Method and reagent kit for measuring activated partial thromboplastin time

A thromboplastin time and kit technology, which is applied in biological testing, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as prolonged clotting time, and achieve the effect of inhibiting white turbidity or precipitation

Inactive Publication Date: 2014-10-01
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] On the other hand, it is known that when LA, which is a kind of antiphospholipid antibody, is contained in the blood sample obtained from the test subject in the measurement using the reagent for APTT measurement as described above, since LA inhibits the blood coagulation necessary Phospholipids, prolonged clotting time

Method used

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  • Method and reagent kit for measuring activated partial thromboplastin time
  • Method and reagent kit for measuring activated partial thromboplastin time
  • Method and reagent kit for measuring activated partial thromboplastin time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Examination of the effect of adding phosphatidylglycerol on the sensitivity of LA

[0048] (1-1) Preparation of reagents for APTT measurement

[0049] A conventional APTT measurement reagent (reagent A) not containing PG was prepared. In addition, white turbidity and precipitation were not confirmed in the reagent A.

[0050]

[0051] Reagent A, containing 0.1 mmol / L ellagic acid (Kishida Chemical Co., Ltd.) as an activator, 140 μg / mL L-α-phosphatidylethanolamine (Avanti Polar Lipids, Inc.) as a phospholipid, 160 μg / mL L-α- Phosphatidylcholine (Avanti Polar Lipids, Inc.) and 40μg / mL L-α-phosphatidylserine (Avanti Polar Lipids, Inc.), as a buffer containing 50mM HEPES buffer (pH7.35), as a preservative containing 0.1% sodium azide, containing 3-t-butyl-4-hydroxyanisole (Nacalai tesque Co., Ltd.) as an antioxidant.

[0052] As reagents of the present invention, PG-containing reagents for measuring APTT (reagents B to E) were prepared. In addition, no whit...

Embodiment 2

[0069] Example 2: Examination of the Effect of Modulation of Phospholipid Composition on the Sensitivity of LA

[0070] (2-1) Preparation of reagents for APTT measurement

[0071] Reagents for APTT measurement (reagents F to N) containing PE, PC, and PS at various concentrations were prepared without PG. In addition, any reagent contained 0.1 mmol / L ellagic acid (Kishida Chemical Co., Ltd.) as an activator, 50 mM HEPES buffer (pH 7.35) as a buffer, and 0.1% sodium azide as a preservative. Contains 3-t-butyl-4-hydroxyanisole (Nacalai Tesque Co., Ltd.) as an antioxidant. The phospholipid composition of each reagent is shown next.

[0072]

[0073] Reagents F to J all contained 140 μg / mL L-α-phosphatidylethanolamine (Avanti Polar Lipids, Inc.) and 160 μg / mL L-α-phosphatidylcholine (Avanti Polar Lipids, Inc.), at different concentrations Contains L-alpha-phosphatidylserine (Avanti Polar Lipids, Inc.). That is, reagents F to J contained L-α-phosphatidylserine at concentration...

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Abstract

The present invention provides a method for measuring activated partial thromboplastin time. The method comprises: a first mixing step of mixing a blood plasma with a first reagent, wherein the first reagent comprises an activator and phosphatidylglycerol at a concentration equal to or greater than 25 µg / mL; a second mixing step of mixing a sample obtained in the first mixing step with a second reagent comprising a calcium salt; and a step of measuring coagulation time of the sample obtained in the second mixing step.

Description

technical field [0001] The present invention relates to a method for measuring activated partial thromboplastin time (APTT) and a kit for measuring APTT. Background technique [0002] In the field of clinical examinations, examinations for studying abnormalities of blood coagulation factors and the like by measuring blood coagulation time are known. One of the general clinical tests of blood coagulation ability includes APTT measurement. APTT refers to the coagulation time that reflects the function of the intrinsic coagulation pathway of blood initiated by contact with a negatively charged foreign body during bleeding. Measurement of APTT is used not only for screening tests for the deficiency or abnormality of intrinsic coagulation factors, but also for monitoring heparin therapy and the like. [0003] APTT measurement has long been established as a clotting time measurement method utilized in clinical examinations. In this method, a negatively charged activator, a phos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N2405/04C12Q1/56G01N33/86
Inventor 奥田昌宏木下英美铃木健史
Owner SYSMEX CORP
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