25 results about "Cell wall synthesis" patented technology

The invention relates to the field of chemical synthesis and biomedical materials, and discloses a multifunctional nano-drug and a preparation method and antibacterial application thereof, and the multifunctional nano-drug is mainly composed of a copper sulfide nano-carrier, a bacterial targeting photosensitizer and glucose oxidase (GOx). Wherein the copper sulfide nano-carrier (CuS NPs) has a hollow mesoporous structure and can effectively load drug molecules. The bacterium targeting photosensitizer, namely, D-cysteine functional modified pentafluorophenyl mycogreen (FBC-Cys), can be used for targeting bacteria by utilizing the characteristic that free D-type amino acid is integrated to the end of peptidoglycan on the surface of a cell wall in the cell wall synthesis process of the bacteria. Glucose oxidase (GOx) can efficiently consume glucose in the bacterial microenvironment, and the hunger treatment effect is achieved. The hollow mesoporous copper sulfide nanoparticles are prepared by adopting a one-pot hydrothermal method, synthesis raw materials are easy to obtain, and the method is simple and rapid. And the FBC-Cys and the GOx are loaded to the cavities and the surfaces of the copper sulfide nanoparticles through a physical adsorption effect. The multifunctional nano-drug can target bacterial cell walls, change the bacterial microenvironment, integrate photodynamic therapy and hunger therapy, and achieve a multifunctional antibacterial effect. The antibacterial hydrogel provided by the invention has excellent antibacterial effect and biocompatibility, and can play an excellent role in treatment of future bacterial infection.

The invention discloses a preparation method of antibacterial enhanced ABS (acrylonitrile-butadiene-styrene) plastic, and relates to the field of ABS plastic. A hyperbranched polylactic acid network and an ABS matrix form an interpenetrating structure, and molecular chain slippage is jointly inhibited by a cross-linking point of trimethylolpropane triacrylate and a physical enhancement effect of nano SiO2, so that the tensile strength and the impact strength of the material are improved; bacterial cell membranes are destroyed through a quaternary ammonium salt group with positive electricity, synthesis of fungal cell walls is interfered and formation of biological membranes is inhibited through boric acid molecules, hypha colonization is physically hindered through high crosslinking density of a hyperbranched PLA network, and therefore the bacteriostasis rate on bacteria and fungi is increased.

The invention discloses a fusarium venetianum engineering strain as well as a construction method and application thereof, relates to the technical field of gene engineering, and aims to solve the problem of low fungal hypha protein yield, chitin synthetase FvChs4 gene of fusarium venetianum is deleted, the classification name of the fusarium venetianum engineering strain is fusarium venetianum, and the fusarium venetianum engineering strain has the characteristics that the fusarium venetianum engineering strain has a nucleotide sequence shown in SEQ ID NO: 1 and a nucleotide sequence shown in SEQ ID NO: 2 and a nucleotide sequence shown in SEQ ID NO: 3. The fusarium venanii engineering strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 27, 2025, and the preservation number is CGMCC No. 42015. Through targeted knockout of the FvChs4 gene, consumption of a carbon source by cell wall synthesis is reduced, and directional enrichment of the carbon source to protein synthesis is realized, so that the yield of hypha protein is increased.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The invention discloses a high-yield cell-free protein synthesis system based on a cell crude extract and application of the high-yield cell-free protein synthesis system. The system comprises an escherichia coli extract, a DNA template, a buffer solution, an amino acid mixture, a nucleoside triphosphate mixture, an ATP regeneration system and antibiotics, the antibiotics are selected from compounds having inhibitory activity on metabolic processes except ribosome functions in prokaryotic microorganisms, including but not limited to one or more combinations of interfering DNA replication, DNA transcription, cell wall synthesis, energy metabolism, lipid synthesis or other non-translational related metabolic pathways. According to the method disclosed by the invention, the escherichia coli crude extract is used as a bacterial chassis, and non-essential metabolic pathways in a CFPS system are selectively inhibited by introducing different types of antibiotics, so that the yield of protein synthesis is increased.

The invention discloses an application of a corn ERF transcription factor ZmERF75 in regulation and control of corn stalk development and cell wall composition. Researches find that by reducing the expression of the ZmERF75 gene (such as a zmerf75 mutant), the plant height, the stem diameter, the stem bending force and the leaf tension of the corn can be obviously reduced, and vascular bundles are sparsely distributed. Meanwhile, the stem cellulose content of the zmerf75 mutant is reduced, the lignin content is increased, the monosaccharide content is changed, and the saccharification efficiency is remarkably improved. An EMSA and yeast single hybridization experiment proves that the ZmERF75 protein can be specifically combined with DNA in a CUI gene promoter region. The invention discloses a key regulation and control effect of ZmERF75 in corn stalk development and cell wall synthesis, and provides a new gene target and theoretical basis for improving the biomass utilization efficiency of corn stalks, optimizing cell wall composition and improving stalk characters through a molecular breeding means.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The photosensitive compound comprises a beta-lactam ring as shown in a formula I and a Ce6 photosensitizer, and does not contain a quenching group. When the compound is in contact with MRSA and other gram positive bacteria, the beta-lactam ring structure in the compound is combined with MRSA and other gram positive bacteria cell wall synthesis key enzyme-transpeptidase to provide targeting for the photosensitizer Ce6, so that the targeting effect on the bacteria is realized, the specificity is strong, a large amount of active oxygen is generated under illumination to kill the bacteria, and the compound can be used as a photosensitizer Ce6. Meanwhile, damage to normal tissues is avoided, photodynamic therapy on bacterial infection is facilitated, and the photodynamic therapy has high efficiency and low tissue toxicity.

The invention discloses a breathable deodorant textile fabric and a preparation method thereof.The textile fabric comprises two layers of modified base cloth, a reinforced deodorization layer and a protective deodorization layer, the reinforced deodorization layer is arranged between the two layers of modified base cloth, and the protective deodorization layer is arranged on the side, away from the reinforced deodorization layer, of the modified base cloth; the reinforced deodorization layer of the textile fabric contains nano titanium dioxide, and the nano titanium dioxide has a quite strong oxidation function during illumination, can solidify protein of bacteria and viruses, inactivate the bacteria and viruses, destroy cell membranes, enable cytoplasm to run off, kill the bacteria and prevent the bacteria from breeding odor; the modified base cloth contains a bamboo fiber porous structure which can absorb odor, the surface of the modified base cloth is positively charged, bacteria and viruses can be adsorbed, division is inhibited, the bacteria and the viruses lose reproductive capacity, imidazole zinc in the deodorization layer is enhanced, the cell membrane function of the bacteria can be interfered, synthesis of cell walls can be destroyed, metabolism and reproduction of the bacteria can be inhibited, and the deodorization effect is achieved. The generation of odor is further prevented.

The invention provides application and a method for inducing synthesis of a secondary cell wall of a rice leaf sheath thick-wall tissue and expression of a phenolic polymer in improving resistance of rice to brown planthopper, and belongs to the technical field of gene engineering. Chemical elicitors are utilized to excite rice in different growth periods to induce synthesis of cell walls in rice leaf sheaths and expression of phenolic polymers, so that resistance to brown planthoppers is generated; the chemical exciton is trifluorobenzene pyrimidine; the base sequences of the genome I participating in the synthesis of the rice leaf sheath cell wall are respectively as shown in SEQ ID NO: 7 to SEQ ID NO: 24; the phenolic polymer is formed by consuming flavonoid by peroxidase by taking hydrogen peroxide as an electron acceptor; the base sequences of the genome II participating in peroxidase expression are respectively shown as SEQ ID NO: 25 to SEQ ID NO: 42.

Disclosed are: yeast which is capable of highly producing squalene, the yeast being genetically modified in such a manner that the expression or activity of at least one enzyme in the ergosterol biosynthesis pathway can be increased or decreased and being also genetically modified in such a manner that the expression of a protein involved in the synthesis of a cell wall can be increased or decreased; and a method for producing squalene, the method including a step for culturing the yeast in a culture medium.

The present invention relates to the field of bioactive peptides, and specifically to an antifungal bovine liver peptide, a preparation method, a pharmaceutical composition and applications thereof. The antifungal bovine liver peptide has an amino acid sequence of RWKWQWKRWLKKLGAPSFRWCVRRA, and acts simultaneously on fungal membranes, cell walls and intracellular targets, synergistically exerting antifungal effects through three mechanisms: membrane destruction, cell wall synthesis interference and nucleic acid binding, thereby greatly reducing the risk of drug resistance.

This invention provides a novel peptide adjuvant CAL185, its composition, and its application. The peptide adjuvant CAL185 and its series derivatives have the following sequences: X0-X1-X2-X3-X4-X5-X6-X 7‑ X8-X9-X 10 -X 11 -X 12 The non-ribosomal peptide adjuvant of this invention can bind to penicillin-binding protein 2a, targeting its allosteric site, and assisting β-lactam antibiotics (such as penicillins, cephalosporins, and carbapenems) in binding to the active site of penicillin-binding protein 2a, thereby enhancing their original low affinity, blocking cell wall synthesis, reversing drug resistance, and exerting a potent bactericidal effect. The peptide adjuvant of this invention, through synergy with the aforementioned antibiotics, exhibits potent bactericidal activity against clinically common methicillin-resistant Staphylococcus aureus, and holds promise for further development into a novel anti-infective drug adjuvant.

This invention discloses a traditional Chinese medicine monomer for treating necrotizing enteritis in poultry and its application, belonging to the field of traditional Chinese veterinary medicine technology. This monomer is composed of resveratrol, with a dosage of 125-500 mg per kilogram of feed, preferably 250 mg. The resveratrol of this invention treats necrotizing enteritis in chickens through a synergistic effect of direct in vitro antibacterial activity and in vivo intestinal barrier protection: the MIC against Clostridium perfringens in vitro is 0.16 mg / mL, exerting its antibacterial effect through multiple targets including inhibition of cell wall synthesis, energy metabolism, and virulence regulation; in vivo, it significantly improves the growth performance of NE broilers, reduces intestinal lesion scores, repairs the intestinal barrier, regulates immune inflammatory responses, and enhances antioxidant capacity. This invention provides theoretical and experimental basis for the development of natural control agents against intestinal pathogens in livestock and poultry, possessing the characteristics of high efficiency, safety, and no residue, and providing a new approach to replace antibiotics in the treatment of necrotizing enteritis in chickens.

This invention relates to the field of air disinfection devices, specifically to a composite intelligent circulating air disinfection device and method. The device includes a housing, and within the housing are a gas control device, a liquid control device, a light control device, a photoexcitation device, and a nanoenzyme adsorption device. This invention integrates modules corresponding to four methods: electrochemical strong oxidizing bactericidal liquid disinfection, ultraviolet irradiation disinfection, nanoenzyme disinfection, and photoexcitation disinfection. This enables highly efficient killing of various pathogens in the air, including bacteria, fungi, and viruses. Furthermore, through ultraviolet-assisted electrochemical oxidation technology, it downregulates the expression of bacterial cell wall synthesis genes and efflux pump genes, inhibits energy metabolism, and induces key gene mutations, effectively degrading and inactivating antibiotic resistance genes, blocking the spread of drug resistance. This significantly improves the quality and efficiency of air disinfection while ensuring the coexistence of humans and the machine.

The invention discloses a CbuPLT3a gene derived from catalpa bungei and an application of the CbuPLT3a gene in inhibition of synthesis of secondary cell walls of poplar trees. The nucleotide sequence of the CbuPLT3a gene is as shown in SEQ ID NO: 2. According to the invention, through an agrobacterium tumefaciens mediated genetic transformation method, the CbuPLT3a gene is introduced into the poplar '84K' (Popullus alba * Popullus glabullosa), so that an overexpression transgenic plant is obtained. Phenotypic analysis results show that compared with a wild type, the CbuPLT3a overexpression plant has the advantages that the thickness of a secondary cell wall is obviously reduced, the width of xylem is narrowed, and meanwhile, the plant height and the internode length of the plant are obviously reduced. The invention proves that CbuPLT3a is a gene capable of inhibiting synthesis of poplar secondary walls, and a new gene resource and a new technical strategy are provided for molecular design and breeding of forest wood quality.

The invention discloses biological peptide antibacterial mouthwash as well as a preparation method and application thereof. The mouthwash comprises small molecule peptides shown as SEQ NO.1, SEQ NO.2 and SEQ NO.3 in a specific proportion, and auxiliaries such as glycerol, sodium hyaluronate and cocamidopropyl betaine. The small molecule peptides play roles in oral bacterial cell wall synthesis, cell membrane transport and cell membrane energy metabolism related proteins respectively, and a synergistic antibacterial effect is generated after combination. According to the present invention, the inhibition zone test and the minimum inhibitory concentration determination results show that the antibacterial effect of the mouthwash on streptococcus mutans, porphyromonas gingivalis and fusobacterium nucleatum is significantly superior to the traditional mouthwash and the single biological peptide mouthwash, and the mouthwash has advantages of good biocompatibility, low toxicity, effective oral cavity cleaning and oral cavity disease prevention, and is suitable for daily oral cavity nursing.

The invention relates to the field of plant protection, and particularly discloses a bactericidal composition containing components (A) and (B) and application of the bactericidal composition. A component (A) in the bactericidal composition is a compound with a formula (I), component (B) is selected from the group consisting of respiration inhibitors, sterol biosynthesis inhibitors, nucleic acid synthesis inhibitors, cell division and cytoskeleton inhibitors, amino acid and protein synthesis inhibitors, signal transduction inhibitors, lipid and membrane synthesis inhibitors, inhibitors having a multi-site action, and cell wall synthesis inhibitors. The composition disclosed by the invention can be used for controlling or preventing plants from being infected by plant pathogenic microorganisms in agriculture or horticulture. # imgabs0 #

The present invention provides a method for producing lipids that improve the buoyancy of microalgae and thus the recovery efficiency, and a transformed microalgae with improved buoyancy. [Solution] A method for producing lipids, comprising culturing a transformed microalgae that has undergone at least one modification selected from the group consisting of (A) to (C) below, producing fatty acids or lipids having fatty acids as components, recovering the transformed bodies by flotation separation, and obtaining lipids from the recovered transformed bodies. (A) Modifications that promote the expression of at least one gene encoding a triacylglycerol synthesis pathway-related protein. (B) Modifications that promote the expression of at least one gene encoding a Calvin cycle-related protein. (C) Modifications that suppress the expression of at least one gene encoding a cell wall synthesis pathway-related protein.

The present application relates to plant epidermis specific cell wall remodeling promotes meristem development and plant yield. Specifically, the present application provides a reagent for promoting plant epidermis specific expression of CSLD5 or CSLD4 protein or gene in promoting cell wall synthesis, improving the tissue mechanical properties of shoot apical meristem, and improving the traits of plants.

The invention relates to the technical field of biology, and discloses application of a GhEPC1 gene in regulation and control of cotton verticillium wilt resistance. How the GhEPC1 gene affects cell wall synthesis is analyzed by means of biochemistry, molecular biology, cell histology, genetics and the like so as to regulate and control a molecular mechanism of cotton resistance to verticillium wilt bacteria; the GhEPC1 gene disease-resistant mechanism can be revealed from the perspective of molecular level, and new theoretical basis and technical support are provided for cotton molecular breeding.

The invention relates to the field of bioactive peptides, in particular to an antifungal bovine liver peptide, a preparation method, a pharmaceutical composition and application of the antifungal bovine liver peptide, the amino acid sequence of the antifungal bovine liver peptide is RWKWQWKRWLKKLGAPSFRWCVRRA, the antifungal bovine liver peptide acts on fungal membranes, cell walls and intracellular targets at the same time, and the antifungal bovine liver peptide can be used for preparing antifungal drugs. Three mechanisms of membrane damage, cell wall synthesis interference and nucleic acid combination synergistically play an antifungal role, so that the risk of drug resistance generation is greatly reduced.

The invention belongs to the technical field of fermentation engineering, and relates to a preparation method of single-cell protein, in particular to a method for preparing single-cell protein based on methanol as a unique carbon source. According to the method for preparing the single-cell protein based on the methanol, a yarrowia lipolytica strain is used as a fermentation strain, a strain capable of adapting to high-concentration methanol is screened through adaptive laboratory induction optimization, key enzymes and genes in the cell wall synthesis process are recognized, and the protein content is increased by weakening cell wall synthesis; and the fermentation process is optimized, so that the yield and the quality of the protein can be effectively improved, and high-level production of the single-cell protein by taking methanol as a unique carbon source is successfully realized.

The invention relates to the field of pesticide preparations, and discloses a special-effect prevention and treatment agent for rice sheath blight disease and a preparation method thereof.The special-effect prevention and treatment agent for rice sheath blight disease is prepared from, by weight, 10-14 parts of thifluzamide, 15-19 parts of validamycin, 8-12 parts of bacillus subtilis powder, 3-5 parts of azadirachtin, 2-4 parts of chitinase and 1-3 parts of beta-1, 3, 4-triazole-1-one. 100 parts of the cleaning agent comprise 1-3 parts of 1, 3-glucanase, 3-5 parts of sodium lignin sulfonate, 2-3 parts of modified polyether organosilicone, 1.5-2 parts of sodium carboxymethyl cellulose, 2-4 parts of lauryl sodium sulfate, 3-5 parts of sodium alginate oligosaccharide, 1-2 parts of nanoscale zinc oxide and the balance of kaolin. Through fusion of thifluzamide and validamycin, starting from different action mechanisms of interfering germ energy metabolism, inhibiting cell wall synthesis and the like, precise striking is performed on rhizoctonia solani, the instant sterilization effect is improved, and meanwhile, through collocation with biological enzymes such as chitinase and beta-1, 3-glucanase, key components of germ cell walls are decomposed in a targeted manner.

The invention discloses a bacteriostatic hand sanitizer and a preparation method thereof, and belongs to the technical field of hand sanitizers. The bacteriostatic hand sanitizer is prepared from the following raw materials in parts by weight: 100 to 150 parts of hand sanitizer matrix, 0.5 to 8 parts of tea tree essential oil, 0.5 to 1.5 parts of green tea carbon dots and 0.01 to 0.1 part of nisin. According to a triple antibacterial scheme, bacterial cell membranes become unstable and permeability is increased due to attack of tea carbon dots, and the tea tree essential oil deeply destroys the cell membrane structure by utilizing initial damage caused by the carbon dots, so that structural collapse of bacteria is caused; the nisin can be highly specifically combined with a key precursor synthesized by a bacterial cell wall, so that the formation of a peptidoglycan network is prevented, and the nisin and the peptidoglycan damage a bacterial membrane at present and can enter bacteria more smoothly; even if bacteria survive in early attack, the bacteria can be cracked and die due to the fact that new cell walls cannot be synthesized; and the cleaning agent has good dirt-removing power, and is safe and non-irritant.