12 results about "Cell wall synthesis" patented technology

The invention discloses an application of a corn ERF transcription factor ZmERF75 in regulation and control of corn stalk development and cell wall composition. Researches find that by reducing the expression of the ZmERF75 gene (such as a zmerf75 mutant), the plant height, the stem diameter, the stem bending force and the leaf tension of the corn can be obviously reduced, and vascular bundles are sparsely distributed. Meanwhile, the stem cellulose content of the zmerf75 mutant is reduced, the lignin content is increased, the monosaccharide content is changed, and the saccharification efficiency is remarkably improved. An EMSA and yeast single hybridization experiment proves that the ZmERF75 protein can be specifically combined with DNA in a CUI gene promoter region. The invention discloses a key regulation and control effect of ZmERF75 in corn stalk development and cell wall synthesis, and provides a new gene target and theoretical basis for improving the biomass utilization efficiency of corn stalks, optimizing cell wall composition and improving stalk characters through a molecular breeding means.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The photosensitive compound comprises a beta-lactam ring as shown in a formula I and a Ce6 photosensitizer, and does not contain a quenching group. When the compound is in contact with MRSA and other gram positive bacteria, the beta-lactam ring structure in the compound is combined with MRSA and other gram positive bacteria cell wall synthesis key enzyme-transpeptidase to provide targeting for the photosensitizer Ce6, so that the targeting effect on the bacteria is realized, the specificity is strong, a large amount of active oxygen is generated under illumination to kill the bacteria, and the compound can be used as a photosensitizer Ce6. Meanwhile, damage to normal tissues is avoided, photodynamic therapy on bacterial infection is facilitated, and the photodynamic therapy has high efficiency and low tissue toxicity.

The invention discloses a breathable deodorant textile fabric and a preparation method thereof.The textile fabric comprises two layers of modified base cloth, a reinforced deodorization layer and a protective deodorization layer, the reinforced deodorization layer is arranged between the two layers of modified base cloth, and the protective deodorization layer is arranged on the side, away from the reinforced deodorization layer, of the modified base cloth; the reinforced deodorization layer of the textile fabric contains nano titanium dioxide, and the nano titanium dioxide has a quite strong oxidation function during illumination, can solidify protein of bacteria and viruses, inactivate the bacteria and viruses, destroy cell membranes, enable cytoplasm to run off, kill the bacteria and prevent the bacteria from breeding odor; the modified base cloth contains a bamboo fiber porous structure which can absorb odor, the surface of the modified base cloth is positively charged, bacteria and viruses can be adsorbed, division is inhibited, the bacteria and the viruses lose reproductive capacity, imidazole zinc in the deodorization layer is enhanced, the cell membrane function of the bacteria can be interfered, synthesis of cell walls can be destroyed, metabolism and reproduction of the bacteria can be inhibited, and the deodorization effect is achieved. The generation of odor is further prevented.

Disclosed are: yeast which is capable of highly producing squalene, the yeast being genetically modified in such a manner that the expression or activity of at least one enzyme in the ergosterol biosynthesis pathway can be increased or decreased and being also genetically modified in such a manner that the expression of a protein involved in the synthesis of a cell wall can be increased or decreased; and a method for producing squalene, the method including a step for culturing the yeast in a culture medium.

This invention provides a novel peptide adjuvant CAL185, its composition, and its application. The peptide adjuvant CAL185 and its series derivatives have the following sequences: X0-X1-X2-X3-X4-X5-X6-X 7‑ X8-X9-X 10 -X 11 -X 12 The non-ribosomal peptide adjuvant of this invention can bind to penicillin-binding protein 2a, targeting its allosteric site, and assisting β-lactam antibiotics (such as penicillins, cephalosporins, and carbapenems) in binding to the active site of penicillin-binding protein 2a, thereby enhancing their original low affinity, blocking cell wall synthesis, reversing drug resistance, and exerting a potent bactericidal effect. The peptide adjuvant of this invention, through synergy with the aforementioned antibiotics, exhibits potent bactericidal activity against clinically common methicillin-resistant Staphylococcus aureus, and holds promise for further development into a novel anti-infective drug adjuvant.

This invention discloses a traditional Chinese medicine monomer for treating necrotizing enteritis in poultry and its application, belonging to the field of traditional Chinese veterinary medicine technology. This monomer is composed of resveratrol, with a dosage of 125-500 mg per kilogram of feed, preferably 250 mg. The resveratrol of this invention treats necrotizing enteritis in chickens through a synergistic effect of direct in vitro antibacterial activity and in vivo intestinal barrier protection: the MIC against Clostridium perfringens in vitro is 0.16 mg / mL, exerting its antibacterial effect through multiple targets including inhibition of cell wall synthesis, energy metabolism, and virulence regulation; in vivo, it significantly improves the growth performance of NE broilers, reduces intestinal lesion scores, repairs the intestinal barrier, regulates immune inflammatory responses, and enhances antioxidant capacity. This invention provides theoretical and experimental basis for the development of natural control agents against intestinal pathogens in livestock and poultry, possessing the characteristics of high efficiency, safety, and no residue, and providing a new approach to replace antibiotics in the treatment of necrotizing enteritis in chickens.

This invention relates to the field of air disinfection devices, specifically to a composite intelligent circulating air disinfection device and method. The device includes a housing, and within the housing are a gas control device, a liquid control device, a light control device, a photoexcitation device, and a nanoenzyme adsorption device. This invention integrates modules corresponding to four methods: electrochemical strong oxidizing bactericidal liquid disinfection, ultraviolet irradiation disinfection, nanoenzyme disinfection, and photoexcitation disinfection. This enables highly efficient killing of various pathogens in the air, including bacteria, fungi, and viruses. Furthermore, through ultraviolet-assisted electrochemical oxidation technology, it downregulates the expression of bacterial cell wall synthesis genes and efflux pump genes, inhibits energy metabolism, and induces key gene mutations, effectively degrading and inactivating antibiotic resistance genes, blocking the spread of drug resistance. This significantly improves the quality and efficiency of air disinfection while ensuring the coexistence of humans and the machine.

The invention discloses a CbuPLT3a gene derived from catalpa bungei and an application of the CbuPLT3a gene in inhibition of synthesis of secondary cell walls of poplar trees. The nucleotide sequence of the CbuPLT3a gene is as shown in SEQ ID NO: 2. According to the invention, through an agrobacterium tumefaciens mediated genetic transformation method, the CbuPLT3a gene is introduced into the poplar '84K' (Popullus alba * Popullus glabullosa), so that an overexpression transgenic plant is obtained. Phenotypic analysis results show that compared with a wild type, the CbuPLT3a overexpression plant has the advantages that the thickness of a secondary cell wall is obviously reduced, the width of xylem is narrowed, and meanwhile, the plant height and the internode length of the plant are obviously reduced. The invention proves that CbuPLT3a is a gene capable of inhibiting synthesis of poplar secondary walls, and a new gene resource and a new technical strategy are provided for molecular design and breeding of forest wood quality.

The present invention provides a method for producing lipids that improve the buoyancy of microalgae and thus the recovery efficiency, and a transformed microalgae with improved buoyancy. [Solution] A method for producing lipids, comprising culturing a transformed microalgae that has undergone at least one modification selected from the group consisting of (A) to (C) below, producing fatty acids or lipids having fatty acids as components, recovering the transformed bodies by flotation separation, and obtaining lipids from the recovered transformed bodies. (A) Modifications that promote the expression of at least one gene encoding a triacylglycerol synthesis pathway-related protein. (B) Modifications that promote the expression of at least one gene encoding a Calvin cycle-related protein. (C) Modifications that suppress the expression of at least one gene encoding a cell wall synthesis pathway-related protein.

The present application relates to plant epidermis specific cell wall remodeling promotes meristem development and plant yield. Specifically, the present application provides a reagent for promoting plant epidermis specific expression of CSLD5 or CSLD4 protein or gene in promoting cell wall synthesis, improving the tissue mechanical properties of shoot apical meristem, and improving the traits of plants.

The invention discloses a bacteriostatic hand sanitizer and a preparation method thereof, and belongs to the technical field of hand sanitizers. The bacteriostatic hand sanitizer is prepared from the following raw materials in parts by weight: 100 to 150 parts of hand sanitizer matrix, 0.5 to 8 parts of tea tree essential oil, 0.5 to 1.5 parts of green tea carbon dots and 0.01 to 0.1 part of nisin. According to a triple antibacterial scheme, bacterial cell membranes become unstable and permeability is increased due to attack of tea carbon dots, and the tea tree essential oil deeply destroys the cell membrane structure by utilizing initial damage caused by the carbon dots, so that structural collapse of bacteria is caused; the nisin can be highly specifically combined with a key precursor synthesized by a bacterial cell wall, so that the formation of a peptidoglycan network is prevented, and the nisin and the peptidoglycan damage a bacterial membrane at present and can enter bacteria more smoothly; even if bacteria survive in early attack, the bacteria can be cracked and die due to the fact that new cell walls cannot be synthesized; and the cleaning agent has good dirt-removing power, and is safe and non-irritant.