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A preparation method of slow-release drug microcapsules loaded with recombinant human growth hormone

A technology of human growth hormone and slow-release drugs, applied in the field of medicine, can solve the problems of low bioavailability, complex preparation process, weak stability, etc., and achieve the effect of high biological activity, simple preparation process and good stability

Active Publication Date: 2016-05-11
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the preparation of sustained-release drug microcapsules with single-armed polyethylene glycol-polyester linear polymers as carrier materials contains emulsifiers such as polyvinyl alcohol as auxiliary materials, and the preparation method needs to first form double emulsions, making the preparation process difficult. Complex, weak stability, poor reproducibility, low bioavailability

Method used

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  • A preparation method of slow-release drug microcapsules loaded with recombinant human growth hormone
  • A preparation method of slow-release drug microcapsules loaded with recombinant human growth hormone
  • A preparation method of slow-release drug microcapsules loaded with recombinant human growth hormone

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Embodiment 1

[0028] Example 1 A preparation method of microcapsules loaded with recombinant human growth hormone sustained release drug is as follows:

[0029] (1) The slow-release drug microcapsules carrying recombinant human growth hormone use a multi-armed polyethylene glycol-polyester block polymer as a carrier to carry recombinant human growth hormone; The polyester block polymer is four-armed polyethylene glycol 2000 - Polylactic acid 4000 Block polymer; four-armed polyethylene glycol 2000 - Polylactic acid 4000 The weight percentage of block polymer carrier and recombinant human growth hormone is 54%: 46%;

[0030] (2) The steps and conditions of the slow-release drug microcapsule preparation method are as follows:

[0031] ① Preparation of polymer hollow microcapsules: take four-armed polyethylene glycol 2000 - Polylactic acid 4000 Put 10 mg of block polymer in the reactor, add 20 mL of chloroform, the ratio of the mass mg of the multi-arm polyethylene glycol-polyester block ...

Embodiment 2

[0036] Embodiment 2: A preparation method of microcapsules loaded with recombinant human growth hormone sustained release drug is as follows:

[0037] (1) The slow-release drug microcapsules carrying recombinant human growth hormone use a multi-armed polyethylene glycol-polyester block polymer as a carrier to carry recombinant human growth hormone; The polyester block polymer is four-armed polyethylene glycol 2000 - Polylactic acid 7000 Block polymer; four-armed polyethylene glycol 2000 - Polylactic acid 7000 The weight percentage of block polymer carrier and recombinant human growth hormone is 43%:57%.

[0038] (2) The steps and conditions of the slow-release drug microcapsule preparation method are as follows:

[0039] ① Preparation of polymer hollow microcapsules: take four-armed polyethylene glycol 2000 - Polylactic acid 7000 Block polymer 10mg is placed in the reactor, add 5mL chloroform, four-armed polyethylene glycol 2000 - Polylactic acid 7000 The ratio of the ...

Embodiment 3

[0044] Embodiment 3: A preparation method of microcapsules loaded with recombinant human growth hormone sustained release drug is as follows:

[0045] (1) The slow-release drug microcapsules carrying recombinant human growth hormone use a multi-armed polyethylene glycol-polyester block polymer as a carrier to carry recombinant human growth hormone; The polyester block polymer is four-armed polyethylene glycol 2000 - Polylactic acid 10000 Block polymer; four-armed polyethylene glycol 2000 - Polylactic acid 10000 The weight percentage of the block polymer carrier and the recombinant human growth hormone is 30%:70%.

[0046] (2) The steps and conditions of the slow-release drug microcapsule preparation method are as follows:

[0047] ① Preparation of polymer hollow microcapsules: take four-armed polyethylene glycol 2000 - Polylactic acid 10000 Block polymer 10mg is placed in the reactor, add 10mL chloroform, four-armed polyethylene glycol 2000 - Polylactic acid 10000 The ...

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Abstract

The invention relates to a method for preparing recombinant human growth hormone entrapped sustained-release drug microcapsules. According to the method, a degradable multi-arm polyethylene glycol-polylactic acid block polymer material with good biocompatibility is taken as a vector material, and raw materials are easily obtained; the multi-arm polymer has relatively small hydrodynamic volume and relatively low viscosity, and the degradation rate of the multi-arm polymer is higher than that of linear polymers, so that the loading and delivery of drugs are more facilitated; a multi-arm polyethylene glycol-polylactic acid block copolymer has good drug loading capacity and unique amphiphilicity, so that protein adsorption can be prevented, the recognition and phagocytosis of a reticuloendothelial system are avoided, and the physiological barrier is easy to pass; the method disclosed by the invention is simple and does not need a complicated re-emulsion formation process; the microcapsules are good in stability, the bioavailability of recombinant human growth hormone is improved, and the content of the recombinant human growth hormone reaches up to 46-81%; the entrapment rate of the recombinant human growth hormone entrapped sustained-release drug microcapsules reaches 91%, the stability is good, the grain size is uniform, and the repeatability is good, so that the recombinant human growth hormone entrapped sustained-release drug microcapsules are suitable for large-scale production.

Description

technical field [0001] The invention relates to a preparation method of microcapsules carrying recombinant human growth hormone slow-release medicine, which belongs to the technical field of medicine. technical background [0002] In 1920, people discovered the existence of growth hormone (human growth hormone, hGH). Growth hormone is a protein secreted by pituitary cells. It is a peptide hormone consisting of 191 amino acid residues and a relative molecular mass of 22124 Daltons. There are four α-helices in the molecular structure of growth hormone, so that growth hormone can be well combined with its receptor. In 1958, it was discovered that human growth hormone could be used to treat dwarfism. However, at that time, human growth hormone could only be extracted from the pituitary gland of the corpse, and the quantity was limited, so it was difficult to guarantee the clinical dosage, which limited its application. In 1986, Boyer successfully synthesized recombinant human g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K38/27A61K47/34A61P3/00
Inventor 李云辉李文亮焦虎平杨秀云李磊姣马玉芹高莹
Owner CHANGCHUN UNIV OF SCI & TECH
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