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A stable homocysteine ​​detection kit

A homocysteine ​​and detection kit technology, which is applied to the determination/inspection of microorganisms, measuring devices, instruments, etc., can solve the problems of long-term storage of unfavorable reagents, cumbersome operation, and unfavorable promotion of detection reagents, and achieves life extension, The effect of improving antioxidant properties and prolonging the activity of enzymes

Active Publication Date: 2016-02-03
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to improve the stability of the homocysteine ​​detection reagents currently on the market, the detection reagents are mostly set to four reagents or three reagents, or even more reagents. Sometimes, it is necessary to manually mix the reagents in proportion on site and use them up as soon as possible, which not only increases the complexity of the operation, but also is not conducive to the long-term storage of the reagents, which is very unfavorable for the promotion of detection reagents on automatic biochemical instruments

Method used

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  • A stable homocysteine ​​detection kit
  • A stable homocysteine ​​detection kit
  • A stable homocysteine ​​detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (comparative example)

[0034] This example describes the formulation of a commercially available homocysteine ​​detection reagent, and the present invention will be further described in conjunction with the examples below.

[0035] 1. Reagent composition:

[0036] Reagent 1 (R1):

[0037] EDTA.2Na (disodium ethylenediaminetetraacetic acid) 0.5mM

[0038] ATP80mM

[0039] S-adenosylmethionine (SAM) 0.1mM

[0040] NADH0.3mM

[0041] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 0.5mM

[0042] α-ketoglutarate 5.0mM

[0043] Sodium azide 0.1g / L

[0044] The solvent is phosphate buffered saline pH7.1, 37°C;

[0045] Reagent 2 (R2):

[0046] Reagent 2a

[0047] Phosphate buffer pH7.8, 37°C

[0048] Hcy methyltransferase (HMTase) 5.0KU / L

[0049] Glutamate dehydrogenase (GLDH) 10KU / L

[0050] Reagent 2b

[0051] Phosphate buffer pH7.8, 37°C

[0052] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 2.5KU / L

[0053] Adenosine deaminase...

Embodiment 2

[0066] one, Reagent components

[0067] Reagent 1 (R1)

[0068] TRIS (trishydroxymethylaminomethane) buffer, Ph7.1

[0069] EDTA.2Na (disodium ethylenediaminetetraacetic acid) 0.1mM

[0070] ATP0.1mM

[0071] S-adenosylmethionine (SAM) 0.1mM

[0072] NADH0.3mM

[0073] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 0.5mM

[0074] α-ketoglutarate 5mM

[0075] Bovine serum albumin 0.1g / L

[0076] Sodium azide 0.1g / L;

[0077] Reagent 2 (R2)

[0078] TRIS (trishydroxymethylaminomethane) solution, Ph7.8

[0079] Hcy methyltransferase (HMTase) 0.5KU / L

[0080] Glutamate dehydrogenase (GLDH) 1KU / L

[0081] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 2.5KU / L

[0082] Adenosine deaminase (ADA) 1KU / L

[0083] Bovine serum albumin 0.1g / L

[0084] Polyethylene glycol 1g / L

[0085] Potassium ferrocyanide 15umol / L.

[0086] 2. Operation method:

[0087] This method is a validated stable homocysteine ​​detection reagent. The detection operation method is the s...

Embodiment 3

[0095] This example provides a formulation of a stable homocysteine ​​detection reagent after the amount of reagent materials is set to a high limit

[0096] Reagent 1 (R1):

[0097] TRIS (trishydroxymethylaminomethane) buffer, Ph7.1

[0098] EDTA.2Na (disodium ethylenediaminetetraacetic acid) 20mM

[0099] ATP90mM

[0100] S-adenosylmethionine (SAM) 1mM

[0101] NADH1mM

[0102] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 1mM

[0103] α-ketoglutarate 10mM

[0104] Bovine serum albumin 1g / L

[0105] Sodium azide 1g / L;

[0106] Reagent 2 (R2)

[0107] TRIS (trishydroxymethylaminomethane) solution, Ph7.8

[0108] Hcy methyltransferase (HMTase) 5.0KU / L

[0109] Glutamate dehydrogenase (GLDH) 5.0KU / L

[0110] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 5.0KU / L

[0111] Adenosine deaminase (ADA) 5.0KU / L

[0112] Bovine serum albumin 1g / L

[0113] Polyethylene glycol 5g / L

[0114] Potassium ferrocyanide 25umol / L 。

[0115] operation method

[0116]...

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Abstract

The present invention provides a stable homocysteine ​​detection kit, which consists of reagent 1 and reagent 2. The kit provided by the present invention improves the previous three reagents or even more reagents. The trouble of manual mixing in proportion has played a very good role in the use and promotion of the reagent in automatic biochemical analysis instruments; at the same time, the components of the reagent have been improved, so that the reagent has better stability.

Description

technical field [0001] The invention relates to a stable homocysteine ​​detection kit, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Homocysteine ​​(Hcy), also known as homocysteine, is an important intermediate product of the catabolism of sulfur-containing amino acids, which is homologous to cysteine, and does not participate in body protein synthesis. Plasma Hcy is a general term, referring to all forms of Hcy in plasma, including reduced Hey, disulfide Hcy, mixed disulfide cysteine-Hcy and mixed disulfide protein-Hey: under normal circumstances, there is little free Hey, mainly in the form of protein form. Many factors such as genetics, nutrition, renal insufficiency, drugs, hormones and other factors may affect the metabolism of homocysteine ​​in the body, resulting in hyperheyemia. A large number of studies have proved that high Hey is a new and important independent risk factor for heart, brain, and peripheral vas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/48
CPCG01N33/535G01N33/6815
Inventor 谭柏清罗维晓甘宜梧王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD