directly eaten without being processed, thus furthest retaining the bioactivity of the cordyceps sinensis mycelia; moreover, the prepared cordyceps sinensis mycelia have the advantages of faint scent, good taste, convenience and fastness in eating, high medicinal health function and the like.

Description

technical field [0001] The invention relates to the field of health food production, in particular to a method for preparing solid active Cordyceps mycelium separated from culture medium Background technique [0002] With the improvement of people's living standards and the increasing frequency of social activities, the burden of successful people from all walks of life and the majority of young and middle-aged workers has further increased. The middle-aged and elderly people and some children and children urgently need to improve and enhance their physical fitness. For this purpose, high-grade natural nutrition has been developed. Health food has become an important field waiting to be developed. [0003] Cordyceps sinensis is a nourishing and strong traditional Chinese medicine with a long history of medicinal use in my country. Modern pharmacological and pharmacological studies have shown that it contains adenosine, mannitol and 18 kinds of amino acids necessary for the h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 杨秀芬
Owner 广西华夏医药科技有限公司