Method for producing iturin through liquid state fermentation with rapeseed meal as raw material

An iturin and liquid state fermentation technology, applied in the field of iturin production by liquid state fermentation, achieves the effects of reduced sinapine content, easy industrial scale-up, and simple operation

Inactive Publication Date: 2014-11-12
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of untreated cheap rapeseed meal as a nitrogen source for liquid fermentation to produce iturin has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for producing iturin by liquid fermentation of rapeseed meal as raw material, inserting bacillus producing iturin into a medium with rapeseed meal as a nitrogen source, using 4mol / L sodium hydroxide solution, 4mol / L dilute sulfuric acid solution to adjust the pH of the fermentation medium, liquid fermentation culture, respectively collect fermentation broth and rapeseed residue, including the following steps:

[0019] 1) Strain activation: Bacillus subtilis3-10 (GeneBank accession number: JF460845, gifted by Professor Chen Shouwen of Huazhong Agricultural University) was first cultured in an incubator at 28°C for 24 hours, and the medium was LB agar medium , to obtain activated Bacillus subtilis;

[0020] 2) Shake flask culture: Pick a single colony of activated Bacillus subtilis in LB liquid medium for shake flask culture for 14 hours at a temperature of 28°C and a rotation speed of 220rpm;

[0021] 3) fermentor cultivation: the Bacillus subtilis cultivated ...

Embodiment 2

[0026] A method for producing iturin by liquid fermentation of rapeseed meal as raw material, inserting bacillus producing iturin into a medium with rapeseed meal as a nitrogen source, using 4mol / L sodium hydroxide solution, 4mol / L dilute sulfuric acid solution to adjust the pH of the fermentation medium, liquid fermentation culture, respectively collect fermentation broth and rapeseed residue, including the following steps:

[0027] 1) Strain activation: Bacillus subtilis3-10 (GeneBank accession number: JF460845, gifted by Professor Chen Shouwen of Huazhong Agricultural University) was first cultured in an incubator at 28°C for 24 hours, and the medium was LB agar medium , to obtain activated Bacillus subtilis;

[0028] 2) Shake flask culture: Pick a single colony of activated Bacillus subtilis in LB liquid medium for shake flask culture for 14 hours at a temperature of 28°C and a rotation speed of 220rpm;

[0029] 3) fermentor cultivation: the Bacillus subtilis cultivated ...

Embodiment 3

[0034] A method for producing iturin by liquid state fermentation of rapeseed meal as raw material, comprising the following steps:

[0035] 1) Strain activation: Bacillus subtilis3-10 (GeneBank accession number: JF460845, gifted by Professor Chen Shouwen of Huazhong Agricultural University) was first cultured in an incubator at 28°C for 24 hours, and the medium was LB agar medium , to obtain activated Bacillus subtilis;

[0036] 2) Shake flask culture: Pick a single colony of activated Bacillus subtilis in LB liquid medium for shake flask culture for 14 hours at a temperature of 28°C and a rotation speed of 220rpm;

[0037] 3) fermentor cultivation: the Bacillus subtilis cultivated by the shake flask is fermented in the fermentation medium (in the 7L fermentor), and the fermentation condition is: inoculum size 2% (v / v) (the Bacillus subtilis cultivated by the shake flask The volume is 2% of the initial fermentation medium volume of the fermenter), pH7.0, the fermentation ven...

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Abstract

The invention belongs to the field of biotechnology. A method for producing iturin through liquid state fermentation with rapeseed meal as a raw material is characterized by including the following steps of firstly, activating strains to obtain activated bacillus subtilis; secondly, conducting shake-flask culture; thirdly, conducting culture in a fermentation cylinder, wherein the bacillus subtilis where shake-flask culture is conducted is fermented in a fermentation medium, and the fermentation conditions include that the inoculum size ranges from 2%(v/v) to 10%(v/v), the pH is 7.0, the fermentation to ventilation ratio is 1-2:1 (vvm), the stirring rotation speed ranges from 300 rpm to 800 rpm, and the fermentation cycle is 72 hours; fourthly, collecting fermentation supernatant and rapeseed meal residues. While high-concentration iturin is obtained through liquid state fermentation with the rapeseed meal as a nitrogen source, the rapeseed meal residues which are appropriately dried can be used as forage protein rich in probiotics and can also be used as bio-organic fertilizers with the bacteriostasis function, and therefore high-valued full utilization of rapeseed meal resources is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing iturin by liquid-state fermentation of rapeseed meal as a raw material. Background technique [0002] Organic nitrogen sources such as by-products of agricultural product processing such as soybean meal, peanut meal, and corn gluten meal have been widely used in the fermentation process of various biological products. Among them, soybean meal and its derivatives are the best nitrogen sources for industrial fermentation of many microbial secondary metabolites (such as antibiotics). Compared with soybean meal, rapeseed meal is a cheaper protein resource, but there are relatively few reports on its direct bioutilization as a fermentation raw material. Early research mainly focused on the reports on the production of various enzyme preparations by solid-state fermentation of rapeseed meal as a substrate. Rapeseed meal is a non-soluble slow-release nitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/125
Inventor 黄凤洪金虎钮琰星李坤朋张欣然郭勉万楚筠李文林胡传炯
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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