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Preparation method of flax protein powder

A protein powder and flax technology, applied in the protein composition of vegetable seeds, plant protein processing, protein food ingredients, etc., can solve the problems of limited economic value and failure to attract enough attention, and prevent heart and cerebrovascular diseases , reduce serum cholesterol, increase the effect of nutrition

Inactive Publication Date: 2014-08-20
YANTAI NEW ERA HEALTH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] my country is rich in flax resources, but flaxseed is mainly used for oil extraction, and its economic value is limited, so it has not attracted enough attention

Method used

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  • Preparation method of flax protein powder
  • Preparation method of flax protein powder
  • Preparation method of flax protein powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The influence of extracting liquid pH value on isolate protein extraction rate and protein content

[0021] 1. Test method

[0022] In the test, 5 treatment groups were established according to the pH value of the sodium hydroxide solution, and the pH value was respectively 7.5, 8.5, 9.5, 10.5, and 11.5. The specific steps of each group of experiments were as follows:

[0023] The defatted flax cake is crushed through a 100-180 mesh sieve, 100g of the sieve is taken, dissolved in 1000g of water, 22g of animal protein hydrolysis compound enzyme is added, shaken in a water bath at 40-60°C for 120min, filtered, and hydrogen is added to the filtrate Sodium oxide solution, the pH values ​​are 7.5, 8.5, 9.5, 10.5, 11.5 respectively, the mass ratio of the filtrate to the sodium hydroxide solution is 1:30, leaching at 60°C for 60 minutes, centrifuging, taking the supernatant and adding hydrochloric acid aqueous solution , control the pH to 5, precipitate out, cen...

Embodiment 2

[0030] Embodiment 2: The influence of extraction temperature on protein isolate extraction rate and protein content

[0031] 1. Test method

[0032] In the experiment, 5 treatment groups were set up according to the different extraction temperatures. The extraction temperatures were 40°C, 50°C, 60°C, 70°C, and 80°C. The specific steps of each group of experiments are as follows:

[0033] The defatted flax cake is crushed through a 100-180 mesh sieve, 100g of the sieve is taken, dissolved in 1000g, added with 22g of animal proteolytic compound enzyme, shaken in a water bath at 40-60°C for 120min, filtered, and added to the filtrate Sodium hydroxide solution with a value of 9.5, the mass ratio of filtrate to sodium hydroxide solution is 1:30, the extraction temperature is 40°C, 50°C, 60°C, 70°C, 80°C, leaching for 60min, and centrifuged , taking the supernatant and adding hydrochloric acid aqueous solution, controlling the pH to 5, separating out the precipitate, centrifuging, ...

Embodiment 3

[0040] Embodiment 3: The effect of solid-liquid ratio on protein isolate extraction rate and protein content

[0041] 1. Test method

[0042] In the test, 5 treatment groups were set up according to the mass ratio of the filtrate to the sodium hydroxide solution, and the mass ratios were 1:10, 1:20, 1:30, 1:40, and 1:50. The specific steps of each group of experiments were as follows :

[0043]The defatted flax cake is crushed through a 100-180 mesh sieve, 100g of the sieve is taken, dissolved in 1000g, added with 22g of animal proteolytic compound enzyme, shaken in a water bath at 40-60°C for 120min, filtered, and added to the filtrate Sodium hydroxide solution with a value of 9.5, the mass ratio of the filtrate to the sodium hydroxide solution is 1:10, 1:20, 1:30, 1:40, 1:50, the extraction temperature is 60°C, and the extraction temperature is 60°C. After 60 minutes, centrifuge, take the supernatant and add hydrochloric acid aqueous solution, control the pH to 5, precipit...

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Abstract

The invention relates to a preparation method of flax protein powder. The method comprises the following steps: crushing defatted linseed meal, filtering by a 100-180-mesh sieve, taking siftage, and adding water which is over 10 times of the weight of the siftage to completely dissolve, so as to obtain a flax protein water liquid; adding an animal protein hydrolytic compound enzyme to the flax protein water liquid; oscillating in a water bath at 40-60 DEG C for 120 minutes; determining that the component of which the molecular weight is smaller than 2,000 accounts for 80-83% in flax protein hydrolysate by high performance liquid chromatography, wherein the main component is oligopeptides; filtering, and carrying out alkali dissolution and acid deposition on a filtered product; and freezing and drying the sediment to obtain the flax protein powder. The flax protein is extracted by adopting a method of combining an animal protein hydrolytic compound enzyme technology with an alkali dissolution and acid deposition method, so that the flax protein is separated out to the maximal extent, the extraction rate is 55.67%, and the protein content in the extracted flax protein is 93.77%.

Description

technical field [0001] The invention relates to a preparation method of flax protein powder, belonging to the technical field of edible protein processing methods. Background technique [0002] Flax is one of the main oil crops in my country. Flaxseed is rich in high α-linolenic acid oil, protein, flax glue, dietary fiber and lignans and other nutrients. The well-developed root system of flax can make flax absorb and utilize the nutrition of the earth well, and can store more electronic energy. Therefore, flax contains the most abundant and active electron groups, which can easily absorb oxygen and bind proteins. At present, most domestic linseed processing products are linseed oil prepared from linseed and lignans extracted from linseed cake, and there are few studies on linseed protein peptides. Most of the flax protein at home and abroad is a flax protein isolate extracted from defatted flaxseed or degummed defatted flaxseed by alkali-dissolving and acid-precipitating m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23J3/14A23J3/34
Inventor 王桐于建伟衣洁菡李明乔强徐宏楠
Owner YANTAI NEW ERA HEALTH IND
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