43 results about "Cell wall synthesis" patented technology

Methods are provided for increasing plant growth rate, biomass and tolerance to stress by genetically engineering plants to contain and express a gene of the ascorbic acid synthesis-cell wall synthesis network (e.g. GlcUA reductase, GLOase or MIOX). Transgenic plants that are genetically engineered in such a manner are also provided.

The invention discloses a construction method for double-gene mutation escherichia coli used for secretory expression of recombinant protein. According to the invention, pre-designed primers for the double-knockout-genes of cell membrane protein and cell wall synthetase gene are used for a PCR reaction so as to obtain gene targeting fragments of the double-knockout-gene, wherein two ends of each gene targeting fragment are 10 to 250 bp homologous sequences of a specific target gene and the middle part of each gene targeting fragment is a resistance labeled sequence; the fragments are respectively integrated into chromosomes of the host escherichia coli through electrotransformation so as to obtain a secretory double-gene mutation escherichia coli strain. The method provided by the invention increases the expression of recombinant exogenous proteins, reduces intracellular degradation of target proteins, decreases intracellular accumulation of the recombinant exogenous proteins and eradicates formation of inclusion bodies; the method discards a breaking process for cell walls, reduces pyrogen and simplifies separation and purification; thus, the purposes of reducing production cost and improving protein expression and product quality are achieved.

A method of rapidly evaluating the susceptibility of a strain of bacteria to a cell wall synthesis inhibiting antibiotic based on an assessment of cell enlargement in response to doses of the cell wall synthesis inhibiting antibiotic which are correlated to breakpoints of bacterial susceptibility.

A method of antibiotic susceptibility testing is disclosed, and includes the following steps: (A) providing a sample to be tested wherein the sample contains a microbe; (B) adding an antibiotic into the sample, wherein the antibiotic serves to inhibit cell wall synthesis; (C) checking the sample by dielectrophoresis and observing a shape change of the microbe; and (D) determining whether the microbe is susceptible to the antibiotic according to the shape change thereof. The present invention also discloses a method for determining a minimum inhibitory concentration of the antibiotic.