Preparation and application of a csxwo3-like peroxide mimetic enzyme
A technology of peroxidation and simulating enzyme, applied in the field of nanotechnology and enzyme catalysis, can solve the problems of limited natural enzyme source, high price, application limitation, etc., and achieve the effects of short reaction time, low cost and low cost
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Embodiment 1
[0033] At a temperature of 35°C, 50 μg / ml of Cs x WO 3 Nanomaterials were added to 2.275ml of PBS (pH=4.0) buffer solution dissolved with TMB (0.6mM), and 50mM H 2 o 2 solution, reacted for 30min, and then measured the absorbance at 652nm on a UV spectrophotometer (see figure 1 ); figure 1 The results show that Cs x WO 3 group has obvious absorption at 652nm, indicating that Cs x WO 3 Nanomaterials have peroxidase-like activity and can catalyze H 2 o 2 Oxidation of TMB produces a color reaction.
Embodiment 2
[0035] At room temperature, 50 μg / ml of Cs x WO 3 Nanomaterials were added to PBS buffer solution (pH=4.0) containing 1mM terephthalic acid, and 50mM H 2 o 2 solution, start timing, at this time Cs x WO 3 Nanomaterials can catalyze H 2 o 2 Hydroxyl radicals are produced, and the produced hydroxyl radicals react with the substrate terephthalic acid to produce fluorescent substances, whose maximum absorption wavelength is 425nm, such as figure 2 shown; figure 2 The results showed that as the reaction time increased, the fluorescence value became higher and higher, indicating that a large number of hydroxyl radicals were generated, which proved that Cs x WO 3 The catalytic mechanism of nanomaterials is to catalyze H 2 o 2 produce hydroxyl radicals.
Embodiment 3
[0037] Will Cs x WO 3 The nanomaterial aqueous solution was ultrasonically treated for 0.5h, then dropped on the copper grid, dried and then scanned by TEM (see image 3 ); image 3 Show the resulting Cs x WO 3 It is in the shape of nanorods, and the size is relatively uniform. The length of the nanorods is about 150nm and the width is 15nm.
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