Method for performing colorimetric detection on tetracycline by aptamer and positive electricity AuNPs
A tetracycline and aptamer technology, applied in the field of analytical chemistry, can solve the problems of expensive equipment, high cost, and long measurement time, and achieve the effect of convenient supervision and rapid detection
Inactive Publication Date: 2014-08-20
JILIN UNIV
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Overdose in elderly patients is often accompanied by symptoms such as renal dysfunction, which seriously affects human health and brings major hidden dangers to human health
[0005] The determination methods of tetracycline residues mainly include microbial method, enzyme-linked immunoassay (ELISA), thin-layer chromatography and liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), etc. Although these methods have high sensitivity, However, these methods have the disadvantages of poor specificity. These methods require expensive equipment, long measurement time, high cost, and require professional and technical personnel to operate, so they are not suitable for on-site rapid detection.
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[0022] Preparation of mercaptoethylamine-modified AuNPs
[0023] Materials / Reagents: HAuCl 4 Purchased from Beijing Chemical Reagent Company
[0024] Method: First, a mixture of 400 μL of 0.213M cysteamine hydrochloride and 40 mL of 1.40 mM HAuCl4 was added to a 100 mL round bottom flask. The mixture was stirred for 20 minutes in the dark, and 10 μL of 10 mM freshly prepared NaBH was quickly added 4 The solution was continued to stir for 30 min in the dark. After cooling the obtained solution to room temperature, the precipitate was removed with a 0.4 μm microporous membrane, and the filtrate was placed in a refrigerator at 4° C. for detection.
[0025] Results: The ultraviolet absorption spectrum of AuNPs has a maximum absorption peak at 527nm, which is consistent with the literature reports.
[0026] Since the experimental system is affected by the reaction time, it is necessary to study the influence of the time change of the system.
[0027] Materials / reagents: mercap...
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The invention discloses a method for performing colorimetric detection on tetracycline by an aptamer and positive electricity AuNPs, belonging to the technical field of analytical chemistry. The method is characterized in that the aggregation of AuNPs modified by mercaptoethylamine can be caused by the aptamer, and thus the color of a AuNPs solution is changed from wine red into blue, an absorption peak generates redshift, tetracycline and the aptamer firstly act and then form a tetracycline-aptamer composition without causing AuNPs aggregation, and the residual quantity of tetracycline in food can be detected by naked eyes and an ultraviolet spectrophotometer. The method is capable of simply, rapidly and sensitively detecting tetracycline, and provides convenience for subsequent research, production, monitoring and the like.
Description
technical field [0001] The method for colorimetrically detecting tetracycline by using an aptamer and positively charged AuNPs belongs to the technical field of analytical chemistry. Background technique [0002] Tetracyclines are broad-spectrum antimicrobials and are the cheapest of antibiotics. Commonly used tetracyclines include tetracycline, aureomycin, oxytetracycline, and doxycycline. [0003] Among tetracycline drugs, tetracycline is widely used, and its low price makes tetracycline very popular in developing countries. It is not only widely used as a drug to treat human and animal diseases, but also used as an additive in large quantities to prevent and treat animal diseases. Poultry diseases, used to improve feed utilization efficiency and promote animal growth. However, due to the unreasonable use and abuse of such drugs, it is easy to induce drug-resistant strains, and it will also lead to the problem of tetracycline residues in food and milk. Both the European ...
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IPC IPC(8): G01N21/33
Inventor 孙春燕罗叶丽郭佳佳李莹曹先一许景月沈斐
Owner JILIN UNIV