Method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco

A technology of solanesol and rutin, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of unsatisfactory extraction process, high extraction temperature, and low extraction rate, so as to reduce energy consumption, Efficient extraction and simple operation

Active Publication Date: 2014-10-08
TOBACCO RES INST CHIN AGRI SCI ACAD
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AI Technical Summary

Problems solved by technology

However, at present, the research and utilization of tobacco functional components are mainly based on the extraction and utilization technology of a single functional component. Although the comprehensive extraction technology of different functional components has begun to receive attention, it is mainly the comprehensive extraction technology of solanesol and nicotine. There are few reports on the comprehensive extraction technology of substances. Ai Xinxin of Jiangnan University reported the technology of simultaneously extracting tobacco solanesol, chlorogenic acid, and alkaloids with 95% ethanol ultrasonic assistance, and the extraction rates were 82% and 94% respectively. , 95%, the extract

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  • Method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco
  • Method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco

Examples

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Embodiment 1

[0021] Such as figure 1 A method for simultaneously extracting and separating tobacco chlorogenic acid, solanesol, nicotine, and rutin is shown, and its steps include:

[0022] 1. Simultaneous extraction of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0023] Weigh 500g of the pulverized tobacco powder sample, extract once with 80% ethanol at a solid-liquid ratio of 1:10, ultrasonic frequency 45 KHz, extraction temperature 40-50°C, and then use 80% and 95% ethanol to remove the residue Each extraction was performed once for 20 minutes; the obtained extracts were combined to obtain extracts of tobacco chlorogenic acid, solanesol, nicotine, and rutin, and the extraction rates of the four components all reached 100%.

[0024] 2. Separation of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0025] 1. Separation of solanesol

[0026] The extract obtained in step 1 was concentrated under reduced pressure at 50°C until there was no alcohol smell, and the eth...

Embodiment 2

[0033] Such as figure 2 As shown, the difference from Example 1 is:

[0034] 2. Separation of tobacco chlorogenic acid, solanesol, nicotine and rutin

[0035] 1. Separation of solanesol

[0036] The extract was concentrated under reduced pressure at 50°C until there was no alcohol smell, and ethanol was recovered. After the concentrated solution is cooled, it is quickly filtered, solanesol remains in the precipitate, and chlorogenic acid, rutin, and nicotine remain in the filtrate. Wash the precipitate with 20-40% ethanol solution, dissolve it in absolute ethanol, add 30% sodium hydroxide solution, saponify at 40-50°C under ultrasonic conditions for 40min, add deionized water after saponification, and then extract with petroleum ether 3 times, the extracts were combined and concentrated to obtain crude solanesol. The crude solanesol is refined by column chromatography, preparative chromatography, crystallization and other methods to obtain pure solanesol.

[0037] 2. Se...

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Abstract

The invention relates to a method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco. The method comprises the following steps: 1, extracting simultaneously, namely, crushing a tobacco raw material, extracting by using 80% ethanol once under the conditions that the temperature is 40-50 DEG C, the ultrasonic frequency is 45KHz and the ratio of material to liquid is 10:1, and performing suction filtration or centrifugation, respectively extracting the residue by using 80% and 95% ethanol once; combining the extract solutions of three times, thereby obtaining crude extract of chlorogenic acid, solanesol, alkaloid and rutin in tobacco, wherein the extraction rates of chlorogenic acid, solanesol, alkaloid and rutin in tobacco are 100%; and 2, separating, namely, separating solanesol, separating chlorogenic acid and rutin, and separating alkaloid. By adopting the method, simultaneous and efficient extraction on chlorogenic acid, solanesol, alkaloid and rutin in tobacco is achieved, the extraction rates are all 100%, the four components are effectively separated through simple steps, the utilization rate of the tobacco resource is improved, at the same time the operation is simple, the organic solvent is saved, and the energy consumption is reduced.

Description

technical field [0001] The invention relates to a comprehensive extraction and separation technology of tobacco functional components, in particular to a method for simultaneous extraction and separation of tobacco chlorogenic acid, solanesol, nicotine and rutin. Background technique [0002] The medicinal value of tobacco has been discussed in Chinese herbal medicine and traditional Chinese medicine literature. Modern analytical chemistry has proved that tobacco is rich in a variety of biologically active functional components. Tobacco is the most abundant plant solanesol (1-4%) resource; the content of chlorogenic acid (0.2-5.4%) in tobacco leaves is equivalent to that of honeysuckle; The functional components are comparable; nicotine and its related substances in tobacco are very promising as future drug candidates for the treatment of Alzheimer's disease. Nicotine pesticide is a traditional "green pesticide" with high efficiency and low toxicity. Tobacco also contains ...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07C33/02C07C29/76C07D401/04C07H17/07C07H1/08
CPCC07C29/76C07C67/48C07D401/04C07H1/08C07H17/07C07C69/732C07C33/02
Inventor 杜咏梅张怀宝李晓芹张忠锋侯小东付秋娟王爱华
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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