Preservation method of gerbera germplasm resources

A preservation method and gerbera technology, applied in the field of gerbera germplasm resources preservation, can solve the problems of strict flower bud size requirements, loss of typical varieties, short subculture period, etc., to reduce the probability of variation and browning rate and mortality, the effect of long flowering

Active Publication Date: 2014-11-19
玉溪云星生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most germplasm resources of gerbera are preserved by indoor tissue culture and field cultivation. The tissue culture method mainly uses young bud receptacles as explants for tissue culture of gerbera, but it is difficult to disinfect the surface of young bud receptacles as explants. , the consumption of test materials is large, and the size of flower buds is strictly required. If it is too small, it is easy to die, and if it is too large, the rate of dyeing is too larg

Method used

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  • Preservation method of gerbera germplasm resources
  • Preservation method of gerbera germplasm resources
  • Preservation method of gerbera germplasm resources

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1

[0046] (1) Selection and pretreatment of explant materials

[0047] ①Choose a robust gerbera plant with good plant type, no pest damage, and germplasm consistent with the parent, pick the gerbera flowers that have just opened ligulate flowers, leave the pedicels 5cm-6cm long, and insert a 5mg / L 6 - BA aqueous solution (that is, 6-BA is prepared with tap water into an aqueous solution with a concentration of 5 mg / L), placed in a refrigerator at 4°C for low-temperature treatment for 7 days;

[0048]② Wash the material treated in step (1)① with washing powder and water, and then rinse it with clean water. In an ultra-clean environment, use a blade to cut off the pedicel and the edge of the lignus (the size of the material is suitable for disinfection in a container). ), put into the mercuric chloride solution that mass percentage concentration is 0.1% for sterilization 15min, then put into the mixed solution that adds 2 drops of Tween 20 in the sodium hypochlo...

Example Embodiment

[0075] Example 2

[0076] Except that the following operations are different in Embodiment 2, other operations are the same as those in Embodiment 1, and will not be repeated here.

[0077] (1) Selection and pretreatment of explant materials

[0078] ①Choose a robust gerbera plant with good plant type, no pest damage, germplasm consistent with the parent, pick the gerbera flowers whose ligular flowers have fully opened, but the tube-shaped flowers have not yet spit out powder, and the pedicel length is 5cm ~6cm, inserted into the aqueous solution containing 10mg / L 6-BA, and placed in a refrigerator at 4°C for low-temperature treatment for 5 days;

[0079] (2) Induction culture

[0080] Induction medium:

[0081] MS basic medium

[0082]

[0083] (3) The first subculture

[0084] Subculture medium I:

[0085] MS modified medium

[0086]

[0087]

[0088] (4) Second subculture

[0089] Subculture medium II:

[0090] MS modified medium

[0091]

Example Embodiment

[0092] Example 3

[0093] Except that the following operations are different in Embodiment 3, other operations are the same as those in Embodiment 1, and will not be repeated.

[0094] (1) Selection and pretreatment of explant materials

[0095] ①Choose robust gerbera plants with good plant type, no pest damage, germplasm consistent with the parent, pick the gerbera flowers whose tubular flowers have spit powder, leave the pedicels to be 5cm-6cm long, and insert a 15mg / L6 - BA aqueous solution, placed in a refrigerator at 4°C for low temperature treatment for 3 days;

[0096] (2) Induction culture

[0097] Induction medium:

[0098] MS basic medium

[0099]

[0100] (3) The first subculture

[0101] Subculture medium I:

[0102] MS modified medium

[0103]

[0104] (4) Second subculture

[0105] Subculture medium II:

[0106] MS modified medium

[0107]

[0108] The above embodiments can preserve Gerbera germplasm resources for more than 3 to 4 years, and th...

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Abstract

The invention discloses a preservation method of gerbera germplasm resources. The method comprises the following steps: selecting gerbera flowers during the period from blooming of ray florets to pollination of tubiform florets; keeping flower stalks in the length of 5-6 cm; carrying out induction culture on treated explants; continuously sub-culturing through two subculture mediums with different prescriptions; carrying out rooting culture in a greenhouse; and culturing in a seedling-growing bag and growing for one year in the greenhouse. The gerbera germplasm resources can be preserved for 3-4 years in one preservation cycle and the field survival rate of germplasm resource materials reaches more than 90%. According to the preservation method, the gerbera germplasm resources are preserved through successive subculture of the two different subculture mediums and renovation in the greenhouse, the problems of germchit variation and quality degradation caused by hormone factors, culture environments and the like are reduced, the excellent traits of the gerbera germplasm resources are stably preserved and a new and effective preservation method of the gerbera germplasm resources can be provided.

Description

technical field [0001] The invention relates to a method for preserving gerbera germplasm resources, and belongs to the field of biotechnology. Background technique [0002] Gerbera (Gerbera jamesonil Bolus), also known as Gerbera, is a perennial evergreen herbaceous flower of the genus Gerbera in the family Asteraceae. Because of its huge flowers, rich colors, different postures, strong decoration, and long-distance transportation resistance, it is favored by consumers. Under suitable conditions, it can produce and bloom every year, and can supply fresh cut flowers to the market throughout the year. It is one of the top five cut flowers in the world. my country has introduced and cultivated a large number of varieties from the Netherlands since the 1990s. After years of experiments, due to the limitations of climatic conditions and cultivation techniques, quality degradation has generally occurred, and excellent varieties have been seriously degraded or even lost, and have...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 单芹丽杨春梅李绅崇王继华吴丽芳汪国鲜黎霞赵培飞吴旻
Owner 玉溪云星生物科技有限公司
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