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A kind of proliferating subculture medium of kiwifruit

A subculture medium and kiwifruit technology, applied in the field of botany, can solve the problems of kiwifruit fruit quality and nutritional value reduction, constraints on the vigorous development and popularization of kiwifruit, susceptibility to fungal diseases, etc. The effect of high fruit quality and nutritional value and fast reproduction speed

Inactive Publication Date: 2016-03-02
蚌埠禹辰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to a reduction in the fruit quality and nutritional value of kiwifruit, and a decrease in taste, which to a certain extent restricts the vigorous development of kiwifruit. Develop and promote applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0012] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,

[0013] And the component of described macronutrient element and its corresponding concentration are: potassium nitrate 1900mg / L; Ammonium sulfate 1650mg / L; Magnesium sulfate heptahydrate 350mg / L; Dihydrate calcium chloride 400mg / L; Potassium hydrogen 150mg / L; disodium edetate 35.6mg / L; ferrous sulfate heptahydrate 25.8mg / L.

[0014] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 20.5mg / L; zinc sulfate 9.0mg / L; boric acid 6.5mg / L; potassium iodide 0.5mg / L; sodium molybdate 0.3mg / L L; cobalt chloride 0.05mg / L, copper sulfate 0.02mg / L,

[0015] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 10.0 mg / L; nicotinic acid 1.5 mg / L; pyridoxine hydrochloride 1.5 mg / L; inositol 80.0 mg / L.

Embodiment 1

[0017] Proliferation subculture medium of kiwifruit, including improved MS+1.0mgL -1 BA+1.0mgL -1 KT+0.1mgL -1 NAA+3.0gL -1 phytagel+2mgL -1 GA 3 +20gL -1 Sucrose, and the pH value is 5.8.

[0018] Utilize the kiwifruit proliferation subculture medium described in Example 1 to carry out proliferation culture to the kiwifruit explants, the frequency of proliferation subculture is subculture once every 2 weeks, and its new seedlings grow by the ratio of 1:15-18.

Embodiment 2

[0020] Proliferation subculture medium of kiwifruit, including improved MS+2.0mgL -1 BA+1.0~1.5mgL -1 KT+0.3mgL -1 NAA+3.5gL -1 phytagel+3mgL -1 GA 3 +20gL -1 Sucrose, and the pH value is 5.8.

[0021] Utilize the kiwifruit proliferation subculture medium described in Example 1 to carry out proliferation culture to the kiwifruit explants, the frequency of proliferation subculture is subculture once every 2.5 weeks, and its new seedlings grow by the ratio of 1:16-18.

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PUM

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Abstract

The invention discloses a kiwi fruit proliferation subculture medium, which is characterized in that it comprises improved MS+1.0~2.0mgL-1BA+1.0~1.5mgL-1KT+0.1~0.3mgL-1NAA+3.0~3.5gL-1phytagel+ 2~3mgL-1GA3+20gL-1 sucrose, and the pH value is 5.8. The kiwifruit proliferation subculture medium of the present invention improves the basic medium and components, so that the explants of kiwifruit have a fast propagation speed, a short reproduction cycle and a high reproduction rate, are not easily infected with fungal diseases, and have a high degree of improved variety. Therefore, the fruit quality and nutritional value of the finally cultivated kiwi fruit are high, and the taste is good, which is conducive to the vigorous development and popularization of the kiwi fruit.

Description

technical field [0001] The invention relates to a kiwi fruit proliferation subculture medium, which belongs to the technical field of botany. Background technique [0002] Adventitious roots are easy to be produced on the stems of kiwifruit, and adventitious buds are easy to be produced on the stems and branches of kiwifruit. There are many ways suitable for kiwifruit propagation, such as cutting propagation, layering propagation, grafting propagation and seed propagation. The above-mentioned breeding method has the advantages of long breeding cycle, susceptibility to fungal diseases, and reduced degree of improved varieties, while the use of inferior seedlings leads to a reduction in the fruit quality and nutritional value of kiwifruit, and a decrease in taste, which to a certain extent restricts the vigorous development of kiwifruit. Develop and promote applications. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the object of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 赵兰
Owner 蚌埠禹辰科技有限公司