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Bacterial cellulose producing bacteria and method for using the bacterial strain to ferment bacterial cellulose

A bacterial cellulose and bacteria-producing technology, applied in the field of fermentation of bacterial cellulose and bacterial cellulose-producing bacteria, can solve the problems of increased production efficiency, long time, long production cycle, etc., to improve strength, improve skin, and enhance consistency Effect

Inactive Publication Date: 2016-08-17
GUANGDONG IND TECHN COLLEGE +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the shallow static fermentation process has disadvantages such as large floor area, long fermentation cycle, high labor intensity, and difficult control of fermentation conditions.
Chinese patent CN200710030072.0 discloses a method for producing high-fiber coconut fruit by deep layer and shallow layer static coupling fermentation, which can overcome some of the above-mentioned shortcomings, but there are still deficiencies, which are mainly reflected in that the time of the deep layer static fermentation stage is longer, resulting in the whole The production cycle is still long, and there is still a lot of room for improvement in production efficiency

Method used

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  • Bacterial cellulose producing bacteria and method for using the bacterial strain to ferment bacterial cellulose
  • Bacterial cellulose producing bacteria and method for using the bacterial strain to ferment bacterial cellulose
  • Bacterial cellulose producing bacteria and method for using the bacterial strain to ferment bacterial cellulose

Examples

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Embodiment 1

[0050] Example 1 Screening, domestication and isolation of bacteria cellulose producing bacterium Gluconacetobacter xylinus (Gluconacetobacter xylinus) BC13

[0051] The bacterial cellulose producing bacteria Gluconacetobacter xylinus (Gluconacetobacter xylinus) BC13 described in the present invention is isolated and screened from coconut water (sampled in Hainan Province) which is naturally fermented and produced film. The specific screening, domestication, isolation and product identification methods are as follows:

[0052] (1) Plate separation

[0053] Solid medium: glucose 20g / L, yeast extract 10g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.2g / L, agar 20g / L, fresh coconut water 50% (v / v), adjust pH5.5, 121 ℃ of sterilization 20min. Prepare a plate according to the solid medium formula and set aside. Under aseptic conditions, remove the bacterial film on the surface from the naturally fermented coconut water, cut 2 g into a sterile test tube, add 10 mL of sterile water, place...

Embodiment 2

[0071] The bacterial cellulose strain is Gluconacetobacter xylinus (Gluconacetobacter xylinus) BC13, which was preserved in China Center for Type Culture Collection (CCTCC) on April 10, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2014124;

[0072] (1) Expanded cultivation of seeds

[0073] Seed expansion medium preparation: glucose 2g, yeast extract 0.8g, MgSO 4 ·7H 2 0.02g, 50mL of coconut water (fresh coconut, broken shell to get water), 50mL of purified water, adjust pH5.5, put into a 500mL triangular bottle, wrap the mouth of the bottle with 8 layers of gauze, sterilize at 121°C for 20min, cool to 30°C, Insert 1 ring of slant strains (inoculated with a conventional inoculation loop), place in a shaking incubator at a constant temperature of 30°C and shake at 150r / min for 16 hours to obtain a seed solution;

[0074] (2) deep dynamic fermentation

[0075] Fermentation medium preparation: glucose 75g, yeast extract 12g, NH 4 C...

Embodiment 3

[0086] The bacterial cellulose strain is Gluconacetobacter xylinus (Gluconacetobacter xylinus) BC13, which was preserved in China Center for Type Culture Collection (CCTCC) on April 10, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2014124;

[0087] (1) Expanded cultivation of seeds

[0088] Seed expansion medium preparation: glucose 4g, yeast extract 1.6g, MgSO 4 ·7H 2 0.04g, 100mL of coconut water (fresh coconut, broken shell to get water), 100mL of purified water, adjust pH5.5, put into a 500mL triangular bottle, wrap the mouth of the bottle with 8 layers of gauze, sterilize at 121°C for 20min, cool to 30°C, Insert 1 ring of slant strains (inoculated with a conventional inoculation loop), place in a shaking incubator at a constant temperature of 30°C and shake at 150r / min for 16 hours to obtain a seed solution;

[0089] (2) deep dynamic fermentation

[0090] Fermentation medium preparation: glucose 100g, yeast extract 14.4g, NH...

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Abstract

The invention discloses a bacteria cellulose producing strain and a method for fermenting the bacteria cellulose by utilizing the same. The strain is gluconacetobacter xylinus BC13 and preserved in China Center for Type Culture Collection (CCTCC) on April 10, 2014, the address of CCTCC is Wuhan Universality of China, and the preservation number is CCTCC No. M2014124. The method for fermenting the bacteria cellulose comprises the following steps of fermenting by alternating the deep layer dynamic way and the shallow layer static way, wherein since the deep layer dynamic fermentation time is relatively short, no flocculent or spherical bacteria cellulose is formed; and completing the fermentation by adopting the shallow layer static way, wherein the final product is the membrane-shaped bacteria cellulose. Compared with the prior art, the method has the advantages that the production period can be obviously shortened, and the yield of the bacteria cellulose can be greatly increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacterial cellulose producing bacterium and a method for fermenting bacterial cellulose using the bacterial strain. Background technique [0002] Bacterial cellulose is extracellular cellulose produced by fermentation of microorganisms (mainly bacteria) in liquid media. In order to distinguish it from plant-derived cellulose, it is called bacterial cellulose or microbial cellulose. Compared with plant cellulose, it has the advantages of high crystallinity, high chemical purity, high tensile strength, high elastic modulus, strong water absorption and good biocompatibility. These advantages make it have great application potential in food, biomedicine, tissue engineering scaffold materials, acoustic equipment, cosmetics, membrane filtration materials, papermaking and other fields, and become a research hotspot of biomaterials at home and abroad. However, the current appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 邓毛程王瑶李静王文文孔爱琼何秀婷吴永辉谢拥葵
Owner GUANGDONG IND TECHN COLLEGE