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Method for producing nisin and lactic acid through simultaneous saccharification and fermentation and application of nisin and lactic acid in feed

A technology of simultaneous saccharification and fermentation and nisin, applied in the field of bioengineering, can solve the problems of high feed cost, easy spoilage of silage, and inability to store for a long time, and achieve the effect of improving feed quality, inhibiting the reproduction of bacteria, and increasing the profit of breeding.

Active Publication Date: 2014-08-20
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main problems faced by the cattle and sheep breeding industry are the high cost of feed, and the silage is easily corrupted and cannot be stored for a long time

Method used

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  • Method for producing nisin and lactic acid through simultaneous saccharification and fermentation and application of nisin and lactic acid in feed
  • Method for producing nisin and lactic acid through simultaneous saccharification and fermentation and application of nisin and lactic acid in feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Get 100ml Erlenmeyer flask, weigh 4% straw respectively and add in the Erlenmeyer flask, then add NaCl 2 g / L, MgSO 4 ·7H 2 O 0 . 2 g / L, yeast extract powder 10 g / L, peptone 10 g / L, CaCO 3 30g / L, then add 2ml pH5 citric acid buffer to the shaker flask and mix well, make up to 27g with deionized water, adjust the pH to 5, seal and weigh for sterilization. After sterilization, place it to dry, weigh it, and calculate the water loss to replenish water. At the same time, add 0.05% Tween-80 to react for 1.5 hours, then add sterilized cellulase 15FPU / g, β-glucosidase 5 IU / g cellulose, Xylanase 5 IU / g cellulose, pectinase 5 IU / g cellulose and shake well, and pre-hydrolyze at 50°C for 3 hours after sealing. After pre-hydrolysis, add 10% inoculum containing lactic acid bacteria to the reaction system Lactococcus lactis subsp. lactis The bacterial solution (OD value 2) was put back in a water bath shaker at 37°C, 160rpm for fermentation, and fermented for 96 hours. After the...

Embodiment 2

[0030] Get 100ml Erlenmeyer flask and weigh 3% straw respectively and add in the Erlenmeyer flask, then add NaCl 2 g / L, MgSO 4 ·7H 2 O 0 . 2 g / L, yeast extract powder 10 g / L, peptone 10 g / L, CaCO 3 50g / L, then add 2ml of pH5 citric acid buffer to the shaker flask and mix well, make up to 27g with deionized water, adjust the pH to 5, seal, weigh and sterilize. After sterilization, place it to dry, weigh it, and calculate the water loss to replenish water. At the same time, add 0.05% Tween-80 to react for 1.5 hours, then add sterilized cellulase 15FPU / g, β-glucosidase 5 IU / g cellulose, Xylanase 5 IU / g cellulose, pectinase 5 IU / g cellulose and shake well, and pre-hydrolyze at 50°C for 3 hours after sealing. After pre-hydrolysis, add 10% inoculum containing lactic acid bacteria to the reaction system Lactococcus lactis subsp. lactisThe bacterial solution (OD value 2) was put back in a water bath shaker at 37°C, 160rpm for fermentation, and fermented for 96 hours. After the f...

Embodiment 3

[0032] Get 100ml Erlenmeyer flask, weigh 4% straw respectively and add in the Erlenmeyer flask, then add NaCl 2 g / L, MgSO 4 ·7H 2 O 0 . 2 g / L, yeast extract powder 10 g / L, peptone 10 g / L, CaCO 3 30g / L, then add 2ml pH5 citric acid buffer to the shaker flask and mix well, make up to 27g with deionized water, adjust the pH to 5, seal and weigh for sterilization. After sterilization, place it to dry, weigh it, and calculate the water loss to replenish water. At the same time, add 0.05% Tween-80 to react for 1.5 hours, then add sterilized cellulase 25FPU / g, β-glucosidase 10 IU / g cellulose, Xylanase 5 IU / g cellulose, pectinase 5 IU / g cellulose and shake well, and pre-hydrolyze at 50°C for 3 hours after sealing. After pre-hydrolysis, add 10% inoculum containing lactic acid bacteria to the reaction system Lactococcus lactis subsp. lactis The bacterial solution (OD value 2) was put back in a water bath shaker at 37°C, 160rpm for fermentation, and fermented for 96 hours. After th...

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Abstract

The invention discloses a method for producing nisin and lactic acid through simultaneous saccharification and fermentation and an application of nisin and lactic acid in a feed. The method comprises the steps of with lignocelluloses as raw materials, hydrolyzing lignocelluloses by using cellulose to form monosaccharide, synchronously converting monosaccharide by using Lactococcus lactis subsp.lactis capable of producing nisin and lactic acid to produce lactic acid and nisin. Lignocelluloses such as crop stalks, rice straw or wood chips and the like are synchronously converted into nisin and lactic acid which both have high additional values; in addition, the produced lactic acid and nisin are used in feed additives for cattle and sheep, so that bacteria in greenfeed can be inhibited from breeding, and the greenfeed cannot be corrupted within 20d; after nisin and lactic acid are used in the feed to breed cattle and sheep, the daily grain is 10% more than that of a control group, the feed is saved by 10%, the breeding profit is increased, meanwhile, the excrement quantities of cattle and sheep are reduced and can be reduced by 5% at most, and the pungent odor is weakened, so that the conditions that the utilization ratio of the feed is increased and the favorable environment-friendly effect is achieved are proved from the side view.

Description

[0001] technical field [0002] The invention discloses a method for synchronous saccharification and fermentation to produce nisin and lactic acid and its application in feed, belonging to the field of bioengineering. technical background [0003] Synchronous saccharification and fermentation are generally used for bioutilization of lignocellulose. Synchronous saccharification and fermentation is a fermentation method in which saccharification and fermentation are carried out at the same time. The glucose released by cellulase is fermented by microorganisms to obtain the final product, thereby eliminating the inhibitory effect of reducing sugar on cellulase products, reducing the amount of cellulase, and reducing cost. Therefore, compared with individual hydrolysis and fermentation of lignocellulosic raw materials, simultaneous saccharification and fermentation have many advantages, and thus are widely used to produce chemical products such as ethanol or lactic acid. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P21/02A23K1/18A23K1/17A23K1/16C12R1/46A23K10/12A23K50/10
Inventor 任晓冬滕利荣刘嘉婧程琪越崔雨潇孟庆繁逯家辉
Owner JILIN UNIV
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