Method used for increasing 2,3-butylene glycol yield

A technology for butanediol and yield, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield of 2,3-butanediol, achieve reduced wastewater treatment volume, simple operation, The effect of improving synthesis concentration and conversion rate

Active Publication Date: 2014-07-09
SINOPEC SHANGHAI ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is the lower problem of 2,3-butanediol production in the prior art, and a new method for increasing 2,3-butanediol production is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The strains stored in glycerol tubes were transferred to solid medium and activated at 37°C for 12h. Inoculate the 1-loop activated strain into a 250mL Erlenmeyer flask containing 50mL of liquid seed medium, and culture it for 12h in a table-top constant temperature (37°C) shaker with a rotation speed of 120rpm to obtain seed cells.

[0024] Transfer 1 mL of the activated bacteria to 50 mL of a liquid medium with a glucose concentration of 50 g / L, and after 24 hours of culture in a shake flask, transfer 1 mL of the bacteria to a 50 mL liquid medium with a glucose concentration of 100 g / L , cultivated for 24h. Go down in sequence, gradually increase the concentration of glucose in the medium, after continuous transfer culture of 150g / L, 200g / L, 250g / L, 300g / L, 350g / L, dilute the bacterial solution and spread it on a glucose concentration of 300g / L agar plate, isolate a single colony, and subculture on the same agar plate for 16 generations to stabilize its physiologica...

Embodiment 2

[0027] According to the method and steps described in Example 1, the antibiotic ampicillin was not added when the fermentation was 8 hours, and the antibiotic cephalosporin was added when the fermentation was 16 hours, so that the concentration of the cephalosporin in the fermentation broth was 75 mg / L. The average concentration of 2,3-butanediol was 68g / L.

Embodiment 3

[0029] According to the method and steps described in Example 1, only the antibiotic ampicillin was not added during fermentation for 8 hours, and the antibiotic polymyxin B was added, so that the concentration of polymyxin B in the fermentation broth was 100 mg / L. The average concentration of 2,3-butanediol was 68.4g / L.

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Abstract

The invention relates to a method used for increasing 2,3-butylene glycol yield. The method is mainly used for solving a problem of existing technology that yield of 2,3-butylene glycol is relatively low. According to the method, bacteria are subjected to high sugar resistance domestication firstly; the domesticated bacteria are used for fermentation culturing, and an antibiotic is added in fermentation culturing processes, wherein the antibiotic is at least one selected from penicillin, cephalosporin, bacillus, D-cycloserine, and the like which are capable of inhibiting cell wall synthesis, and polymyxin B, nystatin, amphotericin, or valinomycin which are capable of influencing cytomembrane functions, and adding concentration of the antibiotic ranges from 0.0001 to 5g / L. The method is capable of increasing 2,3-butylene glycol yield, and can be used for production of 2,3-butylene glycol.

Description

technical field [0001] The present invention relates to a method for increasing the production of 2,3-butanediol. Background technique [0002] 2,3-butanediol is a kind of important compound, can be widely used in multiple fields such as chemical industry, food, fuel and aerospace (Syu M J.Applied Microbiology and Biotechnology, 2001,55 (1): 10-18 ). Its dehydration product methyl ethyl ketone is a low-boiling solvent, which can be used in coatings, adhesives, lubricants, dyes, inks and other industries, and can also be used as an intermediate for organic synthesis of spices, antioxidants, etc.; at the same time, it can also be used as Used to produce rubber monomers such as 2-butene and 1,3-butadiene; the esterified form of 2,3-butanediol is the precursor for the synthesis of polyimine, which can be used in drugs, cosmetics, lotions, etc. ; The diacetylated form of 2,3-butanediol obtained by catalytic dehydrogenation can be used as a food additive with high-value spices. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/22C12R1/43C12R1/07C12R1/01
Inventor 杜军
Owner SINOPEC SHANGHAI ENG
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