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Application of Fe5C2 nano-particle to prepare bioimaging probe

A nanoparticle, biological imaging technology, applied in MRI/MRI contrast agents, preparations for in vivo experiments, wave energy or particle radiation treatment materials, etc. complex issues

Active Publication Date: 2014-11-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, although Tian Q. et al. passed the magnetic Fe 3 o 4 The method of growing strong light-absorbing copper sulfide on the surface of nanoparticles enables all functions to be realized in one nanoparticle, but this method still requires multi-step preparation, and the process is very complicated; and Fe 3 o 4 Easily oxidized in aqueous solution, thus changing its magnetic properties

Method used

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  • Application of Fe5C2 nano-particle to prepare bioimaging probe
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  • Application of Fe5C2 nano-particle to prepare bioimaging probe

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Embodiment 1

[0063] Embodiment 1 Bioimaging probe provided by the present invention

[0064] 1. The preparation process of bioimaging probe:

[0065] 1) Fe 5 C 2 Synthesis of nanoparticles: a mixture of octadecylamine and cetyltrimethylammonium bromide was prepared in N 2 Under protection, heat up to 120°C for dehydration, inject 0.5mL containing 3.6mmol Fe(CO) 5 . Raise the temperature to 180°C at a speed of 10°C / min, and after holding for 10 minutes, raise the temperature to 350°C at a speed of 10°C / min, continue to keep warm for 10 minutes, and then cool down to room temperature. After the product was collected by centrifugation at 10000rpm, the solution was washed 3 times with a mixed solution of ethanol and n-hexane, and the product Fe 5 C 2 The nanoparticles were dispersed in n-hexane to obtain Fe 5 C 2 Nanoparticles in n-hexane solution. Figure 17 shows that the Fe 5 C 2 TEM image of the nanoparticles, showing a core-shell structure. Figure 18 is Fe 5 C 2 High-resolu...

Embodiment 2

[0082] Example 2 The photoacoustic imaging-guided photothermotherapy probe provided by the present invention

[0083] 1. Probe preparation process:

[0084] Photoacoustic imaging-guided photothermotherapy probes (i.e., Fe 5 C 2 -PEG-Z HER2:342 ) preparation: 100μL 1mM Z HER2:342 The solution was dissolved in 10 mL of phosphate buffer (PBS, pH=7.4), and 30 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC HCl) and 35 mg of N-hydroxy Sulfosuccinimide (NHS-sulfo), stirred and activated for 1 hour, then added 20 mg modified DSPE-PEG-NH 2 Obtained Fe 5 C 2 - PEG nanoparticles, continue to stir for 4h. Centrifuge at 20,000 rpm for 15 minutes to collect the product, and dialyze for 24 hours to remove unreacted Z HER2:342 and EDC HCl, NHS-sulfo, the photoacoustic imaging-guided photothermotherapy probe Fe can be obtained 5 C 2 -PEG-Z HER2:342 , the biological imaging is magnetic resonance imaging and / or photoacoustic imaging.

[0085] 2. Fe 5 C 2 -PE...

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Abstract

The invention provides application of Fe5C2 nano-particle to prepare a bioimaging probe. Fe5C2 nano-particle usually used as a Fischer-Tropsch synthesis catalyst is employed as a raw material, and a simple method is employed for obtaiing the bioimaging probe with stable performance. The bioimaging probe is capable of providing imaging data of tumor positions for various tumor diagnosis and treatment means, and further assisting and controlling the diagnosis and treatment process of tumor. The invention also provides application of Fe5C2 nano-particle to prepare a bioimaging-introduced photothermal therapy probe. By coupling a tumor-cell-specific affinity ligand to Fe5C2 nano-particle, the probe is capable of specifically targeting a specific tumor cell and realizing selective killing on the specific tumor cell, and also the treatment effect on tumor can be known and controlled through bioimaging.

Description

technical field [0001] The present invention relates to a kind of Fe 5 C 2 The application of nanoparticles, especially related to a kind of Fe 5 C 2 Application of nanoparticles in the preparation of bioimaging probes. Background technique [0002] In recent years, cancer has become an important cause of death worldwide. Photothermal therapy is attracting more and more attention as a minimally invasive local tumor treatment method. Agents are usually nano-reagents, such as gold nanorods, nanostars, nanocages, graphene, carbon nanotubes, etc. [0003] Although the above-mentioned nano-reagents play an important role in photothermal therapy, it is more important to understand their metabolic pathways and the way they function, and it is also the key to determine the tumor treatment plan and understand and control the tumor treatment effect. This problem can be better solved by means of biological imaging. Among the many imaging methods, photoacoustic imaging is often u...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/06A61K49/12A61K41/00
Inventor 侯仰龙余靓杨策
Owner PEKING UNIV
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