Preservation method of gerbera germplasm resources

Abstract

The invention discloses a preservation method of gerbera germplasm resources. The method comprises the following steps: selecting gerbera flowers during the period from blooming of ray florets to pollination of tubiform florets; keeping flower stalks in the length of 5-6 cm; carrying out induction culture on treated explants; continuously sub-culturing through two subculture mediums with different prescriptions; carrying out rooting culture in a greenhouse; and culturing in a seedling-growing bag and growing for one year in the greenhouse. The gerbera germplasm resources can be preserved for 3-4 years in one preservation cycle and the field survival rate of germplasm resource materials reaches more than 90%. According to the preservation method, the gerbera germplasm resources are preserved through successive subculture of the two different subculture mediums and renovation in the greenhouse, the problems of germchit variation and quality degradation caused by hormone factors, culture environments and the like are reduced, the excellent traits of the gerbera germplasm resources are stably preserved and a new and effective preservation method of the gerbera germplasm resources can be provided.

Description

technical field [0001] The invention relates to a method for preserving gerbera germplasm resources, and belongs to the field of biotechnology. Background technique [0002] Gerbera (Gerbera jamesonil Bolus), also known as Gerbera, is a perennial evergreen herbaceous flower of the genus Gerbera in the family Asteraceae. Because of its huge flowers, rich colors, different postures, strong decoration, and long-distance transportation resistance, it is favored by consumers. Under suitable conditions, it can produce and bloom every year, and can supply fresh cut flowers to the market throughout the year. It is one of the top five cut flowers in the world. my country has introduced and cultivated a large number of varieties from the Netherlands since the 1990s. After years of experiments, due to the limitations of climatic conditions and cultivation techniques, quality degradation has generally occurred, and excellent varieties have been seriously degraded or even lost, and have...

AI Technical Summary

Problems solved by technology

[0004] At present, most germplasm resources of gerbera are preserved by indoor tissue culture and field cultivation. The tissue culture method mainly uses young bud receptacles as explants for tissue culture of gerbera, but it is difficult to disinfect the surface of young bud receptacles as explants. , the consumption of test materials is large, and the size of flower buds is strictly required. If it is too small, it is easy to die, and if it is too large, the rate of dyeing is too large. The lack of cultivation and preservation is manifested in the short subculture period and relatively high cost of species preservation; the deficiency of cultivation and preservation in sheds is manifested in the large area occupied, the difficulty in controlling light, temperature, water and pests and diseases, and the formation of soil compaction, Salinization, accumulation of pathogenic bacteria, and continuous cropping obstacles lead to the loss of original typicality of varieties

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