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A kind of proliferation subculture method of kiwifruit

A subculture, kiwifruit technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as restricting the vigorous development and popularization of kiwifruit, reducing the quality and nutritional value of kiwifruit, and being susceptible to fungal diseases. , to achieve the effect of being conducive to vigorous development and popularization and application, less susceptible to fungal diseases, high fruit quality and nutritional value

Inactive Publication Date: 2016-03-02
重庆三磊田甜农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to a reduction in the fruit quality and nutritional value of kiwifruit, and a decrease in taste, which to a certain extent restricts the vigorous development of kiwifruit. Develop and promote applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0018] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,

[0019] And the component of described macronutrient element and its corresponding concentration are: potassium nitrate 1900mg / L; Ammonium sulfate 1650mg / L; Magnesium sulfate heptahydrate 350mg / L; Dihydrate calcium chloride 400mg / L; Potassium hydrogen 150mg / L; disodium edetate 35.6mg / L; ferrous sulfate heptahydrate 25.8mg / L.

[0020] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 20.5mg / L; zinc sulfate 9.0mg / L; boric acid 6.5mg / L; potassium iodide 0.5mg / L; sodium molybdate 0.3mg / L L; cobalt chloride 0.05mg / L, copper sulfate 0.02mg / L,

[0021] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 10.0 mg / L; nicotinic acid 1.5 mg / L; pyridoxine hydrochloride 1.5 mg / L; inositol 80.0 mg / L.

Embodiment 1

[0023] A method for the proliferation and subculture of kiwifruit, comprising the following steps:

[0024] (1) Material selection: Select the vigorously growing basal branches and wash them with clean water for 2 hours, then spray the selected branches with 5% carbendazim, and use filter paper to absorb the moisture on the surface of the stems for later use;

[0025] (2) Disinfection of stem segments and germination initiation of buds: place the stem segments treated in step (1) on an ultra-clean workbench, disinfect with 75% alcohol for 10 seconds, then wash with sterile water for 3 times, and then rinse with 0.1 % mercuric chloride sterilized and soaked for 5 minutes, and finally rinsed with sterile water for 3 times, blotted dry with filter paper, cut 1-2 cm stem section with a full bud, and then inserted the base of the stem section into the bud initiation medium Above, inoculate 3 explants in each bottle, induce terminal buds and axillary buds to germinate, and cultivate...

Embodiment 2

[0031] A method for the proliferation and subculture of kiwifruit, comprising the following steps:

[0032] (1) Material selection: Select the vigorously growing basal branches and wash them with water for 3 hours, then spray the selected branches with 5% carbendazim, and use filter paper to absorb the water on the surface of the stems for later use;

[0033] (2) Disinfection of stem segments and germination initiation of buds: put the stem segments treated in step (1) on the ultra-clean workbench, disinfect with 75% alcohol for 15 seconds, then wash with sterile water for 5 times, and then rinse with 0.1 % mercuric chloride sterilized and soaked for 8 minutes, and finally rinsed with sterile water for 5 times, blotted dry with filter paper, cut 1-2 cm stem section with a full bud, and then inserted the base of the stem section into the bud initiation medium Above, 5 explants were inoculated in each bottle to induce the germination of terminal buds and axillary buds. Under the...

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PUM

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Abstract

The invention discloses a method for the proliferation and subculture of kiwifruit, which comprises the following steps: (1) material selection; (2) stem section disinfection and bud germination culture; (3) aseptic tube seedling proliferation culture; (4) rooting culture ; (5) Hardening and transplanting. The proliferation and subculture method of kiwifruit of the present invention improves the basic medium and components, and assists a reasonable culture method at the same time, so that the explants of kiwifruit have a fast propagation speed, a short reproduction cycle, a high reproduction rate, a high survival rate, and are not easy to produce. Infected with fungal diseases, and the degree of improved varieties is high, so that the fruit quality and nutritional value of the finally cultivated kiwifruit are high, and the taste is good, which is conducive to the vigorous development and popularization of the kiwifruit.

Description

technical field [0001] The invention relates to a method for multiplying and subculturing kiwifruit, belonging to the technical field of botany. Background technique [0002] Adventitious roots are easy to be produced on the stems of kiwifruit, and adventitious buds are easy to be produced on the stems and branches of kiwifruit. There are many ways suitable for kiwifruit propagation, such as cutting propagation, layering propagation, grafting propagation and seed propagation. The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to a reduction in the fruit quality and nutritional value of kiwifruit, and a decrease in taste, which to a certain extent restricts the vigorous development of kiwifruit. Develop and promote applications. Contents of the invention [0003] In order to overcome the deficiencies of the prior art, the purpos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵兰
Owner 重庆三磊田甜农业开发有限公司