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Method of simultaneously extracting polyphenol, flavonoids and triterpenes from pomegranate flowers

A pomegranate flower and flavonoid technology, applied in the separation field, can solve the problems of waste and low utilization efficiency of pomegranate flower resources, and achieve the effect of high purity

Inactive Publication Date: 2014-11-05
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem solved by the present invention is that the resource utilization efficiency of pomegranate flower is low, only a certain component is extracted from pomegranate flower, while other components are discarded

Method used

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  • Method of simultaneously extracting polyphenol, flavonoids and triterpenes from pomegranate flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Take the dried pomegranate flower sample and use a stainless steel pulverizer to pulverize, the crushed particle size is 40 mesh, put the pulverized pomegranate flower sample powder into an extraction container that is resistant to acid corrosion, and then add an acidic methanol solution that is 10 times the mass of the sample (The mass ratio of concentrated hydrochloric acid is 2%), and extract by heating and refluxing in a water bath at 65°C for 2 hours. The extract was filtered under reduced pressure, the filtrate was concentrated under reduced pressure at 60°C, and methanol was recovered to obtain reddish-brown dilute extract. (2) Add 3 times the volume of water to the reddish-brown dilute extract, stir well to dissolve, and let stand at 4°C for 10 hours. (3) Separate the supernatant and the precipitate, add an equal amount of n-hexane to the supernatant for extraction, shake well and mix several times, and use a liquid separator to separate the aqueous phase an...

Embodiment 2

[0044] (1) Take the dried pomegranate flower sample and use a stainless steel pulverizer to pulverize it with a particle size of 60 mesh. Put the pulverized pomegranate flower sample powder into an extraction container that is resistant to acid corrosion, and then add an acidic methanol solution that is 12 times the mass of the sample. (The mass ratio of concentrated hydrochloric acid is 2.5%), and the temperature is 61 °C under the condition of ultrasonic assisted reflux extraction for 1 hour, and the ultrasonic power is 100W. The extract was filtered under reduced pressure, the filtrate was concentrated under reduced pressure at a temperature of 50°C, and methanol was recovered to obtain reddish-brown dilute extract. (2) Add 5 times the volume of water to the reddish-brown dilute extract, stir well to dissolve, and let stand at 5°C for 12 hours. (3) Separate the supernatant and the precipitate, add an equal amount of ethyl acetate to the supernatant for extraction, shake wel...

Embodiment 3

[0046] (1) Take the dried pomegranate flower sample and use a stainless steel pulverizer to pulverize, the crushed particle size is 50 mesh, put the pulverized pomegranate flower sample powder into an extraction container that is resistant to acid corrosion, and then add an acidic methanol solution that is 11 times the mass of the sample (The mass ratio of concentrated hydrochloric acid is 3%), reflux extraction for 1.5 hours under ultrasonic-assisted conditions at a temperature of 62°C, and the ultrasonic power is 100W. The extract was filtered under reduced pressure, the filtrate was concentrated under reduced pressure at a temperature of 55°C, and methanol was recovered to obtain reddish-brown dilute extract. (2) Add 4 times the volume of water to the reddish-brown dilute extract, stir well to dissolve, and let stand at 4.5°C for 11 hours. (3) Separate the supernatant and the precipitate, add an equal amount of ethyl acetate to the supernatant for extraction, shake well and...

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Abstract

The invention relates to a method of simultaneously extracting polyphenol, flavonoids and triterpenes from pomegranate flowers. The method comprises the steps: with the pomegranate flowers as raw materials, extracting the pomegranate flowers by virtue of a methanol solution containing 2-3 percent concentrated hydrochloric acid, concentrating an extract, dissolving by water, enabling the polyphenol and the flavonoids to enter an aqueous solution and the triterpenes to be left in a precipitate, carrying out extraction on the aqueous solution by an organic solvent, concentrating and drying a water phase after extraction to obtain a polyphenol extract, treating an organic phase by a recovery solvent, drying to obtain a flavonoids extract, dissolving the precipitate part by alkaline ethanol with a pH value of 8-9, filtering, regulating a pH value of a filtrate to 5-6, recovering ethanol, and drying the prepared precipitate part to obtain a triterpenes extract. With the adoption of the method, the pomegranate flower resource can be more effectively utilized, and the polyphenol, the flavonoids and the triterpenes are simultaneously extracted; the purpose of extracting the three active components from a batch of pomegranate flowers is reached; and the yield and the purity of the prepared products are both higher, the traditional commercial needs can be satisfied, and the pomegranate flower resource is fully and reasonably utilized.

Description

technical field [0001] The invention belongs to the field of separation methods, in particular to a method for simultaneously extracting polyphenols, flavonoids and triterpenoids from pomegranate flowers. Background technique [0002] Pomegranate ( Punica granatum L.) is a plant of the genus Pomegranate of the family Pomegranate. The place of origin is Central Asia, and there are introduced species in some parts of China. All parts of pomegranate have medicinal value. The peel of pomegranate has astringent intestinal diarrhea, hemostasis, and insect repellent effects; Water extract and pomegranate juice have strong anti-oxidation and lipid-regulating effects. Pomegranate flower can be used to treat otitis media, and is used to treat diabetes and its complications in India and other countries. [0003] Studies in recent years have found that pomegranate flowers are rich in active ingredients, including polyphenols, flavonoids, triterpenoids and polysaccharides, among whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K133/00
Inventor 张立华孙蕾赵登超
Owner SHANDONG FOREST SCI RES INST
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