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Multiplication subculture culture medium for cowberry blueberries

A subculture medium and micronutrient technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as constraints on development and popularization, loss of excellent characteristics of lingonberry, susceptibility to fungal diseases, etc. , to achieve the effect of fast reproduction speed, not easy to be infected with fungal diseases, good promotion and application value

Inactive Publication Date: 2014-09-03
蚌埠禹辰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to the loss of the inherent good characteristics of lingonberry, which to a certain extent restricts the development and popularization of this tree species. , and in the process of cultivation, especially in the process of multiplication and subculture, it is necessary to carry out pest-free management and control the multiplication rate to shorten the multiplication cycle and increase the survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0012] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,

[0013] Wherein, the components of the macronutrient elements and their corresponding concentrations are: potassium nitrate 190 mg / L; ammonium sulfate 165 mg / L; magnesium sulfate heptahydrate 350 mg / L; calcium nitrate tetrahydrate 680 mg / L; anhydrous phosphoric acid Potassium dihydrogen 100mg / L; disodium edetate 73.4mg / L; ferrous sulfate heptahydrate 18.5 mg / L.

[0014] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 15.6mg / L; zinc sulfate 2.0mg / L; boric acid 3.5 mg / L; potassium iodide 0.5mg / L; sodium molybdate 0.3mg / L L; cobalt chloride 0.05 mg / L, copper sulfate 0.02 mg / L,

[0015] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 0.1 mg / L; nicotinic acid 1.5 mg / L; pyridoxine hydrochloride 1.5 mg / L; inositol 100.0 mg / L.

Embodiment 1

[0017] Proliferation subculture medium for lingonberry, including modified MS + 1.0mgL -1 BA+1.0mgL -1 ZT + 0.1mgL -1 NAA + 1.0gL -1 Agar + 10gL -1 Sucrose, and the pH value is 5.2.

Embodiment 2

[0019] Proliferation subculture medium for lingonberry, including modified MS + 2.0mgL -1 BA+2.0mgL -1 ZT + 0.2mgL -1 NAA + 2.0gL -1 Agar + 10gL -1 Sucrose, and the pH value is 5.2.

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PUM

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Abstract

The invention discloses a multiplication subculture culture medium for cowberry blueberries. The multiplication subculture culture medium is characterized by comprising improved MS, 1.0-2.0mg / L of BA, 1.0-2.0mg / L of ZT, 0.1-0.2mg / L of NAA, 1.0-2.0g / L of agar and 10g / L of sucrose; the pH value is 5.2. According to the multiplication subculture culture medium for the cowberry blueberries, components and contents are improved and a reasonable culture method is adopted so that the reproduction speed of explants of the cowberry blueberries is high, the reproduction period is short and the survival rate is high; the explants of the cowberry blueberries are not easily infected with fungous diseases and have high fine breed degree so that the finally-cultivated cowberry blueberries have good popularization and application values.

Description

technical field [0001] The invention relates to a lingonberry proliferation subculture medium, which belongs to the technical field of botany. Background technique [0002] There are many ways suitable for lingonberry propagation, such as cutting propagation, rhizome cutting propagation, branch propagation and seed propagation. The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to the loss of the inherent good characteristics of lingonberry, which restricts the development and popularization of this tree species to a certain extent. , and in the process of cultivation, especially in the process of multiplication and subculture, it is necessary to carry out pest-free management and control the multiplication rate to shorten the multiplication cycle and increase the survival rate. Contents of the invention [0003] In order to ov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赵兰
Owner 蚌埠禹辰科技有限公司