A kind of tissue culture method of cranberry
A technology of tissue culture and culture medium, applied in the field of botany, can solve problems such as restricting the development and popularization of tree species, loss of excellent characteristics of lingonberry, susceptibility to fungal diseases, etc., to achieve good promotion and application value, and not easy to be infected with fungal diseases Effects of diseases and rapid reproduction
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[0020] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,
[0021] Wherein, the components of the macronutrient elements and their corresponding concentrations are: potassium nitrate 190mg / L; ammonium sulfate 165mg / L; magnesium sulfate heptahydrate 350mg / L; calcium nitrate tetrahydrate 680mg / L; Potassium hydrogen 100mg / L; disodium edetate 73.4mg / L; ferrous sulfate heptahydrate 18.5mg / L.
[0022] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 15.6mg / L; zinc sulfate 2.0mg / L; boric acid 3.5mg / L; potassium iodide 0.5mg / L; sodium molybdate 0.3mg / L L; cobalt chloride 0.05mg / L, copper sulfate 0.02mg / L,
[0023] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 0.1 mg / L; nicotinic acid 1.5 mg / L; pyridoxine hydrochloride 1.5 mg / L; inositol 100.0 mg / L.
Embodiment 1
[0025] A method for tissue culture of lingonberry, comprising the following steps:
[0026] (1) Material selection: In March, select the vigorously growing semi-lignified young shoots, wash them with clean water for 3 times, blot the water with filter paper, and then use 0.1% mercuric chloride on the clean bench to clean the new shoots. After sterilizing for 5 minutes, wash with sterile water for 3 times, then filter paper to dry the water and set aside;
[0027] (2) Bud initiation culture: cut 1-2cm stem segment with a full bud from the new shoot treated in step (1) to form an explant, and insert the base of the explant into the bud initiation medium Under the conditions of temperature 25-30°C, light intensity 2000-2500lx, and light time 12h / d, culture for 30 days to form new shoots, stem axillary buds start to germinate, stem tips elongate significantly, and the buds are about 50 days long. into about 3cm new shoots, the bud initiation medium includes: improved MS+0.05mgL ...
Embodiment 2
[0032] A method for tissue culture of lingonberry, comprising the following steps:
[0033] (1) Material selection: In May, select the semi-lignified young shoots that grow robustly, wash them with water for 5 times, and dry them with filter paper. Then, use 0.1% mercury chloride on the clean workbench After sterilizing for 10 minutes, wash with sterile water for 5 times, then dry the water with filter paper and set aside;
[0034] (2) Bud initiation culture: cut 1-2cm stem segment with a full bud from the new shoot treated in step (1) to form an explant, and insert the base of the explant into the bud initiation medium , under the conditions of temperature 25-30°C, light intensity 2000-2500lx, and light time 12h / d, culture for 40 days to form new shoots. The bud initiation medium includes: improved MS+0.1mgL -1 BA+2.0mgL -1 ZT+2.0gL -1 Agar+10gL -1 Sucrose, and the pH value is 5.2;
[0035] (3) Proliferation and subculture: Inoculate the new branches described in step (2...
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