Method for fermentation of L-glutamic acid by cottonseed cake meal hydrolysate

A technology of cottonseed cake powder and hydrolyzate, which is applied in the direction of fermentation, etc., to reduce the cost of fermentation, promote the growth and fermentation of bacteria, and improve the conversion rate of sugar and acid

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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to reduce the cost of fermentation raw materials in the fermentation of glutamic acid, etc., to provide a new type of organic nitrogen source cottonseed cake powder hydrolyzate for L-glutamic acid fermentation, by adding different concentrations of cottonseed cake powder hydrolyzate Measures to make it suitable for fermentation of L-glutamic acid in temperature-sensitive fermentation method and biotin sub-appropriate fermentation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Select temperature-sensitive L-glutamic acid strains;

[0015] (2) Preparation of fermentation medium: molasses 20ml / L, corn steep liquor 20ml / L, glucose 30g / L, cottonseed cake powder hydrolyzate 20ml / L, MnSO 4 30mg / L, MgSO 4 2g / L, K 2 HPO 3 30mg / L, FeSO 4 20mg / L, VH0.3mg / L, VB0.3mg / L; Among them, the content of amino nitrogen in the hydrolyzate of cottonseed cake flour is 1.6%.

[0016] ⑶, preparation of seed medium: glucose 30g / L, corn steep liquor 40ml / L, KH 2 PO 3 1g / L, MgSO 4 0.5g / L, MnSO 4 2.5mg, FeSO 4 5mg / L, methionine 0.5g / L, VH0.3mg / L, VB10.3mg / L, urea 0.05g / L.

[0017] (4) Insert the bacterial strain into the above-mentioned seed culture medium, and the inoculum size is 10%; at 33°C, pH is 6.8-7.2 and dissolved oxygen is 20-30%, cultivate it in a 5L automatic control fermenter for 14-16h to the right Several periods.

[0018] ⑸. After cultivation, the strains are inserted into the above-mentioned fermentation medium, and the inoculation amount ...

Embodiment 2

[0020] (1) Select temperature-sensitive L-glutamic acid strains;

[0021] (2) Preparation of fermentation medium: molasses 20ml / L, corn steep liquor 20ml / L, glucose 30g / L, cottonseed cake powder hydrolyzate 10ml / L, MnSO 4 30mg / L, MgSO 4 2g / L, K 2 HPO 3 30mg / L, FeSO 4 20mg / L, VH0.3mg / L, VB0.3mg / L; Among them, the content of amino nitrogen in the hydrolyzate of cottonseed cake flour is 1.6%.

[0022] ⑶, prepare seed culture medium; Glucose 30g / L, corn steep liquor 40ml / L, KH 2 PO 3 1g / L, MgSO 4 0.5g / L, MnSO 4 2.5mg, FeSO 4 5mg / L, methionine 0.5g / L, VH0.3mg / L, VB10.3mg / L, urea 0.05g / L.

[0023] (4) Insert the bacterial strain into the above-mentioned seed culture medium, and the inoculum size is 10%; at 33°C, pH is 6.8-7.2 and dissolved oxygen is 20-30%, cultivate it in a 5L automatic control fermenter for 14-16h to the right Several periods.

[0024] ⑸. After cultivation, the strains are inserted into the above-mentioned fermentation medium, and the inoculation amount...

Embodiment 3

[0026] (1) Select temperature-sensitive L-glutamic acid strains;

[0027] (2) Preparation of fermentation medium; molasses 20ml / L, corn steep liquor 20ml / L, glucose 30g / L, cottonseed cake powder hydrolyzate 30ml / L, MnSO 4 30mg / L, MgSO 4 2g / L, K 2 HPO 3 30mg / L, FeSO 4 20mg / L, VH0.3mg / L, VB0.3mg / L; Among them, the content of amino nitrogen in the hydrolyzate of cottonseed cake flour is 1.6%.

[0028] ⑶, prepare seed culture medium; Glucose 30g / L, corn steep liquor 40ml / L, KH 2 PO 3 1g / L, MgSO 4 0.5g / L, MnSO 4 2.5mg, FeSO 4 5mg / L, methionine 0.5g / L, VH0.3mg / L, VB10.3mg / L, urea 0.05g / L.

[0029] (4) Insert the bacterial strain into the above-mentioned seed culture medium, and the inoculum size is 10%; at 33°C, pH is 6.8-7.2 and dissolved oxygen is 20-30%, cultivate it in a 5L automatic control fermenter for 14-16h to the right Several periods.

[0030] ⑸. After cultivation, the strains are inserted into the above-mentioned fermentation medium, and the inoculation amount...

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Abstract

The invention relates to a method for fermentation of L-glutamic acid by a cottonseed cake meal hydrolysate. The method is characterized in that: the cottonseed cake meal hydrolysate is added into an L-glutamic acid fermentation medium, amino nitrogen concentration of the cottonseed cake meal hydrolysate is 0.5-3.0%, the L-glutamic acid fermentation medium is a temperature sensitive L-glutamic acid fermentation medium, and the adding amount of the cottonseed cake meal hydrolysate is 15-25ml/l. By controlling the adding amount of the cottonseed cake meal hydrolysate, smooth L-glutamic acid fermentation can be effectively guaranteed. Utilization of the cottonseed cake meal hydrolysate not only broadens the organic nitrogen source resources, but also decreases the fermentation cost, so that the cottonseed cake meal hydrolysate has broad application prospects in the fermentation industry.

Description

technical field [0001] The invention relates to a method for fermenting L-glutamic acid with hydrolyzate of cottonseed cake powder, and belongs to the technical field of amino acid production by fermentation. Background technique [0002] In 2012, the growth rate of my country's bio-fermentation industry slowed down. The total product output reached 23 million tons, an increase of 6.0% year-on-year, and the total industrial output value reached more than 270 billion yuan, an increase of 8% year-on-year. Among them, the industries of amino acids, enzyme preparations, and functional fermentation products have maintained double-digit growth. At the same time, one of the main problems facing the industry is the decline in corporate profits due to rising costs and low prices. This requires continuous technological innovation to cope with costs. improvement and market expansion. In the fermentation production process, a large amount of organic nitrogen source is needed, which can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
Inventor 陈宁梁静波温冰徐庆阳谢希贤张成林刘淑云
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