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A kind of albumin indocyanine green paclitaxel complex and its preparation method and application

A technology of paclitaxel complex and indocyanine green, which is applied in the field of albumin indocyanine green paclitaxel complex and its preparation, can solve the problem of low accumulation in tumor sites, poor stability of ICG and albumin binding, Blood circulation time is not very good and other problems, to achieve good water solubility and biocompatibility, improve blood circulation time, good optical absorption effect

Active Publication Date: 2017-05-24
SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the binding stability of ICG and albumin is not very good, and the blood circulation time is not very good, and the enrichment at the tumor site is not high

Method used

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  • A kind of albumin indocyanine green paclitaxel complex and its preparation method and application
  • A kind of albumin indocyanine green paclitaxel complex and its preparation method and application
  • A kind of albumin indocyanine green paclitaxel complex and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of albumin indocyanine green paclitaxel complex:

[0056] With albumin (HSA) as the substrate, indocyanine green and paclitaxel are adsorbed through hydrophobic interaction, and paclitaxel can be used as a cross-linking agent to make albumin self-assemble into large nanoparticles, forming albumin indocyanine green paclitaxel complex (HSA- ICG-PTX). The specific preparation method is as follows:

[0057] Dissolve indocyanine green (ICG) in dimethyl sulfoxide solution to make a 10 mg / mL indocyanine green solution, and dissolve paclitaxel (PTX) in ethanol to make a 10 mg / mL paclitaxel solution; Take 2 mg of human serum albumin (HSA), dissolve it in 2 mL of phosphate buffered saline solution, and prepare a 1 mg / mL phosphate buffered saline solution of human serum albumin;

[0058] Indocyanine green solution and paclitaxel solution are added to the phosphate buffer solution of human serum albumin in different proportions to obtain albumin indocyan...

Embodiment 2

[0060] Embodiment 2, the preparation of albumin indocyanine green complex

[0061] Dissolve indocyanine green (ICG) in dimethyl sulfoxide solution to form a 10 mg / mL indocyanine green solution; weigh 2 mg of human serum albumin (HSA), dissolve it in 2 mL of phosphate buffered saline, Then add 6 μL of indocyanine green solution, and stir overnight (12h) in the dark; finally, the mixture obtained after stirring overnight is centrifuged at 14800rpm for 5min to remove unreacted ICG, and the supernatant is albumin-indocyanine green complex ( HSA-ICG).

Embodiment 3

[0062] Embodiment 3, preparation of albumin-paclitaxel complex

[0063] Adding the paclitaxel solution in different proportions to the phosphate buffer solution of human serum albumin can obtain the albumin-paclitaxel complexes in different proportions.

[0064] Among them, paclitaxel (PTX) was dissolved in ethanol to prepare a 10 mg / mL paclitaxel solution; 2 mg of human serum albumin (HSA) was weighed, dissolved in 2 mL of phosphate buffered saline solution, and then 25 μL of paclitaxel solution was added and stirred Overnight (12h); Finally, the mixture obtained after stirring overnight was centrifuged at 14800rpm for 5min to remove unreacted PTX, and the supernatant was 0.2mg / ml nab-paclitaxel complex (HSA-PTX).

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Abstract

An albumin indocyanine green and paclitaxel compound, consisting of indocyanine green, paclitaxel and human serum albumin. The preparation method comprises adsorbing indocyanine green and paclitaxel onto human serum albumin by hydrophobic force. The compound is used to prepare drugs for treating cancer, and to conduct photo-thermal treatment and chemotherapy in fluorescence imaging mode.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an albumin indocyanine green paclitaxel complex and a preparation method and application thereof. Background technique [0002] Cancer is one of the major malignant diseases that threaten human health. Although a large amount of human, material and financial resources have been invested in the prevention and treatment of cancer for decades since the 1950s, the progress made by human beings in this regard is very limited. [0003] Paclitaxel is a widely used anticancer drug after doxorubicin and cisplatin. It is mainly suitable for ovarian cancer and breast cancer, and it is also effective for lung cancer, colorectal cancer, melanoma, head and neck cancer, lymphoma, and brain tumor. All showed positive clinical efficacy. However, because paclitaxel is highly insoluble in water, polyoxyethylene castor oil and absolute ethanol are needed as co-solvents, and polyoxyethylene castor oil wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K41/00A61K31/337A61K47/42A61P35/00
CPCA61K31/337A61K41/00A61K47/42A61K49/00
Inventor 陈倩刘庄
Owner SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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