Method for screening of anti-porcine reproductive and respiratory syndrome pigs based on mannose receptor MRC-1 gene expression quantity

Abstract

The invention provides a method for screening of anti-porcine reproductive and respiratory syndrome (PRRS) pigs based on mannose receptor (MRC-1) gene expression quantity. The method designs fluorescence real-time quantitative PCR primers of porcine reproductive and respiratory syndrome virus (PRRSV) infection closely related genes, the primers are employed to detect the normal expression level of MRC-1 gene in pigs before infection, and then the expression level of the gene after 5 days of infection is detected. If the expression quantity of the MRC-1 significantly increases, pigs of the variety have strong resistance to PRRSV and belong to the anti-PRRS pig variety, thus being conducive to breeding of excellent line pigs.

Description

technical field [0001] The invention relates to molecular biology detection technology of pigs resistant to PRRS, in particular to a method for screening pigs resistant to PRRS based on the expression level of mannose receptor MRC-1 gene. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "PRRS", is caused by porcine reproductive and respiratory syndrome virus (PRRSV), a member of the family Arteriviridae of the order Nidoviridae. There are equine arteritis virus, murine lactate dehydrogenase virus, and simian hemorrhagic fever virus. The disease mainly causes reproductive and respiratory symptoms of pigs, manifested as interstitial pneumonia and mummified sow abortion, which causes huge economic losses to the pig industry all over the world every year. A high fever outbreak occurred in China in 2007. The disease spread rapidly across the country. More than 2 million pigs were infected and 400,000 pigs died. The disease was...

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Problems solved by technology

PRRSV mainly infects alveolar macrophages of pigs. Now studies have shown that after PRRSV infection, PRRSV infection in innate immunity cannot elicit an effective type I interferon response, humoral immunity cannot produce effective neutralizing antibodies in time, and antibody-dependent Virus enhancement effect eventually leads to immunosuppression and persistent infection. Due to the extremely high mutation rate of the virus, traditional vaccine methods cannot effectively control the disease. Many studies are devoted to finding new methods such as RNAi and interferon to target the virus. Used to make up for the shortcomings of traditional methods

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