The method of rapid propagation of seedlings of artichoke test-tube seedlings

Abstract

The invention discloses a stem tip rapid propagation seedling test-tube culturing method for cynara scolymus L. According to the invention, seeds are sown in a test tube after being sterilized so as to obtain a great quantity of aseptic seedlings; stem tips of the test tube seedlings, which have suitable sizes, are adopted to carry out tufted-bud induction so as to obtain successive rootless seedlings; the rootless seedlings are subjected to rooting cultivation to obtain artichoke high-quality seedlings. Seed coats of cynara scolymus L. are hard and cynara scolymus L. has strong tolerance to a disinfectant, and thus, after the disinfectant adopted by the stem tip rapid propagation seedling culturing method is used for disinfecting, the non-pollution rate can reach over 80 percent; the seeds subjected to proper physical processing have a germination rate of over 85 percent after being disinfected. The obtained aseptic seedlings are subjected to stem tip induction, have a survival rate of 90 percent and have a tufted-bud induction of 85 percent; after the seeds are subjected to test tube sowing to form the seedlings, the seedlings do not need to be disinfected and the success rate can reach over 90 percent, and thus, undoubtedly, the aims of rapidly propagating the seedlings and reducing cost are fulfilled. The method disclosed by the invention has the advantages of high seedling rapid propagation efficiency, low cost, wide prospect and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for rapidly propagating seedlings of artichoke test-tube seedlings. Background technique [0002] Artichoke, also known as chrysanthemum thistle and artichoke, scientific name Cynarascolymus L., is a perennial herb of the genus Artichoke in the family Comopsite. And it is a kind of medicinal and edible plant, which is not only a good dish, but also has good medicinal value. Its buds or leaves contain styrenin, styrenin, bigmachinin, luteolin, etc. Ingredients, in European countries such as Germany, France, Britain, Italy, artichoke is used to prevent and treat indigestion, constipation, diarrhea, liver and gallbladder diseases, hyperlipidemia, arteriosclerosis, hyperglycemia, jaundice, etc.; in Brazil, artichoke For the prevention of diabetes and high blood pressure. At the same time, the most prominent feature of artichoke is that it has the function of protec...

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Problems solved by technology

[0003] At present, the planting methods of artichoke mostly adopt seed sowing and open-air planting, and artichoke is an alienated pollinated plant, which only blooms but does not bear fruit in most areas of my country, and the seed breeding rate is low. Although the seeds are expensive, several major planting areas of my country (such as Hunan) Changde) needs to import a large number of seeds every year, which is not only costly, but also often delays the farming time because the stubble cannot be connected; the other is through tissue culture and rapid propagation technology, but the explants are the tiller shoot tips of strong plants in the open field After disinfection, the artichoke seedlings are induced and cultivated. The disadvantages and shortcomings are that the surface of the artichoke is densely covered with fluff, and the disinfection effect is not ideal, and the young stem tips are often browned or dead due to the side effects of disinfectants, which affects the seedling rate (Zhang Pingxi , Tissue Culture and Rapid Propagation Technology of Artichoke [J]. Chinese Horticultural Digest, 2011.6)

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