A large yellow croaker fish bone collagen peptide and its preparation method and application

Abstract

The invention discloses a Pseudosciaena crocea fish bone collagen peptide, and a preparation method and application thereof. The amino acid sequence of the collagen peptide is Gly-Phe-Pro-Gly-Ser-Phe-Arg (SEQ ID No:1), and the molecular weight by ESI-MS detection is 823.92Da ([M+H]+824.77Da). The method comprises the following steps: chopping Pseudosciaena crocea fish bone, removing non-collagen substances, carrying out decalcification and degreasing treatment, and preparing fish bone collagen by acid extraction and NaCl salting-out; adding the prepared fish bone collagen into distilled water according to the material-to-liquid ratio of 1g:(15-20mL), regulating the pH value to 1.2-2.0, keeping the temperature at 35-40 DEG C for 5-10 minutes, adding pepsin which accounts for 1.0-1.5 wt% of the collagen, and carrying out enzymolysis for 4-6 hours; regulating the temperature of the enzymolysis solution to 40-50 DEG C, regulating the pH value to 9.0-10.0, keeping the temperature for 5-10 minutes, adding alkaline proteinase which accounts for 1.0-1.5 wt% of the collagen, and carrying out enzymolysis for 3-5 hours; and inactivating the enzyme of the enzymolysis solution by high temperature, centrifuging, ultrafiltering, and carrying out chromatography to obtain the collagen peptide. The preparation technique is scientific and reasonable; the enzymolysis process is easy to control; and the prepared fish bone collagen peptide has the advantages of high oxidation-resistant activity, high safety and no toxic or side effect, and can be used as a medicine, health food or food additive.

Description

technical field [0001] The invention relates to a fish bone collagen peptide and its preparation method and use, in particular to a large yellow croaker fish bone collagen peptide and its preparation method and use. Background technique [0002] Collagen is a macromolecular protein ubiquitous in animals. It mainly exists in the connective tissues (bone, cartilage, skin, tendon, toughness, etc.) of animals, and plays a role in supporting, protecting, combining and forming the body and organs. Boundary and so on. Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity, such as low antigenicity, promotion of cell survival and growth, promotion of platelet aggregation, etc. . Collagen is hydrolyzed under the action of acid, alkali, heat, and enzymes to produce collagen peptides. Compared with collagen, due to the decrease in molecular weight and the increase in wa...

AI Technical Summary

Problems solved by technology

[0004] However, the applicant found that the preparation of large yellow croaker fish bone collagen and collagen peptides using enzymatic hydrolysis technology using large yellow croaker bones as raw materials is still at a blank stage, while the enzymatic hydrolysis products of fish bone collagen are used as materials to prepare antioxidant activity Collagen peptide and its application have not been reported

Images