A kind of strong emulsifying microbial anti-wax agent and its application
A technology of wax fungicide and emulsification, which is applied in the field of strong emulsifying microbial wax removal and anti-wax fungus agent, which can solve the problems of slow paraffin speed, short contact time, and limited wax-proof effect
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Embodiment 1
[0032] In the present embodiment, the strong emulsifying microbial anti-wax agent is composed of Pseudomonas aeruginosa N1 (CGMCC 1.2814), Pseudomonas aeruginosa N2 (CGMCC 1.10452) and surface bacillus (CGMCC 1.2673), 3 strains All were purchased from China General Microorganism Culture Collection Management Center (CGMCC). The bacterial agents are solid dry powders. The three bacteria are stored separately, and skim milk is used as the protective agent and carrier. The mass ratio of the three bacterial dry powders is 1:1:1. .
[0033] In the present embodiment, the application of the strong emulsifying microbial anti-wax agent in oil production comprises the following steps:
[0034] (1) Activate each strain separately until the cell concentration of each strain reaches 3.2~4.6×10 8 Each bacterial solution (2L) was mixed in equal volumes, and then the compound bacterial solution was added to the fermentation medium at a volume ratio of 1:10, fermented and cultivated at 40°C ...
Embodiment 2
[0040] In the present embodiment, the strong emulsifying microbial anti-wax agent is composed of Pseudomonas aeruginosa N1 (CGMCC 1.2814), Pseudomonas aeruginosa N2 (CGMCC 1.10452) and surface bacillus (CGMCC 1.2673), 3 strains All were purchased from China General Microorganism Culture Collection Management Center (CGMCC). The bacterial agents were in liquid state and stored separately. 8 individual / mL.
[0041] In the present embodiment, the application of the strong emulsifying microbial anti-wax agent in oil production comprises the following steps:
[0042] (1) Mix equal volumes of the bacteria liquids of various strains, transfer the compound bacterial liquids to the fermenter, add fermentation medium at a volume ratio of 1:10, ferment and cultivate at 35°C for 72 hours, and control the pH value at 6~ 8. There is no need to pass oxygen or air during the fermentation process. After the fermentation is completed, the next stage of fermentation is carried out. The volume r...
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