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A tissue engineering scaffold suitable for the construction of human organs and its preparation method

A technology for tissue engineering scaffolds and organs, applied in prosthetics, medical science, etc., can solve the problems of material pore size, poor smoothness, poor biological and histocompatibility, and mismatched cell growth conditions, and achieve superior application value , Uniform pore size and low raw material cost

Inactive Publication Date: 2016-01-20
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural materials have poor formability and poor mechanical properties after molding, and their biodegradation rate is too fast, which does not match the growth conditions of cells. Some materials may also have immunogenicity, etc. Synthetic materials have good mechanical properties, but there are also It is difficult to degrade, does not match the growth rate of cells, the material has poor pore size and smoothness, and poor biological and tissue compatibility. Some natural-synthetic materials have overcome some problems, but problems in molding and degradation also plague the organization. engineering researchers

Method used

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  • A tissue engineering scaffold suitable for the construction of human organs and its preparation method
  • A tissue engineering scaffold suitable for the construction of human organs and its preparation method
  • A tissue engineering scaffold suitable for the construction of human organs and its preparation method

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Embodiment 1

[0037] A preparation method for a tissue engineering scaffold suitable for human organ construction, specifically comprising the following steps:

[0038] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0039] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0040] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0041] (2) Below 20°C, weigh 0.68ml of the sericin solution obtained in step (1) and 5ml of the chitosan solution, stir and mix evenly, then add 1ml of the sodium glycerophosphate solution obtained in step (1) dropwise, and stir 30min;

[0042] Put the mixed solution into the mold, control the temperature of the mold at 37°C, and make the mixed solution gel into shape; then place the molded material at -2...

Embodiment 2

[0044] A preparation method for a tissue engineering scaffold suitable for human organ construction, specifically comprising the following steps:

[0045] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0046] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0047] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0048] (2) Below 20°C, weigh 0.68ml of the sericin solution obtained in step (1) and 6ml of the chitosan solution, stir and mix evenly, then add 1ml of the sodium glycerophosphate solution obtained in step (1) dropwise, and stir 30min;

[0049] Put the mixed solution into the mold, control the temperature of the mold at 37°C, and make the mixed solution gel into shape; then place the molded material at ...

Embodiment 3

[0051] A preparation method for a tissue engineering scaffold suitable for human organ construction, specifically comprising the following steps:

[0052] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0053] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0054] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0055] (2) Below 20°C, weigh 0.68ml of the sericin solution obtained in step (1) and 7ml of the chitosan solution, stir and mix evenly, then add 1ml of the sodium glycerophosphate solution obtained in step (1) dropwise, and stir 30min;

[0056] Put the mixed solution into the mold, control the temperature of the mold at 37°C, and gel the mixed solution into shape; then place the molded material at -20°C...

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Abstract

The invention discloses a tissue engineering scaffold suitable for the construction of human organs. The pore diameter is 5-200 μm, the pore penetration is good, the degradability is appropriate, and the scaffold is suitable for the growth of cells therein. The invention also discloses a preparation method of the tissue engineering scaffold suitable for human organ construction. The preparation method is to prepare a mixed solution of sericin solution, chitosan solution and sodium glycerophosphate solution, and then mix the The solution is converted into a gel state, and then freeze-dried, placed back to soften, and treated with high-pressure steam to obtain a tissue engineering scaffold. By adjusting the ratio between the raw material sericin solution, chitosan solution and sodium glycerophosphate solution, or changing The process conditions can control the pore size and degradation performance of the obtained tissue engineering scaffold, so that it can meet the requirements for tissue engineering scaffolds for human organ construction; the preparation method has the advantages of mild reaction conditions, simple process, and low raw material cost, and is suitable for Industrialized scale-up production.

Description

technical field [0001] The invention belongs to the technical field of biological materials and tissue engineering, and in particular relates to a tissue engineering scaffold suitable for constructing human organs and a preparation method thereof. Background technique [0002] Tissue engineering is one of the research hotspots in the medical field today, and its birth brings hope for the regeneration of various functional tissues or organs. Surgical treatment methods reported in the literature often repair tissue defects by sacrificing normal tissue at the expense of surgical trauma. Not only the effect is not good, but also there are many complications. With the improvement of living standards, people pay more and more attention to their own health and safety, so more and more attention is paid to the research on tissue engineering scaffolds. All over the world, developing an ideal biological scaffold is an urgent problem in the field of tissue engineering and has become a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/58A61L27/56
Inventor 陈忠敏刘琼庞亚妮王富平李江峰
Owner CHONGQING UNIV OF TECH
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