Screening and domestication method of bacteria colony for producing polyhydroxyalkanoate by using xylose

Abstract

The invention provides a screening and domestication method of a bacteria colony for producing polyhydroxyalkanoate by using xylose, and aims at obtaining a mixed bacteria colony for producing PHA by using xylose at one time. A satiation hunger domestication mode of a PHA synthesis bacteria colony is adopted, a sludge sample is domesticated by taking xylose as a sole carbon source, and in the domestication process, a Nile blue staining method of the polyhydroxyalkanoate synthesis bacteria colony is performed to screen the bacteria colony and the bacteria colony is refilled to the bacteria colony domesticated by using xylose. The stable high-yielding PHA mixed bacteria colony is finally obtained, and the mixed bacteria colony system mainly contains four dominant strains, namely, gamma-Proteobacteria, Cellvibrio sp., Uncultured bacterium and Pseudomonas putida. The method is simple and effective and short in domestication time, the obtained bacteria colony has a relatively high capability of producing PHA by using xylose, and great significance and practical values in producing PHA by using a cellulose base raw material in a fermentation mode are achieved.

Description

technical field [0001] The invention relates to a screening and acclimation method for efficiently utilizing xylose to produce polyhydroxyalkanoate flora. Background technique [0002] Polyhydroxyalkanoates (polyhydroxyalkanoates PHA) are carbon source storage particles synthesized by many bacteria under unbalanced growth conditions, and are favored as a renewable and biodegradable plastic. In addition to its properties similar to traditional plastics, it also has the characteristics of biocompatibility and optical activity. It has become a good substitute for plastics and has great potential in solving environmental pollution and energy shortages. At present, PHA is mainly produced by fermentation of pure bacteria, including Alcaligenes, nitrogen-fixing bacteria, Pseudomonas and recombinant Escherichia coli. However, pure bacterial fermentation requires the use of high-quality substrates such as glucose, as well as fermentation control processes such as sterilization, resu...

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Problems solved by technology

However, there is a lack of suitable domestication and screening pressure for PHA synthetic strains, and the traditional satiety-starvation mode and anaerobic aerobic mode have a long domestication period, and require small molecular fatty acids as domestication substrates, so there is currently no use of mixed flora A report on the production of PHA from xylose

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