Preparation method of racemic theanine

A theanine and racemate technology, which is applied in the field of chemical method racemization of theanine, can solve the problems of difficulty in obtaining D-theanine standard samples, hindering the research work of theanine, and failing to prepare pure D-type products, etc. To achieve the effect of simple operation, elimination or reduction of D-type content, and reduction of toxicity

Inactive Publication Date: 2014-11-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, D-theanine standard samples are difficult to obtain
Moreover, there is currently no literature related to the above-mentioned method for determining the distribution of DL, and there is no report on the preparation of pure D-type products, which has greatly hindered the research on theanine.

Method used

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  • Preparation method of racemic theanine
  • Preparation method of racemic theanine
  • Preparation method of racemic theanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: 10g theanine is dissolved in 200ml acetic acid, add 0.2ml salicylaldehyde. Heat in a water bath at 80°C, stir, take 5ml of the reaction solution every 10min and dilute 25ml to measure the optical rotation. After 70min, the measured optical rotation is 0. After drying by rotary evaporation, wash with ethanol and suction filter to generate racemic theanine.

Embodiment 2

[0048] Example 2: 10 g of theanine was dissolved in 200 ml of acetic acid, and 0.2 ml of salicylaldehyde was added. Heat and stir in a water bath at 85°C, take 5ml of the reaction solution every 10min and dilute 25ml to check the optical rotation. After 40 minutes, 5ml was diluted to 25ml and the optical rotation was measured as 0. After drying by rotary evaporation, it was washed with ethanol and suction filtered to generate racemic theanine.

Embodiment 3

[0049] Example 3: 10 g of theanine was dissolved in 200 ml of acetic acid, and 0.2 ml of salicylaldehyde was added. Heat and stir in a water bath at 90°C, take 5ml of the reaction solution every 10min and dilute 25ml to check the optical rotation. After 40 minutes, 5ml was diluted to 25ml and the optical rotation was measured as 0. After drying by rotary evaporation, it was washed with ethanol and suction filtered to generate racemic theanine.

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Abstract

The invention discloses a preparation method of racemic theanine. The preparation method comprises the following steps: dissolving L-theanine into organic acid, adding aldehyde, heating in a water bath, completing reaction, and performing after-treatment to obtain racemic theanine. Compared with the prior art, the preparation method disclosed by the invention has the beneficial effects that a racemic theanine standard product can be obtained by adopting the method; the standard product can be conveniently used for analyzing the distribution conditions of a to-be-tested sample D and L-theanine so as to conveniently remove or reduce the D-type content, improve the product quality and biological function, and reduce the toxicity; moreover, the preparation method is simple in operation and mild in reaction condition, adopts a non-toxic and non-corrosive reagent, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of amino acid racemization, and in particular relates to a chemical method for racemization of theanine, and the HPLC method is used to detect the purity and chirality of theanine racemite. Background technique [0002] Theanine (L-theanine) is a characteristic non-protein amino acid of tea. It has physiological effects such as weight loss and blood pressure reduction, anti-tumor, nerve protection, fatigue relief, sleep improvement, and immunity enhancement. It is recognized as a safe substance and a natural medicine , natural health care products, functional beverages and other fields with the most development potential substances. Its structural formula is: [0003] [0004] Chiral molecules interact with biological macromolecules such as enzymes, receptors, and ion channels in the human body, showing intricate enantioselectivity. Therefore, the pharmacological effects, metabolic processes, and toxicit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/24
Inventor 王友明李奎龚杜明王建凤邵强王迎松汪以真刘波静王霖
Owner ZHEJIANG UNIV
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