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A kind of continuous reaction hydrogen production method of photosynthetic bacteria

A photosynthetic bacteria and reaction technology, which is applied in the field of continuous reaction of photosynthetic bacteria to produce hydrogen, can solve the problems such as the reduction of the hydrogen production efficiency of photosynthetic bacteria and the conversion rate of substrates, the large pressure difference between the gas collecting pipeline and the reactor, and the difficulty of cleaning the reactor. , to achieve the effect of improving substrate utilization, increasing efficiency and ensuring stability

Inactive Publication Date: 2015-12-02
TAIYUAN UNIV OF TECH
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Problems solved by technology

However, after the reactor has been used for a period of time, some photosynthetic bacteria will float on the inner wall of the reactor and affect the light transmission of the reactor. Therefore, the reactor needs to be cleaned regularly to ensure its light transmission.
However, the reactor of this invention is a whole and cannot be disassembled, and the internal baffles increase the difficulty of cleaning the reactor
[0007] In addition, the current photosynthetic biological hydrogen production reaction device cannot prevent the culture medium in the reactor from being polluted by miscellaneous bacteria. Under the influence of miscellaneous bacteria, the hydrogen production efficiency and substrate conversion rate of photosynthetic bacteria will be reduced.
Moreover, the existing photosynthetic biological hydrogen production reaction device cannot solve the following two problems that exist when collecting the generated hydrogen
1. If the generated hydrogen is not compressed and stored, a huge hydrogen storage device is required; however, when the generated hydrogen is compressed and stored, the internal pressure of the gas collecting pipeline between the hydrogen production reactor and the compression pump drops, causing the gas collecting pipe There is a large pressure difference between the pipeline and the reactor, so that the culture solution in the reactor flows into the gas collection pipeline

Method used

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  • A kind of continuous reaction hydrogen production method of photosynthetic bacteria
  • A kind of continuous reaction hydrogen production method of photosynthetic bacteria
  • A kind of continuous reaction hydrogen production method of photosynthetic bacteria

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Embodiment Construction

[0033] First, through the injection port 40 of the incubator 44 in the photosynthetic bacteria culture device 7 after sterilization, the photosynthetic bacteria liquid and the growth medium with a volume ratio of 1:4 are injected in the incubator 44 to carry out proliferation and cultivation. Bacteria concentration OD in the incubator 660nm After reaching 1.6 to 1.9, pump 45 to transport 4 / 5 to 5 / 6 of the bacterial solution by volume into the concentration tank 46, and pass through the concentration tank 46, which is 4 / 5 to 5 / 6 box length away from the bacterial solution inlet Concentrate the microporous membrane III39 with a pore size ≤ 0.22 μm, and stop concentrating when the concentration of the bacterial solution reaches 2 to 3 times the concentration of the original bacterial solution. The separated culture solution is returned to the incubator 44 through the liquid inlet 43, and new culture solution is added through the injection port 40 to continue to proliferate and cu...

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Abstract

The invention relates to a hydrogen manufacturing method through a photosynthetic bacterium continuous reaction. According to the hydrogen manufacturing method through the photosynthetic bacterium continuous reaction, a photosynthetic bacterium continuous hydrogen manufacturing reaction device is adopted for thallus culturing and hydrogen manufacturing through the reaction. The device is composed of a reactor, a raffinate treating pond, a hydrogen collecting device, a high-concentration hydrogen generating culture solution box and a photosynthetic bacterium culturing device; according to thallus culturing and hydrogen manufacturing through the reaction, after photosynthetic bacteria are cultured in a multiplication mode till OD 660 nm reaches 1.6-1.9, four fifths to five sixths of a bacterium solution by volume is concentrated through a millipore filtering membrane III with the pore diameter smaller than or equal to 0.22 micrometer, the concentrated bacterium solution is injected into the reactor for photosynthetic hydrogen generation under regulation and control of a computer, and the bacterium solution not concentrated is added with a new culture solution and then continues to be cultured in a multiplication mode. A substrate is recycled, and other bacteria can not enter a reactor body due to membrane filtration, so that the influence on photosynthetic bacterium hydrogen generation by the other bacteria is avoided, and hydrogen generation efficiency and the use ratio of the substrate are improved.

Description

technical field [0001] The invention relates to a method for continuous reaction hydrogen production by photosynthetic bacteria, in particular to a method for cultivating bacteria and producing hydrogen by reaction using a photosynthetic bacteria continuous hydrogen production reaction device. Background technique [0002] With the continuous consumption of fossil energy and the increasingly serious environmental pollution caused by its long-term use, human beings will face a very serious energy crisis and environmental degradation. Hydrogen has a high calorific value, fast combustion reaction, and produces water after releasing energy and is renewable, so it is recognized as a clean and green energy. At present, the methods of hydrogen production mainly include electrolysis of water to produce hydrogen, chemical hydrogen production, biological hydrogen production, etc. Compared with other hydrogen production methods, biological hydrogen production has the advantages of low ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/12C12M1/02C12M1/107
CPCC12M21/02C12M21/04C12M23/18C12M29/04
Inventor 吕永康刘玉香孙明星
Owner TAIYUAN UNIV OF TECH
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