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A kind of microbial microencapsulation product and preparation method thereof

A technology of microorganism and microencapsulation, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as unfavorable sphericity, and achieve the effect of ensuring survival rate

Active Publication Date: 2016-05-11
靖江市城中村投资建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of microcapsules of probiotics for feeding belongs to post-fermentation coating in technology, which requires high concentration of microbial cells to be coated in the microcapsule preparation materials, so it is not conducive to obtaining microcapsule products with good sphericity and uniform particles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation method of lactic acid bacteria microcapsule

[0021] The lactic acid bacteria seed solution was cultured at 37°C and 110r / min for 6 hours at an inoculation amount of 1% to obtain a lactic acid bacteria seed solution culture, so that the microbial density reached 2*10 6 cfu / ml; Centrifuge the obtained lactic acid bacteria seed liquid culture, collect the bacterial cell precipitate, add nano-structured calcium phosphate hollow microspheres accounting for 2% of the total weight of the bacterial cell precipitate to the bacterial cell precipitate, and the size of the nano-structured calcium phosphate hollow microsphere Mix with a high-speed homogenizer to obtain a core material; add 3% sodium alginate and 1% gelatin to distilled water and mix, and sterilize under high pressure to obtain an embedding agent. After the embedding agent is cooled to room temperature Add the core material and mix well, the volume ratio of the core material to the embe...

Embodiment 2

[0022] The preparation method of embodiment 2 butyric acid bacteria microcapsules

[0023] Cultivate the butyric acid bacteria seed liquid at 3% inoculation amount at 37°C and 110 r / min for 7 hours to obtain the butyric acid bacterial seed liquid culture, so that the microbial density reaches 2.5*10 6 cfu / ml; Centrifuge the obtained butyric acid bacteria seed liquid culture, collect the bacterial cell precipitate, add nano-structured calcium phosphate hollow microspheres accounting for 3% of the total weight of the bacterial cell precipitate to the bacterial cell precipitate, nano-structured calcium phosphate hollow microspheres The size is 4μm, the wall thickness is 50nm, and the high-speed homogenizer is mixed to obtain the core material; 3% sodium alginate and 1% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled to room temperature Then add the core material and mix well, the volume ratio of...

Embodiment 3

[0024] The preparation method of embodiment 3 Enterococcus faecium microcapsules

[0025] The seed liquid of Enterococcus faecium was cultivated at 37°C and 110r / min for 8 hours at an inoculum amount of 2% to obtain a culture of the seed liquid of Enterococcus faecium, so that the microbial density reached 3*10 6 cfu / ml; Centrifuge the obtained Enterococcus faecium seed liquid culture, collect the bacterial cell precipitate, add nano-structured calcium phosphate hollow microspheres accounting for 4% of the total weight of the bacterial cell precipitate to the bacterial cell precipitate, and nano-structured calcium phosphate hollow microspheres The ball size is 4μm, the wall thickness is 400nm, and the high-speed homogenizer is mixed to obtain the core material; 3% sodium alginate and 1% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled to After room temperature, add the core material and mix we...

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Abstract

The invention discloses a microbial capsule preparation method which comprises the step of using nano-structured calcium phosphate hollow microspheres to coat before fermentation. The invention further discloses a microbial microencapsulated product prepared by the method. According to the microbial microencapsulated product, coating is carried out before fermentation, so that the usage amount of thalli during microencapsulation is greatly reduced, the problems of low microencapsulation efficiency and low microencapsulation yield caused by influence resulting from thallus density during fermented embedding are avoided, and more stable microencapsulated products can be obtained; meanwhile, the nano-structured calcium phosphate hollow microspheres adopted by the preparation method of the microbial microencapsulated product have relatively large internal space, large specific surface area and low density, microbes can successfully enter the nano-structured calcium phosphate hollow microspheres from pore passages and are coated, so as to form a wrapping effect, and thus, the survival rate of the microbes is much higher than that of common non-microencapsulated treated microbes.

Description

technical field [0001] The invention belongs to the technical field of microbial microencapsulation, and in particular relates to a microbial microencapsulation product and a preparation method thereof. Background technique [0002] Lactic acid bacteria, butyric acid bacteria and Enterococcus faecium are normal probiotics in human and animal intestines. These probiotics are very resistant to high temperature in feed processing, especially in pelleting process, storage and transportation, and gastric acid and bile in gastrointestinal fluid. Poor, there are not many types and quantities of lactic acid bacteria that can enter the intestinal tract alive. [0003] Microencapsulation technology is the use of natural or polymer encapsulation materials to encapsulate solid, liquid or gas core substances to form microcapsules with semi-permeable or sealed capsules. At present, the preparation of microcapsules of probiotics for feeding is technically post-fermentation coating, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C12R1/01
Inventor 张志成张言召胡志华
Owner 靖江市城中村投资建设有限公司
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